Deregulated expression of microRNA-221 with the potential for prognostic biomarkers in surgically resected hepatocellular carcinoma

SunOch Yoon, Sung Min Chun, Eun Hee Han, Jene Choi, Se Jin Jang, Seung A. Koh, Shin Hwang, Eunsil Yu

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Aberrant expression of specific microRNAs in hepatocellular carcinomas has recently been reported. We examined expression patterns of 4 microRNAs (microRNA-221, microRNA-222, microRNA-21, and microRNA-155) to evaluate their potential as relevant biomarkers by quantitative real-time reverse transcriptase-polymerase chain reaction using formalin-fixed, paraffin-embedded tissues of 115 surgically resected hepatocellular carcinoma and paired nonneoplastic liver cases as well as 21 normal liver samples from cancer-free individuals. MicroRNA-221, microRNA-222, and microRNA-21 were differentially overexpressed in hepatocellular carcinoma compared with nonneoplastic and normal livers (P <.001). The mean fold changes in microRNA-221, microRNA-222, and microRNA-21(hepatocellular carcinoma to matched nonneoplastic liver) were 4.00, 4.44, and, 3.67, respectively. In addition, nonneoplastic liver tissues displayed higher levels of microRNA-221, microRNA-222, microRNA-21, and microRNA-155 than normal livers (P <.001, respectively). However, the overexpression of the 4 microRNAs showed no consistent relevance to the known prognostic clinicopathologic parameters. High expression of microRNA-221 in hepatocellular carcinomas was significantly related to shorter time to local recurrence (P <.001) and determined as an independent predictor for local recurrence (P =.001). The fold changes in microRNA-221 (hepatocellular carcinoma to matched nonneoplastic liver) less than 1 were more commonly detected in cases of distant metastases than those of disease-free and local recurrence (P =.009). The fold changes less than 1 were related to reduced metastasis-free survival (P =.006) and thus can be used as an independent predictor of distant metastasis after surgical resection (P =.027). Based on these results, we propose the possible role of microRNA-221, microRNA-222, microRNA-21, and microRNA-155 dysregulation in hepatocarcinogenesis and the potential of microRNA-221 dysregulation for predicting local recurrence and distant metastasis after curative surgery.

Original languageEnglish
Pages (from-to)1391-1400
Number of pages10
JournalHuman Pathology
Volume42
Issue number10
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

MicroRNAs
Hepatocellular Carcinoma
Biomarkers
Liver
Neoplasm Metastasis
Recurrence

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Yoon, SunOch ; Chun, Sung Min ; Han, Eun Hee ; Choi, Jene ; Jang, Se Jin ; Koh, Seung A. ; Hwang, Shin ; Yu, Eunsil. / Deregulated expression of microRNA-221 with the potential for prognostic biomarkers in surgically resected hepatocellular carcinoma. In: Human Pathology. 2011 ; Vol. 42, No. 10. pp. 1391-1400.
@article{58687ac790fa475e9d7520fac20ce367,
title = "Deregulated expression of microRNA-221 with the potential for prognostic biomarkers in surgically resected hepatocellular carcinoma",
abstract = "Aberrant expression of specific microRNAs in hepatocellular carcinomas has recently been reported. We examined expression patterns of 4 microRNAs (microRNA-221, microRNA-222, microRNA-21, and microRNA-155) to evaluate their potential as relevant biomarkers by quantitative real-time reverse transcriptase-polymerase chain reaction using formalin-fixed, paraffin-embedded tissues of 115 surgically resected hepatocellular carcinoma and paired nonneoplastic liver cases as well as 21 normal liver samples from cancer-free individuals. MicroRNA-221, microRNA-222, and microRNA-21 were differentially overexpressed in hepatocellular carcinoma compared with nonneoplastic and normal livers (P <.001). The mean fold changes in microRNA-221, microRNA-222, and microRNA-21(hepatocellular carcinoma to matched nonneoplastic liver) were 4.00, 4.44, and, 3.67, respectively. In addition, nonneoplastic liver tissues displayed higher levels of microRNA-221, microRNA-222, microRNA-21, and microRNA-155 than normal livers (P <.001, respectively). However, the overexpression of the 4 microRNAs showed no consistent relevance to the known prognostic clinicopathologic parameters. High expression of microRNA-221 in hepatocellular carcinomas was significantly related to shorter time to local recurrence (P <.001) and determined as an independent predictor for local recurrence (P =.001). The fold changes in microRNA-221 (hepatocellular carcinoma to matched nonneoplastic liver) less than 1 were more commonly detected in cases of distant metastases than those of disease-free and local recurrence (P =.009). The fold changes less than 1 were related to reduced metastasis-free survival (P =.006) and thus can be used as an independent predictor of distant metastasis after surgical resection (P =.027). Based on these results, we propose the possible role of microRNA-221, microRNA-222, microRNA-21, and microRNA-155 dysregulation in hepatocarcinogenesis and the potential of microRNA-221 dysregulation for predicting local recurrence and distant metastasis after curative surgery.",
author = "SunOch Yoon and Chun, {Sung Min} and Han, {Eun Hee} and Jene Choi and Jang, {Se Jin} and Koh, {Seung A.} and Shin Hwang and Eunsil Yu",
year = "2011",
month = "10",
day = "1",
doi = "10.1016/j.humpath.2010.12.010",
language = "English",
volume = "42",
pages = "1391--1400",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "10",

}

Deregulated expression of microRNA-221 with the potential for prognostic biomarkers in surgically resected hepatocellular carcinoma. / Yoon, SunOch; Chun, Sung Min; Han, Eun Hee; Choi, Jene; Jang, Se Jin; Koh, Seung A.; Hwang, Shin; Yu, Eunsil.

