Depletion of mitochondrial DNA alters glucose metabolism in SK-Hep1 cells

Kyusang Park, Kyung Jay Nam, Jun Woo Kim, Youn Bok Lee, Chang Yeop Han, June Key Jeong, Hong Kyu Lee, Youngmi Kim Pak

Research output: Contribution to journalArticle

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Abstract

Maternally inherited mitochondrial DNA (mtDNA) has been suggested to be a genetic factor for diabetes. Reports have shown a decrease of mtDNA content in tissues of diabetic patients. We investigated the effects of mtDNA depletion on glucose metabolism by use of ρ0 SK-Hep1 human hepatoma cells, whose mtDNA was depleted by long-term exposure to ethidium bromide. The ρ0 cells failed to hyperpolarize mitochondrial membrane potential in response to glucose stimulation. Intracellular ATP content, glucose-stimulated ATP production, glucose uptake, steady-state mRNA and protein levels of glucose transporters, and cellular activities of glucose-metabolizing enzymes were decreased in ρ0 cells compared with parental ρ+ cells. Our results suggest that the quantitative reduction of mtDNA may suppress the expression of nuclear DNA-encoded glucose transporters and enzymes of glucose metabolism. Thus this may lead to diabetic status, such as decreased ATP production and glucose utilization.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume280
Issue number6 43-6
Publication statusPublished - 2001 Jul 3

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Mitochondrial DNA
Glucose
Facilitative Glucose Transport Proteins
Adenosine Triphosphate
Ethidium
Mitochondrial Membrane Potential
Enzymes
Hepatocellular Carcinoma
Messenger RNA
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Park, K., Nam, K. J., Kim, J. W., Lee, Y. B., Han, C. Y., Jeong, J. K., ... Pak, Y. K. (2001). Depletion of mitochondrial DNA alters glucose metabolism in SK-Hep1 cells. American Journal of Physiology - Endocrinology and Metabolism, 280(6 43-6).
Park, Kyusang ; Nam, Kyung Jay ; Kim, Jun Woo ; Lee, Youn Bok ; Han, Chang Yeop ; Jeong, June Key ; Lee, Hong Kyu ; Pak, Youngmi Kim. / Depletion of mitochondrial DNA alters glucose metabolism in SK-Hep1 cells. In: American Journal of Physiology - Endocrinology and Metabolism. 2001 ; Vol. 280, No. 6 43-6.
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Park, K, Nam, KJ, Kim, JW, Lee, YB, Han, CY, Jeong, JK, Lee, HK & Pak, YK 2001, 'Depletion of mitochondrial DNA alters glucose metabolism in SK-Hep1 cells', American Journal of Physiology - Endocrinology and Metabolism, vol. 280, no. 6 43-6.

Depletion of mitochondrial DNA alters glucose metabolism in SK-Hep1 cells. / Park, Kyusang; Nam, Kyung Jay; Kim, Jun Woo; Lee, Youn Bok; Han, Chang Yeop; Jeong, June Key; Lee, Hong Kyu; Pak, Youngmi Kim.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 280, No. 6 43-6, 03.07.2001.

Research output: Contribution to journalArticle

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