Delivery of interleukin-18 gene to lung cancer cells using cationic emulsion.

Hyun Suk Kang, Shun Ji Jin, Chang Seon Myung, Sung Joo Hwang, Jeong Sook Park

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Interleukin-18 (IL-18) is known to reduce melanoma lung metastases through various mechanisms. For the delivery of IL-18 gene into the lung, three different cationic emulsions as non-viral vectors were formulated using the same components of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-trimethylammonium propane (DOTAP), and Tween 80 with distinct oils. By using the small particle size of physicochemically stable E3, the complex of E3/plasmid DNA encoding IL-18 (16:2.5, w/w) was transfected into lung cancer cells, and the amount of plasmid DNA transferred and the expression of both mRNA and protein for IL-18 were measured. When compared with Lipofectamine/DNA complexes, an E3/DNA complex was less toxic and induced a comparable cellular level of plasmid DNA and expression levels of both mRNA and protein for IL-18. After injecting E3/DNA complexes into mice, the distribution of plasmid DNA was the highest in the lung and the liver. Especially, the administration of E3/DNA complexes induced a more rapid and prolonged distribution of plasmid DNA encoding IL-18 into the lung than that of Lipofectamine/DNA ones. These data demonstrated that cationic emulsion E3 containing castor oil could be useful for a delivery of IL-18 gene targeting the lung as well as the liver without an additional homing device, implying a potential IL-18 delivery system for the treatment of lung cancer.

Original languageEnglish
Pages (from-to)19-28
Number of pages10
JournalJournal of Drug Targeting
Volume17
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

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Interleukin-18
Emulsions
Lung Neoplasms
DNA
Genes
Plasmids
Lung
Interleukin-16
Castor Oil
Propane
Messenger RNA
Gene Targeting
Polysorbates
Poisons
Liver
Particle Size
Melanoma
Oils
Proteins
Neoplasm Metastasis

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Kang, Hyun Suk ; Jin, Shun Ji ; Myung, Chang Seon ; Hwang, Sung Joo ; Park, Jeong Sook. / Delivery of interleukin-18 gene to lung cancer cells using cationic emulsion. In: Journal of Drug Targeting. 2009 ; Vol. 17, No. 1. pp. 19-28.
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Delivery of interleukin-18 gene to lung cancer cells using cationic emulsion. / Kang, Hyun Suk; Jin, Shun Ji; Myung, Chang Seon; Hwang, Sung Joo; Park, Jeong Sook.

In: Journal of Drug Targeting, Vol. 17, No. 1, 01.01.2009, p. 19-28.

Research output: Contribution to journalArticle

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