Detection of specific serum IgE in clonorchiasis cases and analysis of Clonorchis sinensis allergens.

T. S. Yong, D. S. Kim, S. Y. Lee, K. I. Im, K. Y. Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Specific serum IgE levels of Clonorchis sinensis in infected humans were measured by avidin-biotin ELISA, and allergens from C. sinensis were identified by immunoblot and autoradiography. Then, allergens fractionated by Sephadex G-200 gel filtration were analyzed, and cross-reactive allergenic components of C. sinensis reacted with paragonimiasis sera were revealed. Fourteen out of 15 C. sinensis egg-positives were found to be serum IgE positive (absorbance > 0.27). Of 14 IgE-reacting allergen bands visualized, major allergens of 66, 61.5, 45, 37, 28.5, 23.5 and 15.5 KD were recognized by more than 50% of the sera of infected humans. The considerable individual variations of IgE immune responses to C. sinensis allergenic components were also noticed. C. sinensis extract was separated into 5 fractions by Sephadex G-200 gel filtration. Seventy-four KD allergen was recognized in the first fraction, 50, 45, 37, 29.5 and 28.5 KD in the third, and 15.5 KD in the fourth. Cross-reactive allergens with sera of paragonimiasis cases were identified as 66, 45, 28.5, 13 and 7.5 KD.

Original languageEnglish
Pages (from-to)248-257
Number of pages10
JournalYonsei medical journal
Volume34
Issue number3
DOIs
Publication statusPublished - 1993 Sep

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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