To develop biocompatible non-viral gene carriers, poly(ethylene glycol)(PEG)-based block catiomers carrying the ethylene diamine moiety (PEG-P[Asp(DET)]) in the side chain were prepared through the aminolysis reaction of PEG-poly(ß-benzyl L-aspartate) (PEG-PBLA) with 50 eq. of diethylenetriamine (DET) in DMF. PEG-P[Asp(DET)] formed the polyplex micelles with the size of ∼100 nm and almost neutral ζ-potential, which are characterized by the structure of the packaged DNA surrounded by the PEG outer shell. Biological studies have revealed that the polyplex micelles show the efficient transfection and remarkably low cytotoxicity toward primary cells. Thus, the PEG-P[Asp(DET)] system might be a promising vector for in vivo topical gene therapy.
|Number of pages||2|
|Publication status||Published - 2005|
|Event||54th SPSJ Symposium on Macromolecules - Yamagata, Japan|
Duration: 2005 Sep 20 → 2005 Sep 22
|Other||54th SPSJ Symposium on Macromolecules|
|Period||05/9/20 → 05/9/22|
All Science Journal Classification (ASJC) codes