Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy

Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams, Kishan Dholakia, Stephen H. Gillespie

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated. This methodology could be utilized to study the phenotype of mycobacterial cells in other tissues.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages41-50
Number of pages10
DOIs
Publication statusPublished - 2018 Jan 1

Publication series

NameMethods in Molecular Biology
Volume1736
ISSN (Print)1064-3745

Fingerprint

Raman Spectrum Analysis
Mycobacterium tuberculosis
Lipids
Frozen Sections
Anti-Bacterial Agents
Bacteria
Phenotype
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Baron, V. O., Chen, M., Clark, S. O., Williams, A., Dholakia, K., & Gillespie, S. H. (2018). Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy. In Methods in Molecular Biology (pp. 41-50). (Methods in Molecular Biology; Vol. 1736). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7638-6_4
Baron, Vincent O. ; Chen, Mingzhou ; Clark, Simon O. ; Williams, Ann ; Dholakia, Kishan ; Gillespie, Stephen H. / Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 41-50 (Methods in Molecular Biology).
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Baron, VO, Chen, M, Clark, SO, Williams, A, Dholakia, K & Gillespie, SH 2018, Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1736, Humana Press Inc., pp. 41-50. https://doi.org/10.1007/978-1-4939-7638-6_4

Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy. / Baron, Vincent O.; Chen, Mingzhou; Clark, Simon O.; Williams, Ann; Dholakia, Kishan; Gillespie, Stephen H.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 41-50 (Methods in Molecular Biology; Vol. 1736).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Baron VO, Chen M, Clark SO, Williams A, Dholakia K, Gillespie SH. Detecting phenotypically resistant Mycobacterium tuberculosis using wavelength modulated Raman spectroscopy. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 41-50. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7638-6_4