Many prevention and treatment have been developed to resolve the increasing problem of the osteoporotic fractures. Some researchers proposed positive effects of whole body vibration (WBV) on osteoporotic trabecular bones of animals. However, there were few studies for detecting and tracking the effect of mechanical stimulation on osteoporotic bone. In the present study, the correlation between the improvement of morphological & mechanical characteristics and the effect of WBV were investigated and analyzed in OVX rats. The rats were randomly assigned to 5 groups: control, sham, WBV 17Hz, WBV 30Hz and WBV 45Hz. The WBV groups were exercised on a vibration platform (magnitude: 1mmp-p, frequency: 17Hz, 30Hz and 45Hz, 30minutes/day, 5days/week). The 4th lumbar vertebrae of rats were scanned by in-vivo micro computed tomography (micro-CT) at week 0 (just before WBV) and week 8 (after WBV). Structural parameters of the 4th lumbar vertebrae were investigated and analyzed. The quantitative decrement rates of trabecular bone on WBV groups with 30Hz and 45Hz were lower than those of control groups. For WBV group with 45Hz, the decrement rate of trabecular bone and its relative modulus were smaller than those of the control group. The results showed the beneficial effect of WBV on trabecular bones of OVX rats.