Abstract
An immunoperoxidase technique was performed to detect keratinolytic proteinase (KPase) in sections of the skin taken from guinea pigs infected with Microsporum canis and in cultured M. canis using polyclonal antisera to purified KPase. Of tissue samples from guinea pigs infected with M. canis, all sections of erythematous lesions showed positive staining mainly in the horny layer and the hair follicles. Positive depositions were seen only at the level of the outer and inner root sheaths of the hair follicles in sections of skin lesions showing scales and crusts. However, sections from areas of alopecia following desquamation of the crust showed no depositions of bright red reaction products. The patterns of deposition of KPase according to the clinical course of experimental dermatophytosis were consistent with the existence of organisms observed by PAS and methenamine silver stains. These results suggest that KPase may be produced during infection with M. canis after the development of erythema and before desquamation of the crust.
| Original language | English |
|---|---|
| Pages (from-to) | 447-453 |
| Number of pages | 7 |
| Journal | Journal of Dermatological Science |
| Volume | 1 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1990 Nov |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Dermatology
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Detection of keratinolytic proteinase in skin tissues from guinea pigs infected with Microsporum canis by an immunoperoxidase technique. / Lee, Kwang Hoon; Park, Hee Weon; Lee, Jung Bock.
In: Journal of Dermatological Science, Vol. 1, No. 6, 11.1990, p. 447-453.Research output: Contribution to journal › Article
TY - JOUR
T1 - Detection of keratinolytic proteinase in skin tissues from guinea pigs infected with Microsporum canis by an immunoperoxidase technique
AU - Lee, Kwang Hoon
AU - Park, Hee Weon
AU - Lee, Jung Bock
PY - 1990/11
Y1 - 1990/11
N2 - An immunoperoxidase technique was performed to detect keratinolytic proteinase (KPase) in sections of the skin taken from guinea pigs infected with Microsporum canis and in cultured M. canis using polyclonal antisera to purified KPase. Of tissue samples from guinea pigs infected with M. canis, all sections of erythematous lesions showed positive staining mainly in the horny layer and the hair follicles. Positive depositions were seen only at the level of the outer and inner root sheaths of the hair follicles in sections of skin lesions showing scales and crusts. However, sections from areas of alopecia following desquamation of the crust showed no depositions of bright red reaction products. The patterns of deposition of KPase according to the clinical course of experimental dermatophytosis were consistent with the existence of organisms observed by PAS and methenamine silver stains. These results suggest that KPase may be produced during infection with M. canis after the development of erythema and before desquamation of the crust.
AB - An immunoperoxidase technique was performed to detect keratinolytic proteinase (KPase) in sections of the skin taken from guinea pigs infected with Microsporum canis and in cultured M. canis using polyclonal antisera to purified KPase. Of tissue samples from guinea pigs infected with M. canis, all sections of erythematous lesions showed positive staining mainly in the horny layer and the hair follicles. Positive depositions were seen only at the level of the outer and inner root sheaths of the hair follicles in sections of skin lesions showing scales and crusts. However, sections from areas of alopecia following desquamation of the crust showed no depositions of bright red reaction products. The patterns of deposition of KPase according to the clinical course of experimental dermatophytosis were consistent with the existence of organisms observed by PAS and methenamine silver stains. These results suggest that KPase may be produced during infection with M. canis after the development of erythema and before desquamation of the crust.
UR - http://www.scopus.com/inward/record.url?scp=0025223512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025223512&partnerID=8YFLogxK
U2 - 10.1016/0923-1811(90)90015-6
DO - 10.1016/0923-1811(90)90015-6
M3 - Article
C2 - 2288900
AN - SCOPUS:0025223512
VL - 1
SP - 447
EP - 453
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
SN - 0923-1811
IS - 6
ER -