Dendrimer generation effects on photodynamic efficacy of dendrimer porphyrins and dendrimer-loaded supramolecular nanocarriers

Yuan Li, Woo Dong Jang, Nobuhiro Nishiyama, Akihiro Kishimura, Satoko Kawauchi, Yuji Morimoto, Sayaka Miake, Takashi Yamashita, Makoto Kikuchi, Takuzo Aida, Kazunori Kataoka

Research output: Contribution to journalArticle

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Abstract

A series of poly(benzyl ether) dendrimer porphyrins (DPs) (Gn = n-generation dendrimer, n = 1-3) was examined as potential photosensitizers for photodynamic therapy (PDT). Polyion complexes (PICs) between the DPs and poly(ethylene glycol)-block-poly(L-lysine) (PEG-b-PLL) were formed via an electrostatic interaction between the positively charged poly(L-lysine) (PLL) segment and negatively charged periphery of the DPs. Dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM) showed that G3 formed a core-shell-type nanocarrier micelle, whereas G1 and G2 formed irregular-shaped nanoparticles with a relatively high polydispersity. The photophysical properties of the DP-loaded PIC nanocarriers strongly depend on the generation of the DPs. In the case of G1 and G2, their fluorescence lifetime and oxygen consumption ability were significantly reduced by the formation of the PIC nanocarriers, whereas the G3-loaded PIC nanocarrier exhibited almost comparable fluorescence lifetimes and oxygen consumption abilities to the free G3. The incorporation of DPs into PIC nanocarriers resulted in an appreciable increase in the cellular uptake, yet inversely correlated with the generation. Alternatively, the photocytotoxicity of the DPs within the nanocarriers increased with an increase in the generation despite a decrease in the cellular uptake. By correlating the effects of the uptake amount with the photocytotoxicity, the PIC nanocarriers showed remarkable enhancement of the PDT efficacy dependent on the generation of DPs.

Original languageEnglish
Pages (from-to)5557-5562
Number of pages6
JournalChemistry of Materials
Volume19
Issue number23
DOIs
Publication statusPublished - 2007 Nov 13

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Dendrimers
Porphyrins
Photodynamic therapy
Lysine
Fluorescence
Oxygen
Photosensitizing Agents
Photosensitizers
Polydispersity
Micelles
Dynamic light scattering
Phase locked loops
Coulomb interactions
Ether
Polyethylene glycols
Ethers
Nanoparticles
Transmission electron microscopy

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

Cite this

Li, Yuan ; Jang, Woo Dong ; Nishiyama, Nobuhiro ; Kishimura, Akihiro ; Kawauchi, Satoko ; Morimoto, Yuji ; Miake, Sayaka ; Yamashita, Takashi ; Kikuchi, Makoto ; Aida, Takuzo ; Kataoka, Kazunori. / Dendrimer generation effects on photodynamic efficacy of dendrimer porphyrins and dendrimer-loaded supramolecular nanocarriers. In: Chemistry of Materials. 2007 ; Vol. 19, No. 23. pp. 5557-5562.
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title = "Dendrimer generation effects on photodynamic efficacy of dendrimer porphyrins and dendrimer-loaded supramolecular nanocarriers",
abstract = "A series of poly(benzyl ether) dendrimer porphyrins (DPs) (Gn = n-generation dendrimer, n = 1-3) was examined as potential photosensitizers for photodynamic therapy (PDT). Polyion complexes (PICs) between the DPs and poly(ethylene glycol)-block-poly(L-lysine) (PEG-b-PLL) were formed via an electrostatic interaction between the positively charged poly(L-lysine) (PLL) segment and negatively charged periphery of the DPs. Dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM) showed that G3 formed a core-shell-type nanocarrier micelle, whereas G1 and G2 formed irregular-shaped nanoparticles with a relatively high polydispersity. The photophysical properties of the DP-loaded PIC nanocarriers strongly depend on the generation of the DPs. In the case of G1 and G2, their fluorescence lifetime and oxygen consumption ability were significantly reduced by the formation of the PIC nanocarriers, whereas the G3-loaded PIC nanocarrier exhibited almost comparable fluorescence lifetimes and oxygen consumption abilities to the free G3. The incorporation of DPs into PIC nanocarriers resulted in an appreciable increase in the cellular uptake, yet inversely correlated with the generation. Alternatively, the photocytotoxicity of the DPs within the nanocarriers increased with an increase in the generation despite a decrease in the cellular uptake. By correlating the effects of the uptake amount with the photocytotoxicity, the PIC nanocarriers showed remarkable enhancement of the PDT efficacy dependent on the generation of DPs.",
author = "Yuan Li and Jang, {Woo Dong} and Nobuhiro Nishiyama and Akihiro Kishimura and Satoko Kawauchi and Yuji Morimoto and Sayaka Miake and Takashi Yamashita and Makoto Kikuchi and Takuzo Aida and Kazunori Kataoka",
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Li, Y, Jang, WD, Nishiyama, N, Kishimura, A, Kawauchi, S, Morimoto, Y, Miake, S, Yamashita, T, Kikuchi, M, Aida, T & Kataoka, K 2007, 'Dendrimer generation effects on photodynamic efficacy of dendrimer porphyrins and dendrimer-loaded supramolecular nanocarriers', Chemistry of Materials, vol. 19, no. 23, pp. 5557-5562. https://doi.org/10.1021/cm071451m

