Detection of biomarkers with carbon nanotube-based immunosensors.

Samuel Sánchez, Esteve Fàbregas, Martin Pumera

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A facile and capable method of preparation of sensitive carbon nanotube (CNT)/polysulfone/RIgG immunosensor is discussed in this chapter. The immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. CNT/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. This configuration offers large surface area, elevated porosity, and mechanical flexibility. The comparison with graphite/polysulfone/RIgG immunosensors shows a significantly improved sensitivity for those prepared with CNTs coupled with polysulfone (PSf). The immunosensing scheme is based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). The RIgG is incorporated into the PSf immunosensor using a phase inversion method. Horseradish peroxidase enzyme is used as label and hydroquinone as electrochemical mediator. The limit of detection for competitive assay is 1.66 microg/mL. The sensitivity is six times higher for MWCNT-based than for graphite-based electrodes.

Original languageEnglish
Pages (from-to)227-237
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume625
DOIs
Publication statusPublished - 2010

Fingerprint

Carbon Nanotubes
Biomarkers
Rabbits
Immunoglobulin G
Graphite
Electrodes
Porosity
Horseradish Peroxidase
Transducers
Limit of Detection
Binding Sites
polysulfone P 1700
Membranes
Enzymes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

@article{c3b862cad02547259b4920e0cc072824,
title = "Detection of biomarkers with carbon nanotube-based immunosensors.",
abstract = "A facile and capable method of preparation of sensitive carbon nanotube (CNT)/polysulfone/RIgG immunosensor is discussed in this chapter. The immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. CNT/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. This configuration offers large surface area, elevated porosity, and mechanical flexibility. The comparison with graphite/polysulfone/RIgG immunosensors shows a significantly improved sensitivity for those prepared with CNTs coupled with polysulfone (PSf). The immunosensing scheme is based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). The RIgG is incorporated into the PSf immunosensor using a phase inversion method. Horseradish peroxidase enzyme is used as label and hydroquinone as electrochemical mediator. The limit of detection for competitive assay is 1.66 microg/mL. The sensitivity is six times higher for MWCNT-based than for graphite-based electrodes.",
author = "Samuel S{\'a}nchez and Esteve F{\`a}bregas and Martin Pumera",
year = "2010",
doi = "10.1007/978-1-60761-579-8_19",
language = "English",
volume = "625",
pages = "227--237",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

Detection of biomarkers with carbon nanotube-based immunosensors. / Sánchez, Samuel; Fàbregas, Esteve; Pumera, Martin.

In: Methods in molecular biology (Clifton, N.J.), Vol. 625, 2010, p. 227-237.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of biomarkers with carbon nanotube-based immunosensors.

AU - Sánchez, Samuel

AU - Fàbregas, Esteve

AU - Pumera, Martin

PY - 2010

Y1 - 2010

N2 - A facile and capable method of preparation of sensitive carbon nanotube (CNT)/polysulfone/RIgG immunosensor is discussed in this chapter. The immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. CNT/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. This configuration offers large surface area, elevated porosity, and mechanical flexibility. The comparison with graphite/polysulfone/RIgG immunosensors shows a significantly improved sensitivity for those prepared with CNTs coupled with polysulfone (PSf). The immunosensing scheme is based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). The RIgG is incorporated into the PSf immunosensor using a phase inversion method. Horseradish peroxidase enzyme is used as label and hydroquinone as electrochemical mediator. The limit of detection for competitive assay is 1.66 microg/mL. The sensitivity is six times higher for MWCNT-based than for graphite-based electrodes.

AB - A facile and capable method of preparation of sensitive carbon nanotube (CNT)/polysulfone/RIgG immunosensor is discussed in this chapter. The immunosensor is based on the modification of disposable screen-printed electrodes by phase inversion method. CNT/polysulfone membrane acts as the reservoir of immunomolecules as well as a transducer. This configuration offers large surface area, elevated porosity, and mechanical flexibility. The comparison with graphite/polysulfone/RIgG immunosensors shows a significantly improved sensitivity for those prepared with CNTs coupled with polysulfone (PSf). The immunosensing scheme is based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). The RIgG is incorporated into the PSf immunosensor using a phase inversion method. Horseradish peroxidase enzyme is used as label and hydroquinone as electrochemical mediator. The limit of detection for competitive assay is 1.66 microg/mL. The sensitivity is six times higher for MWCNT-based than for graphite-based electrodes.

UR - http://www.scopus.com/inward/record.url?scp=77956216535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956216535&partnerID=8YFLogxK

U2 - 10.1007/978-1-60761-579-8_19

DO - 10.1007/978-1-60761-579-8_19

M3 - Article

C2 - 20422394

AN - SCOPUS:77956216535

VL - 625

SP - 227

EP - 237

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -