Design of nano-assemblies for the light-directed gene transfer [2]

Nobuhiro Nishiyama; Woo Dong Jang; Aya Akagi; Yuji Inoue; Yasuo Yanagi; Yasuhiro Tamaki; Kazunori Kataoka

Design of nano-assemblies for the light-directed gene transfer [2]

Nobuhiro Nishiyama, Woo Dong Jang, Aya Akagi, Yuji Inoue, Yasuo Yanagi, Yasuhiro Tamaki, Kazunori Kataoka

Department of Chemistry

Research output: Contribution to conference › Paper

Abstract

Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.

Original language	English
Pages	2187
Number of pages	1
Publication status	Published - 2005
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Other

Other	54th SPSJ Annual Meeting 2005
Country	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

All Science Journal Classification (ASJC) codes

Engineering(all)

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Nishiyama, N., Jang, W. D., Akagi, A., Inoue, Y., Yanagi, Y., Tamaki, Y., & Kataoka, K. (2005). Design of nano-assemblies for the light-directed gene transfer [2]. 2187. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

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T1 - Design of nano-assemblies for the light-directed gene transfer [2]

AU - Nishiyama, Nobuhiro

AU - Jang, Woo Dong

AU - Akagi, Aya

AU - Inoue, Yuji

AU - Yanagi, Yasuo

AU - Tamaki, Yasuhiro

AU - Kataoka, Kazunori

PY - 2005

Y1 - 2005

N2 - Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.

AB - Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.

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T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

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