Detection of Treponema pallidum in tissue

a comparative study of the avidin-biotin-peroxidase complex, indirect immunoperoxidase, FTA-ABS complement techniques and the darkfield method.

W. S. Lee, Mingeol Lee, K. Y. Chung, J. B. Lee

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

With 37 formalin-fixed, paraffin embedded specimens from the lesions of 30 patients with primary, secondary or gastric syphilis, we performed avidin-biotin-peroxidase complex (ABC), indirect immunoperoxidase (IIP) and FTA-ABS complement techniques. Darkfield examination was done in 17 skin lesions. The immunoperoxidase technique, especially the ABC technique, revealed higher reactivity than the FTA-ABS complement technique and darkfield examination in detecting Treponema pallidum in tissues. Furthermore, the ABC technique produced less intense nonspecific background staining than the IIP technique. Histologically, most of the treponemes were located in the upper dermis, epidermis and vessel walls in the order named, and rarely in the lower dermis of the syphilitic skin lesions.

Original languageEnglish
Pages (from-to)335-341
Number of pages7
JournalYonsei Medical Journal
Volume32
Issue number4
DOIs
Publication statusPublished - 1991 Jan 1

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Treponema pallidum
Avidin
Biotin
Peroxidase
Dermis
Immunoenzyme Techniques
Skin
Syphilis
Epidermis
Paraffin
Formaldehyde
Stomach
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "With 37 formalin-fixed, paraffin embedded specimens from the lesions of 30 patients with primary, secondary or gastric syphilis, we performed avidin-biotin-peroxidase complex (ABC), indirect immunoperoxidase (IIP) and FTA-ABS complement techniques. Darkfield examination was done in 17 skin lesions. The immunoperoxidase technique, especially the ABC technique, revealed higher reactivity than the FTA-ABS complement technique and darkfield examination in detecting Treponema pallidum in tissues. Furthermore, the ABC technique produced less intense nonspecific background staining than the IIP technique. Histologically, most of the treponemes were located in the upper dermis, epidermis and vessel walls in the order named, and rarely in the lower dermis of the syphilitic skin lesions.",
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AU - Lee, J. B.

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