Detection of apoptosis using the C2A domain of synaptotagmin I

Hyo Il Jung, Mikko I. Kettunen, Bazbek Davletov, Kevin M. Brindle

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Binding of annexin V or the C2A domain of synaptotagmin I to phosphatidylserine expressed on the surface of apoptotic cells can, when labeled with appropriate probe molecules, be used to detect the presence of apoptosis using radionuclide, magnetic resonance, and optical imaging techniques. The preparation of a biotinylated C2A-GST fusion protein is described, and its capability, when used in conjunction with fluorescein-labeled streptavidin, of detecting apoptotic cells by flow cytometry is compared directly with the performance of a commercial preparation of fluorescein-labeled annexin V. Biotinylated C2A-GST, when used in conjunction with streptavidin-conjugated superparamagnetic iron oxide nanoparticles or Gd-chelate-avidin conjugates, was shown to be capable of detecting apoptotic cells using T2-weighted or T1-weighted magnetic resonance imaging experiments, respectively.

Original languageEnglish
Pages (from-to)983-987
Number of pages5
JournalBioconjugate Chemistry
Volume15
Issue number5
DOIs
Publication statusPublished - 2004 Sep 1

Fingerprint

Synaptotagmin I
Cell death
Streptavidin
Annexin A5
Cells
Magnetic resonance
Apoptosis
Fluorescein
Magnetic Resonance Imaging
Imaging techniques
Flow cytometry
Avidin
Optical Imaging
Phosphatidylserines
Iron oxides
Radioisotopes
Nanoparticles
Flow Cytometry
Fusion reactions
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Jung, Hyo Il ; Kettunen, Mikko I. ; Davletov, Bazbek ; Brindle, Kevin M. / Detection of apoptosis using the C2A domain of synaptotagmin I. In: Bioconjugate Chemistry. 2004 ; Vol. 15, No. 5. pp. 983-987.
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Detection of apoptosis using the C2A domain of synaptotagmin I. / Jung, Hyo Il; Kettunen, Mikko I.; Davletov, Bazbek; Brindle, Kevin M.

In: Bioconjugate Chemistry, Vol. 15, No. 5, 01.09.2004, p. 983-987.

Research output: Contribution to journalArticle

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T1 - Detection of apoptosis using the C2A domain of synaptotagmin I

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AB - Binding of annexin V or the C2A domain of synaptotagmin I to phosphatidylserine expressed on the surface of apoptotic cells can, when labeled with appropriate probe molecules, be used to detect the presence of apoptosis using radionuclide, magnetic resonance, and optical imaging techniques. The preparation of a biotinylated C2A-GST fusion protein is described, and its capability, when used in conjunction with fluorescein-labeled streptavidin, of detecting apoptotic cells by flow cytometry is compared directly with the performance of a commercial preparation of fluorescein-labeled annexin V. Biotinylated C2A-GST, when used in conjunction with streptavidin-conjugated superparamagnetic iron oxide nanoparticles or Gd-chelate-avidin conjugates, was shown to be capable of detecting apoptotic cells using T2-weighted or T1-weighted magnetic resonance imaging experiments, respectively.

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