Abstract
Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.
| Original language | English |
|---|---|
| Number of pages | 1 |
| Publication status | Published - 2005 Dec 1 |
| Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27 |
Other
| Other | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country | Japan |
| City | Yokohama |
| Period | 05/5/25 → 05/5/27 |
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All Science Journal Classification (ASJC) codes
- Engineering(all)
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Design of nano-assemblies for the light-directed gene transfer [2]. / Nishiyama, Nobuhiro; Jang, Woo Dong; Akagi, Aya; Inoue, Yuji; Yanagi, Yasuo; Tamaki, Yasuhiro; Kataoka, Kazunori.
2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.Research output: Contribution to conference › Paper
TY - CONF
T1 - Design of nano-assemblies for the light-directed gene transfer [2]
AU - Nishiyama, Nobuhiro
AU - Jang, Woo Dong
AU - Akagi, Aya
AU - Inoue, Yuji
AU - Yanagi, Yasuo
AU - Tamaki, Yasuhiro
AU - Kataoka, Kazunori
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.
AB - Novel ternary complexes formed from plasmid DNA, cationic peptides and anionic dendrimer photosensitizers were newly developed for the light-directed transfection. In this study, the atomic force microscopic (AFM) observation has revealed that the ternary complexes possessing optimal compositions had a spherical shape with the diameter of approximately 150nm and a narrow size distribution, and the in vitro biological studies have demonstrated that such ternary complexes showed the efficient light-induced transfection while showing little photocytotoxicity and cytotoxicity after a prolonged incubation. Thus, the ternary complexes are expected to be a promising non-viral vector for site-directed transfection in vivo.
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M3 - Paper
ER -