DER containing two consecutive GTP-binding domains plays an essential role in chloroplast ribosomal RNA processing and ribosome biogenesis in higher plants

Young Jeon, Chang Sook Ahn, Hyun Ju Jung, Hunseung Kang, Guen Tae Park, Yeonhee Choi, Jihwan Hwang, Hyunsook Pai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This study investigated protein characteristics and physiological functions of DER (Double Era-like GTPase) of higher plants. Nicotiana benthamiana DER (NbDER) contained two tandemly repeated GTP-binding domains (GD) and a C-terminal domain (CTD) that was similar to the K-homology domain involved in RNA binding. Both GDs possessed GTPase activity and contributed to the maximum GTPase activity of NbDER. NbDER fused to green fluorescent protein was localized primarily to chloroplast nucleoids. Arabidopsis der null mutants exhibited an embryonic lethal phenotype, indicating an essential function of DER during plant embryogenesis. Virus-induced gene silencing of NbDER resulted in a leaf-yellowing phenotype caused by disrupted chloroplast biogenesis. NbDER was associated primarily with the chloroplast 50S ribosomal subunit in vivo, and both the CTD and the two GD contributed to the association. Recombinant proteins of NbDER and its CTD could bind to 23S and 16S ribosomal RNAs in vitro. Depletion of NbDER impaired processing of plastid-encoded ribosomal RNAs, resulting in accumulation of the precursor rRNAs in the chloroplasts. NbDER-deficient chloroplasts contained significantly reduced levels of mature 23S and 16S rRNAs and diverse mRNAs in the polysomal fractions, suggesting decreased translation in chloroplasts. These results suggest that DER is involved in chloroplast rRNA processing and ribosome biogenesis in higher plants.

Original languageEnglish
Pages (from-to)117-130
Number of pages14
JournalJournal of Experimental Botany
Volume65
Issue number1
DOIs
Publication statusPublished - 2014 Jan 27

Fingerprint

Chloroplast RNA
Ribosomal RNA
Nicotiana benthamiana
GTP Phosphohydrolases
guanosinetriphosphatase
Guanosine Triphosphate
ribosomes
Ribosomes
Chloroplasts
Tobacco
chloroplasts
ribosomal RNA
23S Ribosomal RNA
16S Ribosomal RNA
Phenotype
phenotype
Ribosome Subunits
Plastids
biogenesis
RNA Precursors

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Physiology

Cite this

Jeon, Young ; Ahn, Chang Sook ; Jung, Hyun Ju ; Kang, Hunseung ; Park, Guen Tae ; Choi, Yeonhee ; Hwang, Jihwan ; Pai, Hyunsook. / DER containing two consecutive GTP-binding domains plays an essential role in chloroplast ribosomal RNA processing and ribosome biogenesis in higher plants. In: Journal of Experimental Botany. 2014 ; Vol. 65, No. 1. pp. 117-130.
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DER containing two consecutive GTP-binding domains plays an essential role in chloroplast ribosomal RNA processing and ribosome biogenesis in higher plants. / Jeon, Young; Ahn, Chang Sook; Jung, Hyun Ju; Kang, Hunseung; Park, Guen Tae; Choi, Yeonhee; Hwang, Jihwan; Pai, Hyunsook.

In: Journal of Experimental Botany, Vol. 65, No. 1, 27.01.2014, p. 117-130.

Research output: Contribution to journalArticle

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AU - Jeon, Young

AU - Ahn, Chang Sook

AU - Jung, Hyun Ju

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AU - Pai, Hyunsook

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