Depolymerization study of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering

Ji Hye Kwon, Euijin Hwang, Il Hwan Cho, Myeong Hee Moon

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Thermal depolymerization of ultrahigh-molecular-weight (UHMW) sodium hyaluronate (NaHA) was studied systematically by using frit-inlet asymmetrical flow field-flow fractionation/multiangle light scattering/differential refractive index (FI-AFlFFF/MALS/DRI). FI-AFlFFF was utilized for the size separation of NaHA samples which had been thermally degraded for varied treatment times, followed by light-scattering detection to determine MW and structural information of degraded NaHA products. Analysis of NaHA products showed time-dependent depolymerization of raw molecules into smaller-MW components, as well as unfolding of compact structures of UHMW NaHA. To determine whether the observed decrease in MW of sodium hyaluronate originated from the chain degradation of UHMW molecules or from dissociation of entangled complex particles that may have been formed by intermolecular association, narrow size fractions (1×107-6×107 and >6×107 MW) of NaHA molecules were collected during FlFFF separation and followed by thermal treatment. Subsequent FI-AFlFFF/MALS analysis of collected fractions after thermal treatment suggested that the ultrahigh-MW region (>107 Da) of NaHA is likely to result from supermolecular structures formed by aggregation of large molecules.

Original languageEnglish
Pages (from-to)519-525
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume395
Issue number2
DOIs
Publication statusPublished - 2009 Sep 1

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Field Flow Fractionation
Depolymerization
Hyaluronic Acid
Fractionation
Light scattering
Flow fields
Hot Temperature
Molecular Weight
Light
Molecules
Molecular weight
Refractometry
Size separation
Heat treatment
Inlet flow
Refractive index
Agglomeration
Association reactions
Degradation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

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abstract = "Thermal depolymerization of ultrahigh-molecular-weight (UHMW) sodium hyaluronate (NaHA) was studied systematically by using frit-inlet asymmetrical flow field-flow fractionation/multiangle light scattering/differential refractive index (FI-AFlFFF/MALS/DRI). FI-AFlFFF was utilized for the size separation of NaHA samples which had been thermally degraded for varied treatment times, followed by light-scattering detection to determine MW and structural information of degraded NaHA products. Analysis of NaHA products showed time-dependent depolymerization of raw molecules into smaller-MW components, as well as unfolding of compact structures of UHMW NaHA. To determine whether the observed decrease in MW of sodium hyaluronate originated from the chain degradation of UHMW molecules or from dissociation of entangled complex particles that may have been formed by intermolecular association, narrow size fractions (1×107-6×107 and >6×107 MW) of NaHA molecules were collected during FlFFF separation and followed by thermal treatment. Subsequent FI-AFlFFF/MALS analysis of collected fractions after thermal treatment suggested that the ultrahigh-MW region (>107 Da) of NaHA is likely to result from supermolecular structures formed by aggregation of large molecules.",
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Depolymerization study of sodium hyaluronate by flow field-flow fractionation/multiangle light scattering. / Kwon, Ji Hye; Hwang, Euijin; Cho, Il Hwan; Moon, Myeong Hee.

In: Analytical and Bioanalytical Chemistry, Vol. 395, No. 2, 01.09.2009, p. 519-525.

Research output: Contribution to journalArticle

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