Detection of the most common corneal dystrophies caused by BIGH3 gene point mutations using a multispot gold-capped nanoparticle array chip

So Young Yoo, Do Kyun Kim, Tae Jung Park, Eungkweon Kim, Eiichi Tamiya, Sang Yup Lee

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The localized surface plasmon resonance (LSPR) optical property has recently been well employed as an effective platform for the quantitative detection of protein-protein interactions on the nanoscale. However, its advantage has not been fully explored yet in the DNA diagnosis field, especially in detecting point mutations of DNA. Point mutations of the BIGH3gene are associated with the most common corneal dystrophies (CDs), such as Avellino corneal dystrophy, Reis-Bucklers corneal dystrophy, and lattice corneal dystrophy. Since the detection of these corneal dystrophies is urgently needed before laserassisted in situ keratomileusis operation to prevent blindness, genetic analysis of the BIGH3gene is critical in most ophthalmological clinics. In this study, we report LSPRbased detection of the BIGH3gene mutations by using a multispot gold-capped nanoparticle array (MG-NPA) chip. The analytical range and sensitivity of the MG-NPA chip were determined by measuring different concentrations of each CD target DNA in the range of 1 fMto 1 μ M. Under the optimal conditions, the detection of DNA hybridization with each CD target DNA was performed with a detection limit of 1 pM target DNA. The selective discrimination against a single-base mismatch DNA sequence was also achieved by using both homozygous and heterozygous CD samples. It demonstrates that the label-free LSPR-based optical biosensor system employing the MG-NPA chip provides a new diagnostic platform allowing the selective and sensitive detection of various DNA point mutations, leading to possible diagnosis of mutation-related diseases including corneal dystrophies reported here.

Original languageEnglish
Pages (from-to)1349-1357
Number of pages9
JournalAnalytical Chemistry
Volume82
Issue number4
DOIs
Publication statusPublished - 2010 Feb 15

Fingerprint

Gold
Genes
Nanoparticles
DNA
Surface plasmon resonance
DNA sequences
Biosensors
Labels
Proteins
Optical properties

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Yoo, So Young ; Kim, Do Kyun ; Park, Tae Jung ; Kim, Eungkweon ; Tamiya, Eiichi ; Lee, Sang Yup. / Detection of the most common corneal dystrophies caused by BIGH3 gene point mutations using a multispot gold-capped nanoparticle array chip. In: Analytical Chemistry. 2010 ; Vol. 82, No. 4. pp. 1349-1357.
@article{11f30e7356ed4576be57b7c658bb2d6e,
title = "Detection of the most common corneal dystrophies caused by BIGH3 gene point mutations using a multispot gold-capped nanoparticle array chip",
abstract = "The localized surface plasmon resonance (LSPR) optical property has recently been well employed as an effective platform for the quantitative detection of protein-protein interactions on the nanoscale. However, its advantage has not been fully explored yet in the DNA diagnosis field, especially in detecting point mutations of DNA. Point mutations of the BIGH3gene are associated with the most common corneal dystrophies (CDs), such as Avellino corneal dystrophy, Reis-Bucklers corneal dystrophy, and lattice corneal dystrophy. Since the detection of these corneal dystrophies is urgently needed before laserassisted in situ keratomileusis operation to prevent blindness, genetic analysis of the BIGH3gene is critical in most ophthalmological clinics. In this study, we report LSPRbased detection of the BIGH3gene mutations by using a multispot gold-capped nanoparticle array (MG-NPA) chip. The analytical range and sensitivity of the MG-NPA chip were determined by measuring different concentrations of each CD target DNA in the range of 1 fMto 1 μ M. Under the optimal conditions, the detection of DNA hybridization with each CD target DNA was performed with a detection limit of 1 pM target DNA. The selective discrimination against a single-base mismatch DNA sequence was also achieved by using both homozygous and heterozygous CD samples. It demonstrates that the label-free LSPR-based optical biosensor system employing the MG-NPA chip provides a new diagnostic platform allowing the selective and sensitive detection of various DNA point mutations, leading to possible diagnosis of mutation-related diseases including corneal dystrophies reported here.",
author = "Yoo, {So Young} and Kim, {Do Kyun} and Park, {Tae Jung} and Eungkweon Kim and Eiichi Tamiya and Lee, {Sang Yup}",
year = "2010",
month = "2",
day = "15",
doi = "10.1021/ac902410z",
language = "English",
volume = "82",
pages = "1349--1357",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "4",

}

Detection of the most common corneal dystrophies caused by BIGH3 gene point mutations using a multispot gold-capped nanoparticle array chip. / Yoo, So Young; Kim, Do Kyun; Park, Tae Jung; Kim, Eungkweon; Tamiya, Eiichi; Lee, Sang Yup.

