Depigmentation of melanocytes by isopanduratin A and 4-hydroxypanduratin A isolated from Kaempferia pandurata ROXB

Ji Hoon Yoon, Jae Seok Shim, Yumi Cho, Nam In Baek, Chan Woo Lee, Han Sung Kim, Jae-Kwan Hwang

Research output: Contribution to journalArticle

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Abstract

This study was carried out to investigate the in vitro effects of isopanduratin A and 4-hydroxypanduratin A isolated from Kaempferia pandurata ROXB. on melanin biosynthesis and tyrosinase activity. Two chalcone compounds, isopanduratin A and 4-hydroxypanduratin A, were isolated from the ethyl acetate fraction of ethanol extract as the active principles. Compared with phenylthiourea (IC50=34.3 μM) as a positive control, the depigmentation IC50 values for isopanduratin A and 4-hydroxypanduratin A were 10.6 μM and 23.2 μM, respectively. The compounds also significantly inhibited the activity of tyrosinase, the enzyme that converts DOPA (3,4-dihydroxyphenylalanine) to dopachrome in the biosynthetic process of melanin. The IC50 values of isopanduratin A and 4-hydroxypanduratin A for tyrosinase were 10.5 μM and >30 μM, respectively, while that of phenylthiourea was 47.6 μM. The tyrosinase protein level was also significantly decreased by isopanduratin A and 4-hydroxypanduratin A. The results indicate that isopanduratin A and 4-hydroxypanduratin A isolated from K. pandurata ROXB. are promising compounds that could be useful for treating hyperpigmentation as skin-whitening agents.

Original languageEnglish
Pages (from-to)2141-2145
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume30
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

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Zingiberaceae
Melanocytes
Monophenol Monooxygenase
Phenylthiourea
Inhibitory Concentration 50
Melanins
Skin Lightening Preparations
Chalcone
Hyperpigmentation
Dihydroxyphenylalanine
4-hydroxypanduratin A
isopanduratin A
Ethanol
Enzymes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Yoon, Ji Hoon ; Shim, Jae Seok ; Cho, Yumi ; Baek, Nam In ; Lee, Chan Woo ; Kim, Han Sung ; Hwang, Jae-Kwan. / Depigmentation of melanocytes by isopanduratin A and 4-hydroxypanduratin A isolated from Kaempferia pandurata ROXB. In: Biological and Pharmaceutical Bulletin. 2007 ; Vol. 30, No. 11. pp. 2141-2145.
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abstract = "This study was carried out to investigate the in vitro effects of isopanduratin A and 4-hydroxypanduratin A isolated from Kaempferia pandurata ROXB. on melanin biosynthesis and tyrosinase activity. Two chalcone compounds, isopanduratin A and 4-hydroxypanduratin A, were isolated from the ethyl acetate fraction of ethanol extract as the active principles. Compared with phenylthiourea (IC50=34.3 μM) as a positive control, the depigmentation IC50 values for isopanduratin A and 4-hydroxypanduratin A were 10.6 μM and 23.2 μM, respectively. The compounds also significantly inhibited the activity of tyrosinase, the enzyme that converts DOPA (3,4-dihydroxyphenylalanine) to dopachrome in the biosynthetic process of melanin. The IC50 values of isopanduratin A and 4-hydroxypanduratin A for tyrosinase were 10.5 μM and >30 μM, respectively, while that of phenylthiourea was 47.6 μM. The tyrosinase protein level was also significantly decreased by isopanduratin A and 4-hydroxypanduratin A. The results indicate that isopanduratin A and 4-hydroxypanduratin A isolated from K. pandurata ROXB. are promising compounds that could be useful for treating hyperpigmentation as skin-whitening agents.",
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Depigmentation of melanocytes by isopanduratin A and 4-hydroxypanduratin A isolated from Kaempferia pandurata ROXB. / Yoon, Ji Hoon; Shim, Jae Seok; Cho, Yumi; Baek, Nam In; Lee, Chan Woo; Kim, Han Sung; Hwang, Jae-Kwan.

In: Biological and Pharmaceutical Bulletin, Vol. 30, No. 11, 01.11.2007, p. 2141-2145.

Research output: Contribution to journalArticle

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T1 - Depigmentation of melanocytes by isopanduratin A and 4-hydroxypanduratin A isolated from Kaempferia pandurata ROXB

AU - Yoon, Ji Hoon

AU - Shim, Jae Seok

AU - Cho, Yumi

AU - Baek, Nam In

AU - Lee, Chan Woo

AU - Kim, Han Sung

AU - Hwang, Jae-Kwan

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