Dental pulp of the third molar: A new source of pluripotent-like stem cells

Maher Atari, Carlos Gil-Recio, Marc Fabregat, Dani García-Fernández, Miguel Barajas, Miguel A. Carrasco, Han Sung Jung, F. Hernández Alfaro, Nuria Casals, Felipe Prosper, Eduard Ferrés-Padró, Luis Giner

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Dental pulp is particularly interesting in regenerative medicine because of the accessibility and differentiation potential of the tissue. Dental pulp has an early developmental origin with multi-lineage differentiation potential as a result of its development during childhood and adolescence. However, no study has previously identified the presence of stem cell populations with embryonic-like phenotypes in human dental pulp from the third molar. In the present work, we describe a new population of dental pulp pluripotent-like stem cells (DPPSCs) that were isolated by culture in medium containing LIF, EGF and PDGF. These cells are SSEA4+, OCT3/4+, NANOG+, SOX2+, LIN28+, CD13+, CD105+, CD342, CD452, CD90+, CD29+, CD73+, STRO1+ and CD1462, and they show genetic stability in vitro based on genomic analysis with a newly described CGH technique. Interestingly, DPPSCs were able to form both embryoid-body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ layers when injected in nude mice. We examined the capacity of DPPSCs to differentiate in vitro into tissues that have similar characteristics to mesoderm, endoderm and ectoderm layers in both 2D and 3D cultures. We performed a comparative RT-PCR analysis of GATA4, GATA6, MIXL1, NANOG, OCT3/4, SOX1 and SOX2 to determine the degree of similarity between DPPSCs, EBs and human induced pluripotent stem cells (hIPSCs). Our analysis revealed that DPPSCs, hIPSC and EBs have the same gene expression profile. Because DPPSCs can be derived from healthy human molars from patients of different sexes and ages, they represent an easily accessible source of stem cells, which opens a range of new possibilities for regenerative medicine.

Original languageEnglish
Pages (from-to)3343-3356
Number of pages14
JournalJournal of cell science
Volume125
Issue number14
DOIs
Publication statusPublished - 2012 Jul 15

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Dental Pulp
Pluripotent Stem Cells
Third Molar
Induced Pluripotent Stem Cells
Regenerative Medicine
Stem Cells
Embryoid Bodies
Germ Layers
Endoderm
Ectoderm
Teratoma
Mesoderm
Transcriptome
Epidermal Growth Factor
Nude Mice
Population
Culture Media
Phenotype
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Atari, M., Gil-Recio, C., Fabregat, M., García-Fernández, D., Barajas, M., Carrasco, M. A., ... Giner, L. (2012). Dental pulp of the third molar: A new source of pluripotent-like stem cells. Journal of cell science, 125(14), 3343-3356. https://doi.org/10.1242/jcs.096537
Atari, Maher ; Gil-Recio, Carlos ; Fabregat, Marc ; García-Fernández, Dani ; Barajas, Miguel ; Carrasco, Miguel A. ; Jung, Han Sung ; Alfaro, F. Hernández ; Casals, Nuria ; Prosper, Felipe ; Ferrés-Padró, Eduard ; Giner, Luis. / Dental pulp of the third molar : A new source of pluripotent-like stem cells. In: Journal of cell science. 2012 ; Vol. 125, No. 14. pp. 3343-3356.
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Atari, M, Gil-Recio, C, Fabregat, M, García-Fernández, D, Barajas, M, Carrasco, MA, Jung, HS, Alfaro, FH, Casals, N, Prosper, F, Ferrés-Padró, E & Giner, L 2012, 'Dental pulp of the third molar: A new source of pluripotent-like stem cells', Journal of cell science, vol. 125, no. 14, pp. 3343-3356. https://doi.org/10.1242/jcs.096537

Dental pulp of the third molar : A new source of pluripotent-like stem cells. / Atari, Maher; Gil-Recio, Carlos; Fabregat, Marc; García-Fernández, Dani; Barajas, Miguel; Carrasco, Miguel A.; Jung, Han Sung; Alfaro, F. Hernández; Casals, Nuria; Prosper, Felipe; Ferrés-Padró, Eduard; Giner, Luis.

In: Journal of cell science, Vol. 125, No. 14, 15.07.2012, p. 3343-3356.

Research output: Contribution to journalArticle

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AU - Prosper, Felipe

AU - Ferrés-Padró, Eduard

AU - Giner, Luis

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N2 - Dental pulp is particularly interesting in regenerative medicine because of the accessibility and differentiation potential of the tissue. Dental pulp has an early developmental origin with multi-lineage differentiation potential as a result of its development during childhood and adolescence. However, no study has previously identified the presence of stem cell populations with embryonic-like phenotypes in human dental pulp from the third molar. In the present work, we describe a new population of dental pulp pluripotent-like stem cells (DPPSCs) that were isolated by culture in medium containing LIF, EGF and PDGF. These cells are SSEA4+, OCT3/4+, NANOG+, SOX2+, LIN28+, CD13+, CD105+, CD342, CD452, CD90+, CD29+, CD73+, STRO1+ and CD1462, and they show genetic stability in vitro based on genomic analysis with a newly described CGH technique. Interestingly, DPPSCs were able to form both embryoid-body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ layers when injected in nude mice. We examined the capacity of DPPSCs to differentiate in vitro into tissues that have similar characteristics to mesoderm, endoderm and ectoderm layers in both 2D and 3D cultures. We performed a comparative RT-PCR analysis of GATA4, GATA6, MIXL1, NANOG, OCT3/4, SOX1 and SOX2 to determine the degree of similarity between DPPSCs, EBs and human induced pluripotent stem cells (hIPSCs). Our analysis revealed that DPPSCs, hIPSC and EBs have the same gene expression profile. Because DPPSCs can be derived from healthy human molars from patients of different sexes and ages, they represent an easily accessible source of stem cells, which opens a range of new possibilities for regenerative medicine.

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Atari M, Gil-Recio C, Fabregat M, García-Fernández D, Barajas M, Carrasco MA et al. Dental pulp of the third molar: A new source of pluripotent-like stem cells. Journal of cell science. 2012 Jul 15;125(14):3343-3356. https://doi.org/10.1242/jcs.096537