Detection of Mycobacterium tuberculosis in clinical samples from patients with tuberculosis or other pulmonary diseases by polymerase chain reaction.

K. H. Yoon, Sangnae Cho, T. Y. Lee, S. H. Cheon, J. Chang, S. K. Kim, Y. Chong, D. H. Chung, W. Y. Lee, J. D. Kim

Research output: Contribution to journalArticle

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Abstract

Polymerase chain reaction (PCR) using primers targeting the IS6110 repetitive sequence was employed to detect Mycobacterium tuberculosis in 228 samples from patients with tuberculosis or other pulmonary diseases and controls, and the results were compared with culture and clinical findings. None of culture negative samples from 17 healthy controls were PCR positive. Of 109 active tuberculosis patients under chemotherapy, 88 (80.7%) were PCR positive and were significantly higher than 63 (57.8%) positive by culture. Fifty-nine (93.7) of 63 culture positive and 29 (63.0%) of 46 culture negative specimens contained M. tuberculosis detectable by PCR. In 41 specimens from inactive tuberculosis patients who visited to the chest clinic because of chest problems, 16 (39.0%) also gave PCR positive results. In addition, 14 (46.7%) of 30 specimens submitted for M. tuberculosis culture from patients with pulmonary diseases were PCR positive. Presumptive diagnosis of these PCR positive patients was bronchitis, pneumonia, bronchial asthma, etc. Therefore, this study suggests that PCR is sensitive and specific in detecting M. tuberculosis in clinical specimens. However, the interpretation of the PCR results in specimens from patients with pulmonary diseases should be done cautiously in areas with a high prevalence of tuberculosis.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalYonsei medical journal
Volume33
Issue number3
DOIs
Publication statusPublished - 1992 Jan 1

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Mycobacterium tuberculosis
Lung Diseases
Tuberculosis
Polymerase Chain Reaction
Thorax
Bronchitis
Nucleic Acid Repetitive Sequences
Pneumonia
Asthma
Drug Therapy

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Yoon, K. H. ; Cho, Sangnae ; Lee, T. Y. ; Cheon, S. H. ; Chang, J. ; Kim, S. K. ; Chong, Y. ; Chung, D. H. ; Lee, W. Y. ; Kim, J. D. / Detection of Mycobacterium tuberculosis in clinical samples from patients with tuberculosis or other pulmonary diseases by polymerase chain reaction. In: Yonsei medical journal. 1992 ; Vol. 33, No. 3. pp. 209-216.
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abstract = "Polymerase chain reaction (PCR) using primers targeting the IS6110 repetitive sequence was employed to detect Mycobacterium tuberculosis in 228 samples from patients with tuberculosis or other pulmonary diseases and controls, and the results were compared with culture and clinical findings. None of culture negative samples from 17 healthy controls were PCR positive. Of 109 active tuberculosis patients under chemotherapy, 88 (80.7{\%}) were PCR positive and were significantly higher than 63 (57.8{\%}) positive by culture. Fifty-nine (93.7) of 63 culture positive and 29 (63.0{\%}) of 46 culture negative specimens contained M. tuberculosis detectable by PCR. In 41 specimens from inactive tuberculosis patients who visited to the chest clinic because of chest problems, 16 (39.0{\%}) also gave PCR positive results. In addition, 14 (46.7{\%}) of 30 specimens submitted for M. tuberculosis culture from patients with pulmonary diseases were PCR positive. Presumptive diagnosis of these PCR positive patients was bronchitis, pneumonia, bronchial asthma, etc. Therefore, this study suggests that PCR is sensitive and specific in detecting M. tuberculosis in clinical specimens. However, the interpretation of the PCR results in specimens from patients with pulmonary diseases should be done cautiously in areas with a high prevalence of tuberculosis.",
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Detection of Mycobacterium tuberculosis in clinical samples from patients with tuberculosis or other pulmonary diseases by polymerase chain reaction. / Yoon, K. H.; Cho, Sangnae; Lee, T. Y.; Cheon, S. H.; Chang, J.; Kim, S. K.; Chong, Y.; Chung, D. H.; Lee, W. Y.; Kim, J. D.

In: Yonsei medical journal, Vol. 33, No. 3, 01.01.1992, p. 209-216.

Research output: Contribution to journalArticle

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AU - Cho, Sangnae

AU - Lee, T. Y.

AU - Cheon, S. H.

AU - Chang, J.

AU - Kim, S. K.

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AU - Lee, W. Y.

AU - Kim, J. D.

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N2 - Polymerase chain reaction (PCR) using primers targeting the IS6110 repetitive sequence was employed to detect Mycobacterium tuberculosis in 228 samples from patients with tuberculosis or other pulmonary diseases and controls, and the results were compared with culture and clinical findings. None of culture negative samples from 17 healthy controls were PCR positive. Of 109 active tuberculosis patients under chemotherapy, 88 (80.7%) were PCR positive and were significantly higher than 63 (57.8%) positive by culture. Fifty-nine (93.7) of 63 culture positive and 29 (63.0%) of 46 culture negative specimens contained M. tuberculosis detectable by PCR. In 41 specimens from inactive tuberculosis patients who visited to the chest clinic because of chest problems, 16 (39.0%) also gave PCR positive results. In addition, 14 (46.7%) of 30 specimens submitted for M. tuberculosis culture from patients with pulmonary diseases were PCR positive. Presumptive diagnosis of these PCR positive patients was bronchitis, pneumonia, bronchial asthma, etc. Therefore, this study suggests that PCR is sensitive and specific in detecting M. tuberculosis in clinical specimens. However, the interpretation of the PCR results in specimens from patients with pulmonary diseases should be done cautiously in areas with a high prevalence of tuberculosis.

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