Detection of germline mutations in patients with epithelial ovarian cancer using multi-gene panels: Beyond BRCA1/2

Kyung Jin Eoh, Ji Eun Kim, Hyung Seok Park, Seung Tae Lee, Ji Soo Park, Jung Woo Han, Jung Yun Lee, Sunghoon Kim, Sang Wun Kim, Jae Hoon Kim, Young Tae Kim, Eun Ji Nam

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Abstract

Purpose Next-generation sequencing (NGS) allows simultaneous sequencing of multiple cancer susceptibility genes and may represent a more efficient and less expensive approach than sequential testing. We assessed the frequency of germline mutations in individuals with epithelial ovarian cancer (EOC), using multi-gene panels and NGS. Materials and Methods Patients with EOC (n=117) with/without a family history of breast or ovarian cancer were recruited consecutively, from March 2016 to December 2016. Germline DNA was sequenced using 35-gene NGS panel, in order to identify mutations. Upon the detection of a genetic alteration using the panel, results were cross-validated using direct sequencing. Results Thirty-eight patients (32.5%) had 39 pathogenic or likely pathogenic mutations in eight genes, including BRCA1 (n=21), BRCA2 (n=10), BRIP1 (n=1), CHEK2 (n=2), MSH2 (n=1), POLE (n=1), RAD51C (n=2), and RAD51D (n=2). Among 64 patients with a family history of cancer, 27 (42.2%) had 27 pathogenic or likely pathogenic mutations, and six (9.3%) had mutations in genes other than BRCA1/2, such as CHECK2, MSH2, POLE, and RAD51C. Fifty-five patients (47.0%) were identified to carry only variants of uncertain significance. Conclusion Using the multi-gene panel test, we found that, of all patients included in our study, 32.5% had germline cancer-predisposing mutations. NGS was confirmed to substantially improve the detection rates of a wide spectrum of mutations in EOC patients compared with those obtained with the BRCA1/2 testing alone.

Original languageEnglish
Pages (from-to)917-925
Number of pages9
JournalCancer Research and Treatment
Volume50
Issue number3
DOIs
Publication statusPublished - 2018 Jul 1

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Eoh, K. J., Kim, J. E., Park, H. S., Lee, S. T., Park, J. S., Han, J. W., Lee, J. Y., Kim, S., Kim, S. W., Kim, J. H., Kim, Y. T., & Nam, E. J. (2018). Detection of germline mutations in patients with epithelial ovarian cancer using multi-gene panels: Beyond BRCA1/2. Cancer Research and Treatment, 50(3), 917-925. https://doi.org/10.4143/crt.2017.220