Detection of specific serum IgE in clonorchiasis cases and analysis of Clonorchis sinensis allergens.

Taisoon Yong, D. S. Kim, S. Y. Lee, K. I. Im, K. Y. Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Specific serum IgE levels of Clonorchis sinensis in infected humans were measured by avidin-biotin ELISA, and allergens from C. sinensis were identified by immunoblot and autoradiography. Then, allergens fractionated by Sephadex G-200 gel filtration were analyzed, and cross-reactive allergenic components of C. sinensis reacted with paragonimiasis sera were revealed. Fourteen out of 15 C. sinensis egg-positives were found to be serum IgE positive (absorbance > 0.27). Of 14 IgE-reacting allergen bands visualized, major allergens of 66, 61.5, 45, 37, 28.5, 23.5 and 15.5 KD were recognized by more than 50% of the sera of infected humans. The considerable individual variations of IgE immune responses to C. sinensis allergenic components were also noticed. C. sinensis extract was separated into 5 fractions by Sephadex G-200 gel filtration. Seventy-four KD allergen was recognized in the first fraction, 50, 45, 37, 29.5 and 28.5 KD in the third, and 15.5 KD in the fourth. Cross-reactive allergens with sera of paragonimiasis cases were identified as 66, 45, 28.5, 13 and 7.5 KD.

Original languageEnglish
Pages (from-to)248-257
Number of pages10
JournalYonsei medical journal
Volume34
Issue number3
DOIs
Publication statusPublished - 1993 Jan 1

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Clonorchiasis
Clonorchis sinensis
Allergens
Immunoglobulin E
Serum
Paragonimiasis
Gel Chromatography
Avidin
Biotin
Autoradiography
Ovum
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Yong, Taisoon ; Kim, D. S. ; Lee, S. Y. ; Im, K. I. ; Lee, K. Y. / Detection of specific serum IgE in clonorchiasis cases and analysis of Clonorchis sinensis allergens. In: Yonsei medical journal. 1993 ; Vol. 34, No. 3. pp. 248-257.
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Detection of specific serum IgE in clonorchiasis cases and analysis of Clonorchis sinensis allergens. / Yong, Taisoon; Kim, D. S.; Lee, S. Y.; Im, K. I.; Lee, K. Y.

In: Yonsei medical journal, Vol. 34, No. 3, 01.01.1993, p. 248-257.

Research output: Contribution to journalArticle

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