Detection of major putative periodontopathogens in Korean advanced adult periodontitis patients using a nucleic acid-based approach

Bong Kyu Choi, Seong Hee Park, Yun Jung Yoo, Seongho Choi, Jung Kiu Chai, Kyoo Sung Cho, Chong Kwan Kim

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Abstract

Background: Although extensive microbial analyses have been performed from subgingival plaque samples of periodontitis patients, systematic analysis of subgingival microbiota has not been carried out in a Korean population so far. The purpose of this study was to describe the prevalence of major putative periodontopathogens in Korean patients by culture-independent methods. Methods: A total of 244 subgingival plaque samples (5 sites in each participant) were taken from 29 advanced adult periodontitis (AP) patients and 20 periodontally healthy subjects. AP samples were obtained from the 4 deepest periodontal pockets (≥6 mm probing depth [PD]) and 1 healthy site (≤3 mm PD) in each patient. Polymerase chain reaction (PCR) of 16S ribosomal DNA (rDNA) of subgingival plaque bacteria was performed with eubacterial primers. Aliquots of PCR products were then applied on nylon membranes and hybridized with specific oligonucleotide probes labeled with digoxigenin. Results: All diseased sites harbored Fusobacterium sp., while Porphyromonas gingivalis, Treponema sp., and Bacteroides forsythus were detected in more than 96% of 116 diseased sites. Peptostreptococcus micros, Actinobacillus actinomycetemcomitans, and Preuotella intermedia were present in 82%, 74%, and 71% of diseased sites, respectively. In sites of periodontally healthy subjects, Fusobacterium sp. was present in the highest proportion (58%). Treponema sp., P. gingivalis, and B. forsythus were detected in 22%, 18%, and 18% of healthy sites, respectively. P. micros, P. intermedia, and A. actinomycetemcomitans were found in 8%, 2%, and 1% of healthy sites, respectively. The prevalence of the periodontopathogens, with the exceptions of Fusobacterium sp. and B. forsythus, was significantly higher in the healthy sites of periodontitis subjects than in the healthy sites of periodontally healthy subjects (P<0.05). Conclusions: using highly sensitive methods relying on 16S ribosomal RNA-based oligonucleotide probes, we confirmed the strong association of 7 putative periodontopathogens with AP patients in a Korean population. With the exceptions of Fusobacterium sp. and B. forsythus, all the periodontopathogens were significantly more associated with the healthy sites of periodontitis subjects than in the healthy sites of periodontally healthy subjects.

Original languageEnglish
Pages (from-to)1387-1394
Number of pages8
JournalJournal of Periodontology
Volume71
Issue number9
DOIs
Publication statusPublished - 2000 Jan 1

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Chronic Periodontitis
Fusobacterium
Nucleic Acids
Healthy Volunteers
Periodontitis
Treponema
Aggregatibacter actinomycetemcomitans
Porphyromonas gingivalis
Oligonucleotide Probes
16S Ribosomal RNA
Peptostreptococcus
Periodontal Pocket
Digoxigenin
Polymerase Chain Reaction
Microbiota
Nylons
Ribosomal DNA
Population
Bacteria
Membranes

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Choi, Bong Kyu ; Park, Seong Hee ; Yoo, Yun Jung ; Choi, Seongho ; Chai, Jung Kiu ; Cho, Kyoo Sung ; Kim, Chong Kwan. / Detection of major putative periodontopathogens in Korean advanced adult periodontitis patients using a nucleic acid-based approach. In: Journal of Periodontology. 2000 ; Vol. 71, No. 9. pp. 1387-1394.
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title = "Detection of major putative periodontopathogens in Korean advanced adult periodontitis patients using a nucleic acid-based approach",
abstract = "Background: Although extensive microbial analyses have been performed from subgingival plaque samples of periodontitis patients, systematic analysis of subgingival microbiota has not been carried out in a Korean population so far. The purpose of this study was to describe the prevalence of major putative periodontopathogens in Korean patients by culture-independent methods. Methods: A total of 244 subgingival plaque samples (5 sites in each participant) were taken from 29 advanced adult periodontitis (AP) patients and 20 periodontally healthy subjects. AP samples were obtained from the 4 deepest periodontal pockets (≥6 mm probing depth [PD]) and 1 healthy site (≤3 mm PD) in each patient. Polymerase chain reaction (PCR) of 16S ribosomal DNA (rDNA) of subgingival plaque bacteria was performed with eubacterial primers. Aliquots of PCR products were then applied on nylon membranes and hybridized with specific oligonucleotide probes labeled with digoxigenin. Results: All diseased sites harbored Fusobacterium sp., while Porphyromonas gingivalis, Treponema sp., and Bacteroides forsythus were detected in more than 96{\%} of 116 diseased sites. Peptostreptococcus micros, Actinobacillus actinomycetemcomitans, and Preuotella intermedia were present in 82{\%}, 74{\%}, and 71{\%} of diseased sites, respectively. In sites of periodontally healthy subjects, Fusobacterium sp. was present in the highest proportion (58{\%}). Treponema sp., P. gingivalis, and B. forsythus were detected in 22{\%}, 18{\%}, and 18{\%} of healthy sites, respectively. P. micros, P. intermedia, and A. actinomycetemcomitans were found in 8{\%}, 2{\%}, and 1{\%} of healthy sites, respectively. The prevalence of the periodontopathogens, with the exceptions of Fusobacterium sp. and B. forsythus, was significantly higher in the healthy sites of periodontitis subjects than in the healthy sites of periodontally healthy subjects (P<0.05). Conclusions: using highly sensitive methods relying on 16S ribosomal RNA-based oligonucleotide probes, we confirmed the strong association of 7 putative periodontopathogens with AP patients in a Korean population. With the exceptions of Fusobacterium sp. and B. forsythus, all the periodontopathogens were significantly more associated with the healthy sites of periodontitis subjects than in the healthy sites of periodontally healthy subjects.",
author = "Choi, {Bong Kyu} and Park, {Seong Hee} and Yoo, {Yun Jung} and Seongho Choi and Chai, {Jung Kiu} and Cho, {Kyoo Sung} and Kim, {Chong Kwan}",
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Detection of major putative periodontopathogens in Korean advanced adult periodontitis patients using a nucleic acid-based approach. / Choi, Bong Kyu; Park, Seong Hee; Yoo, Yun Jung; Choi, Seongho; Chai, Jung Kiu; Cho, Kyoo Sung; Kim, Chong Kwan.

