A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth colocalization obtained from the OCT, we can penetrate 1.2-mm deep into strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (polystyrene) with a spatial offset. Our approach is capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter at depth within a 0.6-mm thick brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.
Bibliographical noteFunding Information:
UK Engineering and Physical Sciences Research Council, Grant/Award number: EP/J01771X/1, EP/M000869/1; a European Union FAMOS project, Grant/Award number: FP7 ICT, 317744; the RS MacDonald Charitable Trust
The authors thank the UK Engineering and Physical Sciences Research Council (EPSRC: EP/J01771X/1, EP/M000869/1), the European Union FAMOS project (FP7 ICT, 317 744) and the RS MacDonald Charitable Trust for funding.
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Physics and Astronomy(all)