Design of Magnetically Labeled Cells (Mag-Cells) for in Vivo Control of Stem Cell Migration and Differentiation

Seokhwan Yun, Tae Hyun Shin, Jae-Hyun Lee, Mi Hyeon Cho, Il Sun Kim, Ji Wook Kim, Kwangsoo Jung, Il Shin Lee, Jinwoo Cheon, Kook In Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cell-based therapies are attractive for treating various degenerative disorders and cancer but delivering functional cells to the region of interest in vivo remains difficult. The problem is exacerbated in dense biological matrices such as solid tissues because these environments impose significant steric hindrances for cell movement. Here, we show that neural stem cells transfected with zinc-doped ferrite magnetic nanoparticles (ZnMNPs) can be pulled by an external magnet to migrate to the desired location in the brain. These magnetically labeled cells (Mag-Cells) can migrate because ZnMNPs generate sufficiently strong mechanical forces to overcome steric hindrances in the brain tissues. Once at the site of lesion, Mag-Cells show enhanced neuronal differentiation and greater secretion of neurotrophic factors than unlabeled control stem cells. Our study shows that ZnMNPs activate zinc-mediated Wnt signaling to facilitate neuronal differentiation. When implemented in a rodent brain stroke model, Mag-Cells led to significant recovery of locomotor performance in the impaired limbs of the animals. Our findings provide a simple magnetic method for controlling migration of stem cells with high therapeutic functions, offering a valuable tool for other cell-based therapies.

Original languageEnglish
Pages (from-to)838-845
Number of pages8
JournalNano letters
Volume18
Issue number2
DOIs
Publication statusPublished - 2018 Feb 14

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stem cells
Stem cells
Zinc
Ferrite
Nanoparticles
zinc
cells
brain
ferrites
Brain
Brain models
Tissue
nanoparticles
therapy
Nerve Growth Factors
Magnets
rodents
secretions
Animals
Cells

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Yun, Seokhwan ; Shin, Tae Hyun ; Lee, Jae-Hyun ; Cho, Mi Hyeon ; Kim, Il Sun ; Kim, Ji Wook ; Jung, Kwangsoo ; Lee, Il Shin ; Cheon, Jinwoo ; Park, Kook In. / Design of Magnetically Labeled Cells (Mag-Cells) for in Vivo Control of Stem Cell Migration and Differentiation. In: Nano letters. 2018 ; Vol. 18, No. 2. pp. 838-845.
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Design of Magnetically Labeled Cells (Mag-Cells) for in Vivo Control of Stem Cell Migration and Differentiation. / Yun, Seokhwan; Shin, Tae Hyun; Lee, Jae-Hyun; Cho, Mi Hyeon; Kim, Il Sun; Kim, Ji Wook; Jung, Kwangsoo; Lee, Il Shin; Cheon, Jinwoo; Park, Kook In.

In: Nano letters, Vol. 18, No. 2, 14.02.2018, p. 838-845.

Research output: Contribution to journalArticle

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