Denitrification rates and community structure of denitrifying bacteria in newly constructed wetland

Keunyea Song, Seung Hoon Lee, Hojeong Kang

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Constructed wetlands are generally created for water quality amelioration using natural biogeochemical processes including denitrification. To have maximal denitrification rates in constructed wetlands, both optimal environmental conditions and activation of denitrifiers are required. In this study, we monitored temporal dynamics of the community structure of denitrifying bacteria by terminal restriction fragment length polymorphism (T-RFLP) in newly constructed wetlands over 2 growing seasons. We also measured denitrification rates and denitrifying enzyme activity (DEA) using an acetylene blocking method. Community structure of denitrifying bacteria differed between 2 years, and dominant T-RFs changed over the period. In addition, the composition of denitrifying bacteria became simplified in the second year. Along with changes in community structure of denitrifying bacteria, DEA increased substantially in the second year compared with that in the first year. This result suggests that several dominant and active denitrifiers may induce higher potential for denitrification as wetlands became stabilized. However, actual denitrification rates did not exhibit an annual difference, but were more strongly influenced by environmental conditions such as temperature, pH, and DOC concentrations. Overall results of this study indicate that active denitrifiers may dominate and denitrification potentials increase as a newly constructed wetland becomes stabilized, but actual denitrification rates are mainly constrained by environmental conditions such as temperature, pH and carbon availability.

Original languageEnglish
Pages (from-to)24-29
Number of pages6
JournalEuropean Journal of Soil Biology
Volume47
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

Fingerprint

denitrifying bacteria
Denitrification
constructed wetlands
Wetlands
constructed wetland
denitrification
community structure
Bacteria
bacterium
environmental conditions
environmental factors
enzyme activity
Acetylene
acetylene
Temperature
Water Quality
Enzymes
rate
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Soil Science
  • Insect Science

Cite this

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abstract = "Constructed wetlands are generally created for water quality amelioration using natural biogeochemical processes including denitrification. To have maximal denitrification rates in constructed wetlands, both optimal environmental conditions and activation of denitrifiers are required. In this study, we monitored temporal dynamics of the community structure of denitrifying bacteria by terminal restriction fragment length polymorphism (T-RFLP) in newly constructed wetlands over 2 growing seasons. We also measured denitrification rates and denitrifying enzyme activity (DEA) using an acetylene blocking method. Community structure of denitrifying bacteria differed between 2 years, and dominant T-RFs changed over the period. In addition, the composition of denitrifying bacteria became simplified in the second year. Along with changes in community structure of denitrifying bacteria, DEA increased substantially in the second year compared with that in the first year. This result suggests that several dominant and active denitrifiers may induce higher potential for denitrification as wetlands became stabilized. However, actual denitrification rates did not exhibit an annual difference, but were more strongly influenced by environmental conditions such as temperature, pH, and DOC concentrations. Overall results of this study indicate that active denitrifiers may dominate and denitrification potentials increase as a newly constructed wetland becomes stabilized, but actual denitrification rates are mainly constrained by environmental conditions such as temperature, pH and carbon availability.",
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Denitrification rates and community structure of denitrifying bacteria in newly constructed wetland. / Song, Keunyea; Lee, Seung Hoon; Kang, Hojeong.

In: European Journal of Soil Biology, Vol. 47, No. 1, 01.01.2011, p. 24-29.

Research output: Contribution to journalArticle

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