Design aspects of bright red emissive silver nanoclusters/DNA probes for MicroRNA detection

Pratik Shah, Andreas Rørvig-Lund, Samir Ben Chaabane, Peter Waaben Thulstrup, Henrik Grum Kjaergaard, Eduard Fron, Johan Hofkens, Seong Wook Yang, Tom Vosch

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.

Original languageEnglish
Pages (from-to)8803-8814
Number of pages12
JournalACS Nano
Volume6
Issue number10
DOIs
Publication statusPublished - 2012 Oct 23

Fingerprint

Nanoclusters
DNA Probes
nanoclusters
MicroRNAs
Silver
DNA
deoxyribonucleic acid
silver
Dimers
probes
RNA
DNA sequences
Nucleic Acids
Animals
Thermodynamic stability
Stabilization
Monomers
dimers
Nucleic acids
nucleic acids

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Shah, P., Rørvig-Lund, A., Chaabane, S. B., Thulstrup, P. W., Kjaergaard, H. G., Fron, E., ... Vosch, T. (2012). Design aspects of bright red emissive silver nanoclusters/DNA probes for MicroRNA detection. ACS Nano, 6(10), 8803-8814. https://doi.org/10.1021/nn302633q
Shah, Pratik ; Rørvig-Lund, Andreas ; Chaabane, Samir Ben ; Thulstrup, Peter Waaben ; Kjaergaard, Henrik Grum ; Fron, Eduard ; Hofkens, Johan ; Yang, Seong Wook ; Vosch, Tom. / Design aspects of bright red emissive silver nanoclusters/DNA probes for MicroRNA detection. In: ACS Nano. 2012 ; Vol. 6, No. 10. pp. 8803-8814.
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Shah, P, Rørvig-Lund, A, Chaabane, SB, Thulstrup, PW, Kjaergaard, HG, Fron, E, Hofkens, J, Yang, SW & Vosch, T 2012, 'Design aspects of bright red emissive silver nanoclusters/DNA probes for MicroRNA detection', ACS Nano, vol. 6, no. 10, pp. 8803-8814. https://doi.org/10.1021/nn302633q

Design aspects of bright red emissive silver nanoclusters/DNA probes for MicroRNA detection. / Shah, Pratik; Rørvig-Lund, Andreas; Chaabane, Samir Ben; Thulstrup, Peter Waaben; Kjaergaard, Henrik Grum; Fron, Eduard; Hofkens, Johan; Yang, Seong Wook; Vosch, Tom.

In: ACS Nano, Vol. 6, No. 10, 23.10.2012, p. 8803-8814.

Research output: Contribution to journalArticle

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AU - Kjaergaard, Henrik Grum

AU - Fron, Eduard

AU - Hofkens, Johan

AU - Yang, Seong Wook

AU - Vosch, Tom

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Shah P, Rørvig-Lund A, Chaabane SB, Thulstrup PW, Kjaergaard HG, Fron E et al. Design aspects of bright red emissive silver nanoclusters/DNA probes for MicroRNA detection. ACS Nano. 2012 Oct 23;6(10):8803-8814. https://doi.org/10.1021/nn302633q