Detection of keratinolytic proteinase in skin tissues from guinea pigs infected with Microsporum canis by an immunoperoxidase technique

Kwang Hoon Lee, Hee Weon Park, Jung Bock Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An immunoperoxidase technique was performed to detect keratinolytic proteinase (KPase) in sections of the skin taken from guinea pigs infected with Microsporum canis and in cultured M. canis using polyclonal antisera to purified KPase. Of tissue samples from guinea pigs infected with M. canis, all sections of erythematous lesions showed positive staining mainly in the horny layer and the hair follicles. Positive depositions were seen only at the level of the outer and inner root sheaths of the hair follicles in sections of skin lesions showing scales and crusts. However, sections from areas of alopecia following desquamation of the crust showed no depositions of bright red reaction products. The patterns of deposition of KPase according to the clinical course of experimental dermatophytosis were consistent with the existence of organisms observed by PAS and methenamine silver stains. These results suggest that KPase may be produced during infection with M. canis after the development of erythema and before desquamation of the crust.

Original languageEnglish
Pages (from-to)447-453
Number of pages7
JournalJournal of Dermatological Science
Volume1
Issue number6
DOIs
Publication statusPublished - 1990 Nov

Fingerprint

Microsporum
Immunoenzyme Techniques
Skin
Guinea Pigs
Tissue
Hair Follicle
Methenamine
Tinea
Alopecia
Erythema
Reaction products
Immune Sera
Coloring Agents
Staining and Labeling
keratinase
Infection

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

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title = "Detection of keratinolytic proteinase in skin tissues from guinea pigs infected with Microsporum canis by an immunoperoxidase technique",
abstract = "An immunoperoxidase technique was performed to detect keratinolytic proteinase (KPase) in sections of the skin taken from guinea pigs infected with Microsporum canis and in cultured M. canis using polyclonal antisera to purified KPase. Of tissue samples from guinea pigs infected with M. canis, all sections of erythematous lesions showed positive staining mainly in the horny layer and the hair follicles. Positive depositions were seen only at the level of the outer and inner root sheaths of the hair follicles in sections of skin lesions showing scales and crusts. However, sections from areas of alopecia following desquamation of the crust showed no depositions of bright red reaction products. The patterns of deposition of KPase according to the clinical course of experimental dermatophytosis were consistent with the existence of organisms observed by PAS and methenamine silver stains. These results suggest that KPase may be produced during infection with M. canis after the development of erythema and before desquamation of the crust.",
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Detection of keratinolytic proteinase in skin tissues from guinea pigs infected with Microsporum canis by an immunoperoxidase technique. / Lee, Kwang Hoon; Park, Hee Weon; Lee, Jung Bock.

In: Journal of Dermatological Science, Vol. 1, No. 6, 11.1990, p. 447-453.

Research output: Contribution to journalArticle

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