Delta neutrophil index discriminates true bacteremia from blood culture contamination

Chang Hoon Lee, Juwon Kim, Yongjung Park, Yon Chul Park, Yoonjung Kim, Kap Jun Yoon, Young Uh, Kyunga Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Delta neutrophil index (DNI) has been reported to be useful in the diagnosis of sepsis. We evaluated the role of DNI for differentiating true bacteremia from blood contamination and compared the DNI value with previously validated markers such as procalcitonin (PCT) and C-reactive protein (CRP). Methods: The blood culture positive group was subdivided into true bacteremia (n = 199) and contamination (n = 158). The blood cultures were incubated in the BacT/Alert 3D (bioMérieux, Marcyl'Etoile, France) and BACTEC FX (Becton Dickinson, Sparks, MD, USA) systems for 5. days. Data of complete blood cell count were collected from an automatic cell analyzer (ADVIA2120 Hematology System, Siemens Healthcare Diagnostics) to calculate DNI. Results: Concentrations for DNI, PCT, and CRP were significantly higher in the true bacteremia group. When the gram-positive and gram-negative infections were compared among true bacteremia, only PCT was increased significantly in GNB bacteremia. DNI levels were well correlated with PCT (r = 0.564, P< 0.0001) and CRP (r = 0.344, P<. 0.001) using the Spearman test among the culture positive bacteremia. The area under the ROC curve was 0.75 for PCT, 0.69 for CRP, and 0.69 for DNI. Conclusions: We demonstrated the usefulness of DNI in differentiating true bacteremia from contamination in patients with a positive blood culture.

Original languageEnglish
Pages (from-to)11-14
Number of pages4
JournalClinica Chimica Acta
Volume427
DOIs
Publication statusPublished - 2014 Jan 1

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Calcitonin
Bacteremia
Neutrophils
Contamination
Blood
C-Reactive Protein
Blood Cell Count
Electric sparks
Cells
Blood Culture
Hematology
ROC Curve
France
Area Under Curve
Sepsis
Delivery of Health Care
Infection

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Lee, Chang Hoon ; Kim, Juwon ; Park, Yongjung ; Park, Yon Chul ; Kim, Yoonjung ; Yoon, Kap Jun ; Uh, Young ; Lee, Kyunga. / Delta neutrophil index discriminates true bacteremia from blood culture contamination. In: Clinica Chimica Acta. 2014 ; Vol. 427. pp. 11-14.
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abstract = "Background: Delta neutrophil index (DNI) has been reported to be useful in the diagnosis of sepsis. We evaluated the role of DNI for differentiating true bacteremia from blood contamination and compared the DNI value with previously validated markers such as procalcitonin (PCT) and C-reactive protein (CRP). Methods: The blood culture positive group was subdivided into true bacteremia (n = 199) and contamination (n = 158). The blood cultures were incubated in the BacT/Alert 3D (bioM{\'e}rieux, Marcyl'Etoile, France) and BACTEC FX (Becton Dickinson, Sparks, MD, USA) systems for 5. days. Data of complete blood cell count were collected from an automatic cell analyzer (ADVIA2120 Hematology System, Siemens Healthcare Diagnostics) to calculate DNI. Results: Concentrations for DNI, PCT, and CRP were significantly higher in the true bacteremia group. When the gram-positive and gram-negative infections were compared among true bacteremia, only PCT was increased significantly in GNB bacteremia. DNI levels were well correlated with PCT (r = 0.564, P< 0.0001) and CRP (r = 0.344, P<. 0.001) using the Spearman test among the culture positive bacteremia. The area under the ROC curve was 0.75 for PCT, 0.69 for CRP, and 0.69 for DNI. Conclusions: We demonstrated the usefulness of DNI in differentiating true bacteremia from contamination in patients with a positive blood culture.",
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Delta neutrophil index discriminates true bacteremia from blood culture contamination. / Lee, Chang Hoon; Kim, Juwon; Park, Yongjung; Park, Yon Chul; Kim, Yoonjung; Yoon, Kap Jun; Uh, Young; Lee, Kyunga.

In: Clinica Chimica Acta, Vol. 427, 01.01.2014, p. 11-14.

Research output: Contribution to journalArticle

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AU - Lee, Chang Hoon

AU - Kim, Juwon

AU - Park, Yongjung

AU - Park, Yon Chul

AU - Kim, Yoonjung

AU - Yoon, Kap Jun

AU - Uh, Young

AU - Lee, Kyunga

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N2 - Background: Delta neutrophil index (DNI) has been reported to be useful in the diagnosis of sepsis. We evaluated the role of DNI for differentiating true bacteremia from blood contamination and compared the DNI value with previously validated markers such as procalcitonin (PCT) and C-reactive protein (CRP). Methods: The blood culture positive group was subdivided into true bacteremia (n = 199) and contamination (n = 158). The blood cultures were incubated in the BacT/Alert 3D (bioMérieux, Marcyl'Etoile, France) and BACTEC FX (Becton Dickinson, Sparks, MD, USA) systems for 5. days. Data of complete blood cell count were collected from an automatic cell analyzer (ADVIA2120 Hematology System, Siemens Healthcare Diagnostics) to calculate DNI. Results: Concentrations for DNI, PCT, and CRP were significantly higher in the true bacteremia group. When the gram-positive and gram-negative infections were compared among true bacteremia, only PCT was increased significantly in GNB bacteremia. DNI levels were well correlated with PCT (r = 0.564, P< 0.0001) and CRP (r = 0.344, P<. 0.001) using the Spearman test among the culture positive bacteremia. The area under the ROC curve was 0.75 for PCT, 0.69 for CRP, and 0.69 for DNI. Conclusions: We demonstrated the usefulness of DNI in differentiating true bacteremia from contamination in patients with a positive blood culture.

AB - Background: Delta neutrophil index (DNI) has been reported to be useful in the diagnosis of sepsis. We evaluated the role of DNI for differentiating true bacteremia from blood contamination and compared the DNI value with previously validated markers such as procalcitonin (PCT) and C-reactive protein (CRP). Methods: The blood culture positive group was subdivided into true bacteremia (n = 199) and contamination (n = 158). The blood cultures were incubated in the BacT/Alert 3D (bioMérieux, Marcyl'Etoile, France) and BACTEC FX (Becton Dickinson, Sparks, MD, USA) systems for 5. days. Data of complete blood cell count were collected from an automatic cell analyzer (ADVIA2120 Hematology System, Siemens Healthcare Diagnostics) to calculate DNI. Results: Concentrations for DNI, PCT, and CRP were significantly higher in the true bacteremia group. When the gram-positive and gram-negative infections were compared among true bacteremia, only PCT was increased significantly in GNB bacteremia. DNI levels were well correlated with PCT (r = 0.564, P< 0.0001) and CRP (r = 0.344, P<. 0.001) using the Spearman test among the culture positive bacteremia. The area under the ROC curve was 0.75 for PCT, 0.69 for CRP, and 0.69 for DNI. Conclusions: We demonstrated the usefulness of DNI in differentiating true bacteremia from contamination in patients with a positive blood culture.

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