Design of biocompatible block catiomers directed to effective transfection with minimal toxicity

Nobuhiro Nisihiyama, Keiji Itaka, Shigeto Fukushima, Woo Dong Jang, Naoki Kanayama, Kanjiro Miyata, Shunsaku Asano, Ung Li Chung, Yuichi Yamasaki, Kazunori Kataoka

Research output: Contribution to conferencePaper

Abstract

To develop biocompatible non-viral gene carriers, poly(ethylene glycol)(PEG)-based block catiomers carrying the ethylene diamine moiety (PEG-P[Asp(DET)]) in the side chain were prepared through the aminolysis reaction of PEG-poly(ß-benzyl L-aspartate) (PEG-PBLA) with 50 eq. of diethylenetriamine (DET) in DMF. PEG-P[Asp(DET)] formed the polyplex micelles with the size of ∼100 nm and almost neutral ζ-potential, which are characterized by the structure of the packaged DNA surrounded by the PEG outer shell. Biological studies have revealed that the polyplex micelles show the efficient transfection and remarkably low cytotoxicity toward primary cells. Thus, the PEG-P[Asp(DET)] system might be a promising vector for in vivo topical gene therapy.

Original languageEnglish
Pages5108-5109
Number of pages2
Publication statusPublished - 2005
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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    Nisihiyama, N., Itaka, K., Fukushima, S., Jang, W. D., Kanayama, N., Miyata, K., Asano, S., Chung, U. L., Yamasaki, Y., & Kataoka, K. (2005). Design of biocompatible block catiomers directed to effective transfection with minimal toxicity. 5108-5109. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.