TY - JOUR
T1 - Dehydrothermally Cross-Linked Collagen Membrane with a Bone Graft Improves Bone Regeneration in a Rat Calvarial Defect Model
AU - An, Yin Zhe
AU - Heo, Young Ku
AU - Lee, Jung Seok
AU - Jung, Ui Won
AU - Choi, Seong Ho
N1 - Publisher Copyright:
© 2017 by the authors.
PY - 2017/8/10
Y1 - 2017/8/10
N2 - In this study, the bone regeneration efficacy of dehydrothermally (DHT) cross-linkedcollagen membrane with or without a bone graft (BG) material was evaluated in a critical-sized ratmodel. An 8-mm-diameter defect was created in the calvaria of 40 rats, which were randomized intofour groups: (1) control; (2) DHT; (3) BG; and, (4) DHT + BG. Evaluations were made at 2 and 8 weeksafter surgery using micro-computed tomographic (micro-CT), histological, and histomorphometricanalyses. Micro-CT analysis showed an increase in the new bone volume (NBV) of the BG and DHT+ BG groups at 2 weeks after surgery, representing a significant difference (p < 0.05). At 8 weeksafter surgery, the NBV increased in all four groups. However, larger NBVs were observed in theBG and DHT + BG groups, and a significant difference was no longer observed between the twogroups. Histologic analysis demonstrated that the graft materials sustained the center of the defectin the BG and DHT + BG groups, which was shown in histomorphometric analysis as well. Theseresults suggest that DHT membrane is a safe biomaterial with adequate tissue integration, and hasa positive effect on new bone formation. Moreover, the best effects were achieved when DHT wasused in conjunction with BG materials.
AB - In this study, the bone regeneration efficacy of dehydrothermally (DHT) cross-linkedcollagen membrane with or without a bone graft (BG) material was evaluated in a critical-sized ratmodel. An 8-mm-diameter defect was created in the calvaria of 40 rats, which were randomized intofour groups: (1) control; (2) DHT; (3) BG; and, (4) DHT + BG. Evaluations were made at 2 and 8 weeksafter surgery using micro-computed tomographic (micro-CT), histological, and histomorphometricanalyses. Micro-CT analysis showed an increase in the new bone volume (NBV) of the BG and DHT+ BG groups at 2 weeks after surgery, representing a significant difference (p < 0.05). At 8 weeksafter surgery, the NBV increased in all four groups. However, larger NBVs were observed in theBG and DHT + BG groups, and a significant difference was no longer observed between the twogroups. Histologic analysis demonstrated that the graft materials sustained the center of the defectin the BG and DHT + BG groups, which was shown in histomorphometric analysis as well. Theseresults suggest that DHT membrane is a safe biomaterial with adequate tissue integration, and hasa positive effect on new bone formation. Moreover, the best effects were achieved when DHT wasused in conjunction with BG materials.
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U2 - 10.3390/ma10080927
DO - 10.3390/ma10080927
M3 - Article
AN - SCOPUS:85027435105
VL - 10
JO - Materials
JF - Materials
SN - 1996-1944
IS - 8
M1 - 927
ER -