In: Journal of Periodontology, Vol. 71, No. 9, 01.01.2000, p. 1387-1394.

Research output: Contribution to journalArticle

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T1 - Detection of major putative periodontopathogens in Korean advanced adult periodontitis patients using a nucleic acid-based approach

AU - Choi, Bong Kyu

AU - Park, Seong Hee

AU - Yoo, Yun Jung

AU - Choi, Seongho

AU - Chai, Jung Kiu

AU - Cho, Kyoo Sung

AU - Kim, Chong Kwan

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Background: Although extensive microbial analyses have been performed from subgingival plaque samples of periodontitis patients, systematic analysis of subgingival microbiota has not been carried out in a Korean population so far. The purpose of this study was to describe the prevalence of major putative periodontopathogens in Korean patients by culture-independent methods. Methods: A total of 244 subgingival plaque samples (5 sites in each participant) were taken from 29 advanced adult periodontitis (AP) patients and 20 periodontally healthy subjects. AP samples were obtained from the 4 deepest periodontal pockets (≥6 mm probing depth [PD]) and 1 healthy site (≤3 mm PD) in each patient. Polymerase chain reaction (PCR) of 16S ribosomal DNA (rDNA) of subgingival plaque bacteria was performed with eubacterial primers. Aliquots of PCR products were then applied on nylon membranes and hybridized with specific oligonucleotide probes labeled with digoxigenin. Results: All diseased sites harbored Fusobacterium sp., while Porphyromonas gingivalis, Treponema sp., and Bacteroides forsythus were detected in more than 96% of 116 diseased sites. Peptostreptococcus micros, Actinobacillus actinomycetemcomitans, and Preuotella intermedia were present in 82%, 74%, and 71% of diseased sites, respectively. In sites of periodontally healthy subjects, Fusobacterium sp. was present in the highest proportion (58%). Treponema sp., P. gingivalis, and B. forsythus were detected in 22%, 18%, and 18% of healthy sites, respectively. P. micros, P. intermedia, and A. actinomycetemcomitans were found in 8%, 2%, and 1% of healthy sites, respectively. The prevalence of the periodontopathogens, with the exceptions of Fusobacterium sp. and B. forsythus, was significantly higher in the healthy sites of periodontitis subjects than in the healthy sites of periodontally healthy subjects (P<0.05). Conclusions: using highly sensitive methods relying on 16S ribosomal RNA-based oligonucleotide probes, we confirmed the strong association of 7 putative periodontopathogens with AP patients in a Korean population. With the exceptions of Fusobacterium sp. and B. forsythus, all the periodontopathogens were significantly more associated with the healthy sites of periodontitis subjects than in the healthy sites of periodontally healthy subjects.

AB - Background: Although extensive microbial analyses have been performed from subgingival plaque samples of periodontitis patients, systematic analysis of subgingival microbiota has not been carried out in a Korean population so far. The purpose of this study was to describe the prevalence of major putative periodontopathogens in Korean patients by culture-independent methods. Methods: A total of 244 subgingival plaque samples (5 sites in each participant) were taken from 29 advanced adult periodontitis (AP) patients and 20 periodontally healthy subjects. AP samples were obtained from the 4 deepest periodontal pockets (≥6 mm probing depth [PD]) and 1 healthy site (≤3 mm PD) in each patient. Polymerase chain reaction (PCR) of 16S ribosomal DNA (rDNA) of subgingival plaque bacteria was performed with eubacterial primers. Aliquots of PCR products were then applied on nylon membranes and hybridized with specific oligonucleotide probes labeled with digoxigenin. Results: All diseased sites harbored Fusobacterium sp., while Porphyromonas gingivalis, Treponema sp., and Bacteroides forsythus were detected in more than 96% of 116 diseased sites. Peptostreptococcus micros, Actinobacillus actinomycetemcomitans, and Preuotella intermedia were present in 82%, 74%, and 71% of diseased sites, respectively. In sites of periodontally healthy subjects, Fusobacterium sp. was present in the highest proportion (58%). Treponema sp., P. gingivalis, and B. forsythus were detected in 22%, 18%, and 18% of healthy sites, respectively. P. micros, P. intermedia, and A. actinomycetemcomitans were found in 8%, 2%, and 1% of healthy sites, respectively. The prevalence of the periodontopathogens, with the exceptions of Fusobacterium sp. and B. forsythus, was significantly higher in the healthy sites of periodontitis subjects than in the healthy sites of periodontally healthy subjects (P<0.05). Conclusions: using highly sensitive methods relying on 16S ribosomal RNA-based oligonucleotide probes, we confirmed the strong association of 7 putative periodontopathogens with AP patients in a Korean population. With the exceptions of Fusobacterium sp. and B. forsythus, all the periodontopathogens were significantly more associated with the healthy sites of periodontitis subjects than in the healthy sites of periodontally healthy subjects.

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