Design of nano-assemblies for the light-directed gene transfer [2]

Nobuhiro Nishiyama, Woo Dong Jang, Aya Akagi, Yuji Inoue, Yasuo Yanagi, Yasuhiro Tamaki, Kazunori Kataoka

Research output: Contribution to conferencePaper

Abstract

Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

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Gene transfer
Photosensitizers
Dendrimers
Cytotoxicity
Peptides
DNA
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Nishiyama, N., Jang, W. D., Akagi, A., Inoue, Y., Yanagi, Y., Tamaki, Y., & Kataoka, K. (2005). Design of nano-assemblies for the light-directed gene transfer [2]. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.
Nishiyama, Nobuhiro ; Jang, Woo Dong ; Akagi, Aya ; Inoue, Yuji ; Yanagi, Yasuo ; Tamaki, Yasuhiro ; Kataoka, Kazunori. / Design of nano-assemblies for the light-directed gene transfer [2]. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.1 p.
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Nishiyama, N, Jang, WD, Akagi, A, Inoue, Y, Yanagi, Y, Tamaki, Y & Kataoka, K 2005, 'Design of nano-assemblies for the light-directed gene transfer [2]' Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 05/5/25 - 05/5/27, .

Design of nano-assemblies for the light-directed gene transfer [2]. / Nishiyama, Nobuhiro; Jang, Woo Dong; Akagi, Aya; Inoue, Yuji; Yanagi, Yasuo; Tamaki, Yasuhiro; Kataoka, Kazunori.

2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Research output: Contribution to conferencePaper

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T1 - Design of nano-assemblies for the light-directed gene transfer [2]

AU - Nishiyama, Nobuhiro

AU - Jang, Woo Dong

AU - Akagi, Aya

AU - Inoue, Yuji

AU - Yanagi, Yasuo

AU - Tamaki, Yasuhiro

AU - Kataoka, Kazunori

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.

AB - Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.

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Nishiyama N, Jang WD, Akagi A, Inoue Y, Yanagi Y, Tamaki Y et al. Design of nano-assemblies for the light-directed gene transfer [2]. 2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.