Detection of Clostridium difficile toxin A/B genes by multiplex real-time PCR for the diagnosis of C. difficile infection

Heejung Kim, Seokhoon Jeong, Myungsook Kim, Yangsoon Lee, Kyungwon Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Toxigenic Clostridium difficile culture is considered to be the standard diagnostic method for the detection of C. difficile infection (CDI). Culture methods are time-consuming and although enzyme immunoassay is rapid and easy to use, it has low sensitivity. In the present study, the AdvanSure CD real-time (RT)-PCR kit (LG Life Sciences) was evaluated for its ability to detect C. difficile toxin A (tcdA) and B (tcdB) genes, simultaneously. A total of 127 fresh diarrhoeal stool specimens, submitted to the clinical microbiology laboratory for C. difficile culture, were tested. C. difficile toxins and toxin genes were detected with a VIDAS C. difficile A&B (VIDAS-CDAB) enzyme-linked fluorescent immunoassay (ELFA) and the AdvanSure RT-PCR kit, respectively, according to the manufacturers' instructions. Their performance was compared with a standard toxigenic culture method as a reference. The sensitivity, specificity and positive and negative predictive values using the AdvanSure RT-PCR kit were 100%, 98.3%, 84.6% and 100%, respectively, while those of the VIDAS-CDAB system were 63.6%, 100%, 100% and 96.6%, respectively. Four tcdA +/tcdB + strains of C. difficile were detected with the AdvanSure RT-PCR kit, which offers comparable sensitivity and specificity to the reference method with a turnaround time of ~3 hours.

Original languageEnglish
Pages (from-to)274-277
Number of pages4
JournalJournal of Medical Microbiology
Volume61
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

Fingerprint

Clostridium Infections
Clostridium difficile
Multiplex Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Genes
Sensitivity and Specificity
Biological Science Disciplines
Microbiology
Immunoenzyme Techniques
Immunoassay
Clostridium difficile tcdA protein
Clostridium difficile toxB protein
Enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

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title = "Detection of Clostridium difficile toxin A/B genes by multiplex real-time PCR for the diagnosis of C. difficile infection",
abstract = "Toxigenic Clostridium difficile culture is considered to be the standard diagnostic method for the detection of C. difficile infection (CDI). Culture methods are time-consuming and although enzyme immunoassay is rapid and easy to use, it has low sensitivity. In the present study, the AdvanSure CD real-time (RT)-PCR kit (LG Life Sciences) was evaluated for its ability to detect C. difficile toxin A (tcdA) and B (tcdB) genes, simultaneously. A total of 127 fresh diarrhoeal stool specimens, submitted to the clinical microbiology laboratory for C. difficile culture, were tested. C. difficile toxins and toxin genes were detected with a VIDAS C. difficile A&B (VIDAS-CDAB) enzyme-linked fluorescent immunoassay (ELFA) and the AdvanSure RT-PCR kit, respectively, according to the manufacturers' instructions. Their performance was compared with a standard toxigenic culture method as a reference. The sensitivity, specificity and positive and negative predictive values using the AdvanSure RT-PCR kit were 100{\%}, 98.3{\%}, 84.6{\%} and 100{\%}, respectively, while those of the VIDAS-CDAB system were 63.6{\%}, 100{\%}, 100{\%} and 96.6{\%}, respectively. Four tcdA +/tcdB + strains of C. difficile were detected with the AdvanSure RT-PCR kit, which offers comparable sensitivity and specificity to the reference method with a turnaround time of ~3 hours.",
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Detection of Clostridium difficile toxin A/B genes by multiplex real-time PCR for the diagnosis of C. difficile infection. / Kim, Heejung; Jeong, Seokhoon; Kim, Myungsook; Lee, Yangsoon; Lee, Kyungwon.

In: Journal of Medical Microbiology, Vol. 61, No. 2, 01.02.2012, p. 274-277.

Research output: Contribution to journalArticle

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