In: Human Pathology, Vol. 42, No. 10, 01.10.2011, p. 1391-1400.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Deregulated expression of microRNA-221 with the potential for prognostic biomarkers in surgically resected hepatocellular carcinoma

AU - Yoon, SunOch

AU - Chun, Sung Min

AU - Han, Eun Hee

AU - Choi, Jene

AU - Jang, Se Jin

AU - Koh, Seung A.

AU - Hwang, Shin

AU - Yu, Eunsil

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Aberrant expression of specific microRNAs in hepatocellular carcinomas has recently been reported. We examined expression patterns of 4 microRNAs (microRNA-221, microRNA-222, microRNA-21, and microRNA-155) to evaluate their potential as relevant biomarkers by quantitative real-time reverse transcriptase-polymerase chain reaction using formalin-fixed, paraffin-embedded tissues of 115 surgically resected hepatocellular carcinoma and paired nonneoplastic liver cases as well as 21 normal liver samples from cancer-free individuals. MicroRNA-221, microRNA-222, and microRNA-21 were differentially overexpressed in hepatocellular carcinoma compared with nonneoplastic and normal livers (P <.001). The mean fold changes in microRNA-221, microRNA-222, and microRNA-21(hepatocellular carcinoma to matched nonneoplastic liver) were 4.00, 4.44, and, 3.67, respectively. In addition, nonneoplastic liver tissues displayed higher levels of microRNA-221, microRNA-222, microRNA-21, and microRNA-155 than normal livers (P <.001, respectively). However, the overexpression of the 4 microRNAs showed no consistent relevance to the known prognostic clinicopathologic parameters. High expression of microRNA-221 in hepatocellular carcinomas was significantly related to shorter time to local recurrence (P <.001) and determined as an independent predictor for local recurrence (P =.001). The fold changes in microRNA-221 (hepatocellular carcinoma to matched nonneoplastic liver) less than 1 were more commonly detected in cases of distant metastases than those of disease-free and local recurrence (P =.009). The fold changes less than 1 were related to reduced metastasis-free survival (P =.006) and thus can be used as an independent predictor of distant metastasis after surgical resection (P =.027). Based on these results, we propose the possible role of microRNA-221, microRNA-222, microRNA-21, and microRNA-155 dysregulation in hepatocarcinogenesis and the potential of microRNA-221 dysregulation for predicting local recurrence and distant metastasis after curative surgery.

AB - Aberrant expression of specific microRNAs in hepatocellular carcinomas has recently been reported. We examined expression patterns of 4 microRNAs (microRNA-221, microRNA-222, microRNA-21, and microRNA-155) to evaluate their potential as relevant biomarkers by quantitative real-time reverse transcriptase-polymerase chain reaction using formalin-fixed, paraffin-embedded tissues of 115 surgically resected hepatocellular carcinoma and paired nonneoplastic liver cases as well as 21 normal liver samples from cancer-free individuals. MicroRNA-221, microRNA-222, and microRNA-21 were differentially overexpressed in hepatocellular carcinoma compared with nonneoplastic and normal livers (P <.001). The mean fold changes in microRNA-221, microRNA-222, and microRNA-21(hepatocellular carcinoma to matched nonneoplastic liver) were 4.00, 4.44, and, 3.67, respectively. In addition, nonneoplastic liver tissues displayed higher levels of microRNA-221, microRNA-222, microRNA-21, and microRNA-155 than normal livers (P <.001, respectively). However, the overexpression of the 4 microRNAs showed no consistent relevance to the known prognostic clinicopathologic parameters. High expression of microRNA-221 in hepatocellular carcinomas was significantly related to shorter time to local recurrence (P <.001) and determined as an independent predictor for local recurrence (P =.001). The fold changes in microRNA-221 (hepatocellular carcinoma to matched nonneoplastic liver) less than 1 were more commonly detected in cases of distant metastases than those of disease-free and local recurrence (P =.009). The fold changes less than 1 were related to reduced metastasis-free survival (P =.006) and thus can be used as an independent predictor of distant metastasis after surgical resection (P =.027). Based on these results, we propose the possible role of microRNA-221, microRNA-222, microRNA-21, and microRNA-155 dysregulation in hepatocarcinogenesis and the potential of microRNA-221 dysregulation for predicting local recurrence and distant metastasis after curative surgery.

UR - http://www.scopus.com/inward/record.url?scp=80053184413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053184413&partnerID=8YFLogxK

U2 - 10.1016/j.humpath.2010.12.010

DO - 10.1016/j.humpath.2010.12.010

M3 - Article

VL - 42

SP - 1391

EP - 1400

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 10

ER -