Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS

Mi Kyeong Lee; Seung Hyun Kim; Jin Ho Park; Jung Hee Cho; Do Hoon Kim; Ju Hyun Baek; Chang Hee Cho; Hyo Jin Kim; Mi Jeong Ahn; Ki Yong Lee; Sang Hyun Sung

doi:10.1080/10826070903127011

Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS

Mi Kyeong Lee, Seung Hyun Kim, Jin Ho Park, Jung Hee Cho, Do Hoon Kim, Ju Hyun Baek, Chang Hee Cho, Hyo Jin Kim, Mi Jeong Ahn, Ki Yong Lee, Sang Hyun Sung

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Simultaneous determination of four major constituents, paeoniflorin, baicalin, trans-cinnamic acid, and glycyrrhizin in Siho-Gyeoji-Tang (SGT) was established by an HPLC-DAD method for the quality control of traditional herbal medicinal preparation. Validation of the HPLC-DAD method showed good linearity (r²>0.997) of the four compounds analyzed in relatively wide concentration ranges. The R.S.D. values for intra-day and inter-day precision were less than 9.0% and the limits of detection (LOD) were less than 22ng. The mean recovery of each compound was 90.2-116.9%. In addition, analysis of ginsenosides R_b1, R_g1, saikosaponins a, b₂, and c were established by HPLC-MS in selected ion monitoring (SIM) mode, due to their low sensitivity in HPLC-DAD. These results suggest that the developed analytical method is simple and effective, and could be readily utilized as a quality control method for commercial SGT products.

Original language	English
Pages (from-to)	2122-2133
Number of pages	12
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	32
Issue number	14
DOIs	https://doi.org/10.1080/10826070903127011
Publication status	Published - 2009

Bibliographical note

Funding Information:
This research was supported by a grant of the Korea Food & Drug Administration, Republic of Korea (2005).

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

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10.1080/10826070903127011

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title = "Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS",

abstract = "Simultaneous determination of four major constituents, paeoniflorin, baicalin, trans-cinnamic acid, and glycyrrhizin in Siho-Gyeoji-Tang (SGT) was established by an HPLC-DAD method for the quality control of traditional herbal medicinal preparation. Validation of the HPLC-DAD method showed good linearity (r2>0.997) of the four compounds analyzed in relatively wide concentration ranges. The R.S.D. values for intra-day and inter-day precision were less than 9.0% and the limits of detection (LOD) were less than 22ng. The mean recovery of each compound was 90.2-116.9%. In addition, analysis of ginsenosides Rb1, Rg1, saikosaponins a, b2, and c were established by HPLC-MS in selected ion monitoring (SIM) mode, due to their low sensitivity in HPLC-DAD. These results suggest that the developed analytical method is simple and effective, and could be readily utilized as a quality control method for commercial SGT products.",

author = "Lee, {Mi Kyeong} and Kim, {Seung Hyun} and Park, {Jin Ho} and Cho, {Jung Hee} and Kim, {Do Hoon} and Baek, {Ju Hyun} and Cho, {Chang Hee} and Kim, {Hyo Jin} and Ahn, {Mi Jeong} and Lee, {Ki Yong} and Sung, {Sang Hyun}",

note = "Funding Information: This research was supported by a grant of the Korea Food & Drug Administration, Republic of Korea (2005).",

year = "2009",

doi = "10.1080/10826070903127011",

language = "English",

volume = "32",

pages = "2122--2133",

journal = "Journal of Liquid Chromatography and Related Technologies",

issn = "1082-6076",

publisher = "Taylor and Francis Ltd.",

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T1 - Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS

AU - Lee, Mi Kyeong

AU - Kim, Seung Hyun

AU - Park, Jin Ho

AU - Cho, Jung Hee

AU - Kim, Do Hoon

AU - Baek, Ju Hyun

AU - Cho, Chang Hee

AU - Kim, Hyo Jin

AU - Ahn, Mi Jeong

AU - Lee, Ki Yong

AU - Sung, Sang Hyun

N1 - Funding Information: This research was supported by a grant of the Korea Food & Drug Administration, Republic of Korea (2005).

PY - 2009

Y1 - 2009

N2 - Simultaneous determination of four major constituents, paeoniflorin, baicalin, trans-cinnamic acid, and glycyrrhizin in Siho-Gyeoji-Tang (SGT) was established by an HPLC-DAD method for the quality control of traditional herbal medicinal preparation. Validation of the HPLC-DAD method showed good linearity (r2>0.997) of the four compounds analyzed in relatively wide concentration ranges. The R.S.D. values for intra-day and inter-day precision were less than 9.0% and the limits of detection (LOD) were less than 22ng. The mean recovery of each compound was 90.2-116.9%. In addition, analysis of ginsenosides Rb1, Rg1, saikosaponins a, b2, and c were established by HPLC-MS in selected ion monitoring (SIM) mode, due to their low sensitivity in HPLC-DAD. These results suggest that the developed analytical method is simple and effective, and could be readily utilized as a quality control method for commercial SGT products.

AB - Simultaneous determination of four major constituents, paeoniflorin, baicalin, trans-cinnamic acid, and glycyrrhizin in Siho-Gyeoji-Tang (SGT) was established by an HPLC-DAD method for the quality control of traditional herbal medicinal preparation. Validation of the HPLC-DAD method showed good linearity (r2>0.997) of the four compounds analyzed in relatively wide concentration ranges. The R.S.D. values for intra-day and inter-day precision were less than 9.0% and the limits of detection (LOD) were less than 22ng. The mean recovery of each compound was 90.2-116.9%. In addition, analysis of ginsenosides Rb1, Rg1, saikosaponins a, b2, and c were established by HPLC-MS in selected ion monitoring (SIM) mode, due to their low sensitivity in HPLC-DAD. These results suggest that the developed analytical method is simple and effective, and could be readily utilized as a quality control method for commercial SGT products.

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Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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