Destruction of Bacillus licheniformis spores by microwave irradiation

S. Y. Kim, S. J. Shin, C. H. Song, E. K. Jo, H. J. Kim, J. K. Park

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Aims: To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. Methods and Results: We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0·5 kW) was modified to output power at 2·0 kW, which allowed a shorter sterilization cycle. A 2·0 kW microwave treatment at the boiling temperature for 1 min did not kill all spores, but killed most spores. The spore inactivation rate was faster than that of boiling and 0·5 kW microwave oven. In contrast to boiling and 0·5 kW microwave treatments, the 2·0 kW microwave resulted in significant leakage of proteins and DNA from spores due to injury to the spore structure. TEM revealed that 2·0 kW microwave irradiation affected spore cortex hydrolysis and swelling, and ruptured the spore coat and inner membrane. Conclusions: These results suggest that 2·0 kW microwave irradiation ruptures the spore coat and inner membrane, and is significantly different from boiling. Significance and Impact of the Study: This study provides information on the sporicidal mechanisms of microwave irradiation on B. licheniformis spores.

Original languageEnglish
Pages (from-to)877-885
Number of pages9
JournalJournal of Applied Microbiology
Volume106
Issue number3
DOIs
Publication statusPublished - 2009 Mar 1

Fingerprint

Microwaves
Spores
Transmission Electron Microscopy
Bacillus licheniformis
Membranes
DNA
Rupture
Proteins
Hydrolysis
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Kim, S. Y. ; Shin, S. J. ; Song, C. H. ; Jo, E. K. ; Kim, H. J. ; Park, J. K. / Destruction of Bacillus licheniformis spores by microwave irradiation. In: Journal of Applied Microbiology. 2009 ; Vol. 106, No. 3. pp. 877-885.
@article{9d09392d195a4672a2870807fab869d6,
title = "Destruction of Bacillus licheniformis spores by microwave irradiation",
abstract = "Aims: To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. Methods and Results: We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0·5 kW) was modified to output power at 2·0 kW, which allowed a shorter sterilization cycle. A 2·0 kW microwave treatment at the boiling temperature for 1 min did not kill all spores, but killed most spores. The spore inactivation rate was faster than that of boiling and 0·5 kW microwave oven. In contrast to boiling and 0·5 kW microwave treatments, the 2·0 kW microwave resulted in significant leakage of proteins and DNA from spores due to injury to the spore structure. TEM revealed that 2·0 kW microwave irradiation affected spore cortex hydrolysis and swelling, and ruptured the spore coat and inner membrane. Conclusions: These results suggest that 2·0 kW microwave irradiation ruptures the spore coat and inner membrane, and is significantly different from boiling. Significance and Impact of the Study: This study provides information on the sporicidal mechanisms of microwave irradiation on B. licheniformis spores.",
author = "Kim, {S. Y.} and Shin, {S. J.} and Song, {C. H.} and Jo, {E. K.} and Kim, {H. J.} and Park, {J. K.}",
year = "2009",
month = "3",
day = "1",
doi = "10.1111/j.1365-2672.2008.04056.x",
language = "English",
volume = "106",
pages = "877--885",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "3",

}

Destruction of Bacillus licheniformis spores by microwave irradiation. / Kim, S. Y.; Shin, S. J.; Song, C. H.; Jo, E. K.; Kim, H. J.; Park, J. K.

In: Journal of Applied Microbiology, Vol. 106, No. 3, 01.03.2009, p. 877-885.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Destruction of Bacillus licheniformis spores by microwave irradiation

AU - Kim, S. Y.

AU - Shin, S. J.

AU - Song, C. H.

AU - Jo, E. K.

AU - Kim, H. J.

AU - Park, J. K.

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Aims: To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. Methods and Results: We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0·5 kW) was modified to output power at 2·0 kW, which allowed a shorter sterilization cycle. A 2·0 kW microwave treatment at the boiling temperature for 1 min did not kill all spores, but killed most spores. The spore inactivation rate was faster than that of boiling and 0·5 kW microwave oven. In contrast to boiling and 0·5 kW microwave treatments, the 2·0 kW microwave resulted in significant leakage of proteins and DNA from spores due to injury to the spore structure. TEM revealed that 2·0 kW microwave irradiation affected spore cortex hydrolysis and swelling, and ruptured the spore coat and inner membrane. Conclusions: These results suggest that 2·0 kW microwave irradiation ruptures the spore coat and inner membrane, and is significantly different from boiling. Significance and Impact of the Study: This study provides information on the sporicidal mechanisms of microwave irradiation on B. licheniformis spores.

AB - Aims: To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. Methods and Results: We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0·5 kW) was modified to output power at 2·0 kW, which allowed a shorter sterilization cycle. A 2·0 kW microwave treatment at the boiling temperature for 1 min did not kill all spores, but killed most spores. The spore inactivation rate was faster than that of boiling and 0·5 kW microwave oven. In contrast to boiling and 0·5 kW microwave treatments, the 2·0 kW microwave resulted in significant leakage of proteins and DNA from spores due to injury to the spore structure. TEM revealed that 2·0 kW microwave irradiation affected spore cortex hydrolysis and swelling, and ruptured the spore coat and inner membrane. Conclusions: These results suggest that 2·0 kW microwave irradiation ruptures the spore coat and inner membrane, and is significantly different from boiling. Significance and Impact of the Study: This study provides information on the sporicidal mechanisms of microwave irradiation on B. licheniformis spores.

UR - http://www.scopus.com/inward/record.url?scp=60149102307&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60149102307&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2672.2008.04056.x

DO - 10.1111/j.1365-2672.2008.04056.x

M3 - Article

C2 - 19191970

AN - SCOPUS:60149102307

VL - 106

SP - 877

EP - 885

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 3

ER -