Abstract
DICE1 (deleted in cancer 1), first identified in human lung carcinoma cell lines, is a candidate tumor suppressor, but the details of its activity remain largely unknown. We have found that RNA interference of its C. elegans homolog (DIC-1) produced inviable embryos with increased apoptosis, cavities in cells and abnormal morphogenesis. In the dic-1(RNAi) germ line, ced-3-dependent apoptosis increased, and cell cavities appeared at the late-pachytene/oogenic stage, leading to defective oogenesis. Immunofluorescence microscopy of DIC-1 revealed its ubiquitous expression in the form of cytoplasmic foci, and cryoelectron microscopy narrowed down the location of the foci to the inner membrane of mitochondria. After dic-1 RNAi, mitochondria had an irregular morphology and contained numerous internal vesicles. Homozygous embryos from a heterozygous dic-1 mother arrested at the L3 larval stage, in agreement with the essential role of DIC-1 in mitochondria. In summary, C. elegans DIC-1 plays a crucial role in the formation of normal morphology of the mitochondrial cristae/inner membrane. Our results suggest that human DICE1 may have several functions in multiple intracellular locations.
| Original language | English |
|---|---|
| Pages (from-to) | 3597-3606 |
| Number of pages | 10 |
| Journal | Development |
| Volume | 133 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - 2006 Sep 1 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Developmental Biology
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Deleted in cancer 1 (DICE1) is an essential protein controlling the topology of the inner mitochondrial membrane in C. elegans. / Han, Sung Min; Lee, Tae Hoon; Mun, Ji Young; Kim, Moon Jeong; Kritikou, Ekaterini A.; Lee, Se Jin; Han, Sung Sik; Hengartner, Michael O.; Koo, Hyeon Sook.
In: Development, Vol. 133, No. 18, 01.09.2006, p. 3597-3606.Research output: Contribution to journal › Article
TY - JOUR
T1 - Deleted in cancer 1 (DICE1) is an essential protein controlling the topology of the inner mitochondrial membrane in C. elegans
AU - Han, Sung Min
AU - Lee, Tae Hoon
AU - Mun, Ji Young
AU - Kim, Moon Jeong
AU - Kritikou, Ekaterini A.
AU - Lee, Se Jin
AU - Han, Sung Sik
AU - Hengartner, Michael O.
AU - Koo, Hyeon Sook
PY - 2006/9/1
Y1 - 2006/9/1
N2 - DICE1 (deleted in cancer 1), first identified in human lung carcinoma cell lines, is a candidate tumor suppressor, but the details of its activity remain largely unknown. We have found that RNA interference of its C. elegans homolog (DIC-1) produced inviable embryos with increased apoptosis, cavities in cells and abnormal morphogenesis. In the dic-1(RNAi) germ line, ced-3-dependent apoptosis increased, and cell cavities appeared at the late-pachytene/oogenic stage, leading to defective oogenesis. Immunofluorescence microscopy of DIC-1 revealed its ubiquitous expression in the form of cytoplasmic foci, and cryoelectron microscopy narrowed down the location of the foci to the inner membrane of mitochondria. After dic-1 RNAi, mitochondria had an irregular morphology and contained numerous internal vesicles. Homozygous embryos from a heterozygous dic-1 mother arrested at the L3 larval stage, in agreement with the essential role of DIC-1 in mitochondria. In summary, C. elegans DIC-1 plays a crucial role in the formation of normal morphology of the mitochondrial cristae/inner membrane. Our results suggest that human DICE1 may have several functions in multiple intracellular locations.
AB - DICE1 (deleted in cancer 1), first identified in human lung carcinoma cell lines, is a candidate tumor suppressor, but the details of its activity remain largely unknown. We have found that RNA interference of its C. elegans homolog (DIC-1) produced inviable embryos with increased apoptosis, cavities in cells and abnormal morphogenesis. In the dic-1(RNAi) germ line, ced-3-dependent apoptosis increased, and cell cavities appeared at the late-pachytene/oogenic stage, leading to defective oogenesis. Immunofluorescence microscopy of DIC-1 revealed its ubiquitous expression in the form of cytoplasmic foci, and cryoelectron microscopy narrowed down the location of the foci to the inner membrane of mitochondria. After dic-1 RNAi, mitochondria had an irregular morphology and contained numerous internal vesicles. Homozygous embryos from a heterozygous dic-1 mother arrested at the L3 larval stage, in agreement with the essential role of DIC-1 in mitochondria. In summary, C. elegans DIC-1 plays a crucial role in the formation of normal morphology of the mitochondrial cristae/inner membrane. Our results suggest that human DICE1 may have several functions in multiple intracellular locations.
UR - http://www.scopus.com/inward/record.url?scp=33750367966&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750367966&partnerID=8YFLogxK
U2 - 10.1242/dev.02534
DO - 10.1242/dev.02534
M3 - Article
C2 - 16914495
AN - SCOPUS:33750367966
VL - 133
SP - 3597
EP - 3606
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 18
ER -