Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Vincent O. Baron; Mingzhou Chen; Simon O. Clark; Ann Williams; Kishan Dholakia; Stephen H. Gillespie

doi:10.1007/978-1-4939-7638-6_4

Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams, Kishan Dholakia, Stephen H. Gillespie

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

10 Citations (Scopus)

Abstract

Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	41-50
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7638-6_4
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1736
ISSN (Print)	1064-3745

Bibliographical note

Funding Information:
This work was supported by the PreDiCT-TB consortium [IMI Joint undertaking grant agreement number 115337, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007-2013) and EFPIA companies’ in kind contribution (www.imi.europa.eu). This work was supported by the Department of Health, UK. The views expressed in this chapter are those of the authors and not necessarily those of the Department of Health. This work was supported by the UK Engineering and Physical Sciences Research Council (Grant code EP/J01771X/1) and a European Union FAMOS project (FP7 ICT, 317744). Authors acknowledge the loan of a laser from M Squared Lasers.

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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10.1007/978-1-4939-7638-6_4

Cite this

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abstract = "Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.",

author = "Baron, {Vincent O.} and Mingzhou Chen and Clark, {Simon O.} and Ann Williams and Kishan Dholakia and Gillespie, {Stephen H.}",

note = "Funding Information: This work was supported by the PreDiCT-TB consortium [IMI Joint undertaking grant agreement number 115337, resources of which are composed of financial contribution from the European Union{\textquoteright}s Seventh Framework Programme (FP7/2007-2013) and EFPIA companies{\textquoteright} in kind contribution (www.imi.europa.eu). This work was supported by the Department of Health, UK. The views expressed in this chapter are those of the authors and not necessarily those of the Department of Health. This work was supported by the UK Engineering and Physical Sciences Research Council (Grant code EP/J01771X/1) and a European Union FAMOS project (FP7 ICT, 317744). Authors acknowledge the loan of a laser from M Squared Lasers. Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC.",

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AU - Clark, Simon O.

AU - Williams, Ann

AU - Dholakia, Kishan

AU - Gillespie, Stephen H.

N1 - Funding Information: This work was supported by the PreDiCT-TB consortium [IMI Joint undertaking grant agreement number 115337, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007-2013) and EFPIA companies’ in kind contribution (www.imi.europa.eu). This work was supported by the Department of Health, UK. The views expressed in this chapter are those of the authors and not necessarily those of the Department of Health. This work was supported by the UK Engineering and Physical Sciences Research Council (Grant code EP/J01771X/1) and a European Union FAMOS project (FP7 ICT, 317744). Authors acknowledge the loan of a laser from M Squared Lasers. Publisher Copyright: © 2018, Springer Science+Business Media, LLC.

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N2 - Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

AB - Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

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Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Abstract

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