Detection of IFN-γ for latent tuberculosis diagnosis using an anodized aluminum oxide-based capacitive sensor

Joo Hyoung Kim, Young Wook Chang, Eun Bok, Hyun Jeong Kim, Hyejon Lee, Sang Nae Cho, Jeon Soo Shin, Kyung Hwa Yoo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We describe a rapid, sensitive, and label-free method to detect interferon-gamma (IFN-γ), a biomarker of latent tuberculosis infection (LTBI). IFN-γ is detected by measuring the capacitance change caused by its binding to an anti-IFN-γ antibody. The antibody is immobilized on the surface of an anodized aluminum oxide (AAO)-based capacitive sensor. With this technique, IFN-γ can be detected in the range of ~0.1. pg/ml to ~10. ng/ml, with a detection limit of 0.2. pg/ml. We have also measured the concentration of IFN-γ in clinical samples using the AAO-based capacitive sensor and compared this concentration with the results of the commercial QuantiFERON-TB Gold (QFT-G) ELISA kit to determine whether the two sets of data are consistent. Comparable results were obtained with the two measurement strategies, demonstrating the applicability of the AAO-based capacitive sensor to the diagnosis of LTBI.

Original languageEnglish
Pages (from-to)366-370
Number of pages5
JournalBiosensors and Bioelectronics
Volume51
DOIs
Publication statusPublished - 2014 Jan 15

Fingerprint

Interferons
Latent Tuberculosis
Capacitive sensors
Aluminum Oxide
Interferon-gamma
Aluminum
Oxides
Antibodies
Immobilized Antibodies
Biomarkers
Gold
Limit of Detection
Labels
Capacitance
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Kim, Joo Hyoung ; Chang, Young Wook ; Bok, Eun ; Kim, Hyun Jeong ; Lee, Hyejon ; Cho, Sang Nae ; Shin, Jeon Soo ; Yoo, Kyung Hwa. / Detection of IFN-γ for latent tuberculosis diagnosis using an anodized aluminum oxide-based capacitive sensor. In: Biosensors and Bioelectronics. 2014 ; Vol. 51. pp. 366-370.
@article{e91f472e3afc4da4b258963905eb2c73,
title = "Detection of IFN-γ for latent tuberculosis diagnosis using an anodized aluminum oxide-based capacitive sensor",
abstract = "We describe a rapid, sensitive, and label-free method to detect interferon-gamma (IFN-γ), a biomarker of latent tuberculosis infection (LTBI). IFN-γ is detected by measuring the capacitance change caused by its binding to an anti-IFN-γ antibody. The antibody is immobilized on the surface of an anodized aluminum oxide (AAO)-based capacitive sensor. With this technique, IFN-γ can be detected in the range of ~0.1. pg/ml to ~10. ng/ml, with a detection limit of 0.2. pg/ml. We have also measured the concentration of IFN-γ in clinical samples using the AAO-based capacitive sensor and compared this concentration with the results of the commercial QuantiFERON-TB Gold (QFT-G) ELISA kit to determine whether the two sets of data are consistent. Comparable results were obtained with the two measurement strategies, demonstrating the applicability of the AAO-based capacitive sensor to the diagnosis of LTBI.",
author = "Kim, {Joo Hyoung} and Chang, {Young Wook} and Eun Bok and Kim, {Hyun Jeong} and Hyejon Lee and Cho, {Sang Nae} and Shin, {Jeon Soo} and Yoo, {Kyung Hwa}",
year = "2014",
month = "1",
day = "15",
doi = "10.1016/j.bios.2013.08.013",
language = "English",
volume = "51",
pages = "366--370",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Limited",

}

Detection of IFN-γ for latent tuberculosis diagnosis using an anodized aluminum oxide-based capacitive sensor. / Kim, Joo Hyoung; Chang, Young Wook; Bok, Eun; Kim, Hyun Jeong; Lee, Hyejon; Cho, Sang Nae; Shin, Jeon Soo; Yoo, Kyung Hwa.

In: Biosensors and Bioelectronics, Vol. 51, 15.01.2014, p. 366-370.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of IFN-γ for latent tuberculosis diagnosis using an anodized aluminum oxide-based capacitive sensor

AU - Kim, Joo Hyoung

AU - Chang, Young Wook

AU - Bok, Eun

AU - Kim, Hyun Jeong

AU - Lee, Hyejon

AU - Cho, Sang Nae

AU - Shin, Jeon Soo

AU - Yoo, Kyung Hwa

PY - 2014/1/15

Y1 - 2014/1/15

N2 - We describe a rapid, sensitive, and label-free method to detect interferon-gamma (IFN-γ), a biomarker of latent tuberculosis infection (LTBI). IFN-γ is detected by measuring the capacitance change caused by its binding to an anti-IFN-γ antibody. The antibody is immobilized on the surface of an anodized aluminum oxide (AAO)-based capacitive sensor. With this technique, IFN-γ can be detected in the range of ~0.1. pg/ml to ~10. ng/ml, with a detection limit of 0.2. pg/ml. We have also measured the concentration of IFN-γ in clinical samples using the AAO-based capacitive sensor and compared this concentration with the results of the commercial QuantiFERON-TB Gold (QFT-G) ELISA kit to determine whether the two sets of data are consistent. Comparable results were obtained with the two measurement strategies, demonstrating the applicability of the AAO-based capacitive sensor to the diagnosis of LTBI.

AB - We describe a rapid, sensitive, and label-free method to detect interferon-gamma (IFN-γ), a biomarker of latent tuberculosis infection (LTBI). IFN-γ is detected by measuring the capacitance change caused by its binding to an anti-IFN-γ antibody. The antibody is immobilized on the surface of an anodized aluminum oxide (AAO)-based capacitive sensor. With this technique, IFN-γ can be detected in the range of ~0.1. pg/ml to ~10. ng/ml, with a detection limit of 0.2. pg/ml. We have also measured the concentration of IFN-γ in clinical samples using the AAO-based capacitive sensor and compared this concentration with the results of the commercial QuantiFERON-TB Gold (QFT-G) ELISA kit to determine whether the two sets of data are consistent. Comparable results were obtained with the two measurement strategies, demonstrating the applicability of the AAO-based capacitive sensor to the diagnosis of LTBI.

UR - http://www.scopus.com/inward/record.url?scp=84883265015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883265015&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2013.08.013

DO - 10.1016/j.bios.2013.08.013

M3 - Article

C2 - 24001512

AN - SCOPUS:84883265015

VL - 51

SP - 366

EP - 370

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

ER -