Dendrimer generation effects on photodynamic efficacy of dendrimer porphyrins and dendrimer-loaded supramolecular nanocarriers. / Li, Yuan; Jang, Woo Dong; Nishiyama, Nobuhiro; Kishimura, Akihiro; Kawauchi, Satoko; Morimoto, Yuji; Miake, Sayaka; Yamashita, Takashi; Kikuchi, Makoto; Aida, Takuzo; Kataoka, Kazunori.

In: Chemistry of Materials, Vol. 19, No. 23, 13.11.2007, p. 5557-5562.

Research output: Contribution to journalArticle

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T1 - Dendrimer generation effects on photodynamic efficacy of dendrimer porphyrins and dendrimer-loaded supramolecular nanocarriers

AU - Li, Yuan

AU - Jang, Woo Dong

AU - Nishiyama, Nobuhiro

AU - Kishimura, Akihiro

AU - Kawauchi, Satoko

AU - Morimoto, Yuji

AU - Miake, Sayaka

AU - Yamashita, Takashi

AU - Kikuchi, Makoto

AU - Aida, Takuzo

AU - Kataoka, Kazunori

PY - 2007/11/13

Y1 - 2007/11/13

N2 - A series of poly(benzyl ether) dendrimer porphyrins (DPs) (Gn = n-generation dendrimer, n = 1-3) was examined as potential photosensitizers for photodynamic therapy (PDT). Polyion complexes (PICs) between the DPs and poly(ethylene glycol)-block-poly(L-lysine) (PEG-b-PLL) were formed via an electrostatic interaction between the positively charged poly(L-lysine) (PLL) segment and negatively charged periphery of the DPs. Dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM) showed that G3 formed a core-shell-type nanocarrier micelle, whereas G1 and G2 formed irregular-shaped nanoparticles with a relatively high polydispersity. The photophysical properties of the DP-loaded PIC nanocarriers strongly depend on the generation of the DPs. In the case of G1 and G2, their fluorescence lifetime and oxygen consumption ability were significantly reduced by the formation of the PIC nanocarriers, whereas the G3-loaded PIC nanocarrier exhibited almost comparable fluorescence lifetimes and oxygen consumption abilities to the free G3. The incorporation of DPs into PIC nanocarriers resulted in an appreciable increase in the cellular uptake, yet inversely correlated with the generation. Alternatively, the photocytotoxicity of the DPs within the nanocarriers increased with an increase in the generation despite a decrease in the cellular uptake. By correlating the effects of the uptake amount with the photocytotoxicity, the PIC nanocarriers showed remarkable enhancement of the PDT efficacy dependent on the generation of DPs.

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