In: Analytical Chemistry, Vol. 82, No. 4, 15.02.2010, p. 1349-1357.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of the most common corneal dystrophies caused by BIGH3 gene point mutations using a multispot gold-capped nanoparticle array chip

AU - Yoo, So Young

AU - Kim, Do Kyun

AU - Park, Tae Jung

AU - Kim, Eungkweon

AU - Tamiya, Eiichi

AU - Lee, Sang Yup

PY - 2010/2/15

Y1 - 2010/2/15

N2 - The localized surface plasmon resonance (LSPR) optical property has recently been well employed as an effective platform for the quantitative detection of protein-protein interactions on the nanoscale. However, its advantage has not been fully explored yet in the DNA diagnosis field, especially in detecting point mutations of DNA. Point mutations of the BIGH3gene are associated with the most common corneal dystrophies (CDs), such as Avellino corneal dystrophy, Reis-Bucklers corneal dystrophy, and lattice corneal dystrophy. Since the detection of these corneal dystrophies is urgently needed before laserassisted in situ keratomileusis operation to prevent blindness, genetic analysis of the BIGH3gene is critical in most ophthalmological clinics. In this study, we report LSPRbased detection of the BIGH3gene mutations by using a multispot gold-capped nanoparticle array (MG-NPA) chip. The analytical range and sensitivity of the MG-NPA chip were determined by measuring different concentrations of each CD target DNA in the range of 1 fMto 1 μ M. Under the optimal conditions, the detection of DNA hybridization with each CD target DNA was performed with a detection limit of 1 pM target DNA. The selective discrimination against a single-base mismatch DNA sequence was also achieved by using both homozygous and heterozygous CD samples. It demonstrates that the label-free LSPR-based optical biosensor system employing the MG-NPA chip provides a new diagnostic platform allowing the selective and sensitive detection of various DNA point mutations, leading to possible diagnosis of mutation-related diseases including corneal dystrophies reported here.

AB - The localized surface plasmon resonance (LSPR) optical property has recently been well employed as an effective platform for the quantitative detection of protein-protein interactions on the nanoscale. However, its advantage has not been fully explored yet in the DNA diagnosis field, especially in detecting point mutations of DNA. Point mutations of the BIGH3gene are associated with the most common corneal dystrophies (CDs), such as Avellino corneal dystrophy, Reis-Bucklers corneal dystrophy, and lattice corneal dystrophy. Since the detection of these corneal dystrophies is urgently needed before laserassisted in situ keratomileusis operation to prevent blindness, genetic analysis of the BIGH3gene is critical in most ophthalmological clinics. In this study, we report LSPRbased detection of the BIGH3gene mutations by using a multispot gold-capped nanoparticle array (MG-NPA) chip. The analytical range and sensitivity of the MG-NPA chip were determined by measuring different concentrations of each CD target DNA in the range of 1 fMto 1 μ M. Under the optimal conditions, the detection of DNA hybridization with each CD target DNA was performed with a detection limit of 1 pM target DNA. The selective discrimination against a single-base mismatch DNA sequence was also achieved by using both homozygous and heterozygous CD samples. It demonstrates that the label-free LSPR-based optical biosensor system employing the MG-NPA chip provides a new diagnostic platform allowing the selective and sensitive detection of various DNA point mutations, leading to possible diagnosis of mutation-related diseases including corneal dystrophies reported here.

UR - http://www.scopus.com/inward/record.url?scp=76849096847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76849096847&partnerID=8YFLogxK

U2 - 10.1021/ac902410z

DO - 10.1021/ac902410z

M3 - Article

C2 - 20092310

AN - SCOPUS:76849096847

VL - 82

SP - 1349

EP - 1357

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 4

ER -