Design of nano-assemblies for the light-directed gene transfer [2]

Nobuhiro Nishiyama; Woo Dong Jang; Aya Akagi; Yuji Inoue; Yasuo Yanagi; Yasuhiro Tamaki; Kazunori Kataoka

Design of nano-assemblies for the light-directed gene transfer [2]

Nobuhiro Nishiyama, Woo Dong Jang, Aya Akagi, Yuji Inoue, Yasuo Yanagi, Yasuhiro Tamaki, Kazunori Kataoka

Department of Chemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.

Original language	English
Pages	2187
Number of pages	1
Publication status	Published - 2005
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Other

Other	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

All Science Journal Classification (ASJC) codes

Engineering(all)

Cite this

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title = "Design of nano-assemblies for the light-directed gene transfer [2]",

abstract = "Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.",

author = "Nobuhiro Nishiyama and Jang, {Woo Dong} and Aya Akagi and Yuji Inoue and Yasuo Yanagi and Yasuhiro Tamaki and Kazunori Kataoka",

year = "2005",

language = "English",

pages = "2187",

note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

}

TY - CONF

T1 - Design of nano-assemblies for the light-directed gene transfer [2]

AU - Nishiyama, Nobuhiro

AU - Jang, Woo Dong

AU - Akagi, Aya

AU - Inoue, Yuji

AU - Yanagi, Yasuo

AU - Tamaki, Yasuhiro

AU - Kataoka, Kazunori

PY - 2005

Y1 - 2005

N2 - Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.

AB - Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.

UR - http://www.scopus.com/inward/record.url?scp=33645643077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645643077&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645643077

SP - 2187

T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

Design of nano-assemblies for the light-directed gene transfer [2]

Abstract

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