Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS

Mi Kyeong Lee, Seung Hyun Kim, Jin Ho Park, Jung Hee Cho, Do Hoon Kim, Ju Hyun Baek, Chang Hee Cho, Hyo Jin Kim, Mi Jeong Ahn, Ki Yong Lee, Sang Hyun Sung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Simultaneous determination of four major constituents, paeoniflorin, baicalin, trans-cinnamic acid, and glycyrrhizin in Siho-Gyeoji-Tang (SGT) was established by an HPLC-DAD method for the quality control of traditional herbal medicinal preparation. Validation of the HPLC-DAD method showed good linearity (r2>0.997) of the four compounds analyzed in relatively wide concentration ranges. The R.S.D. values for intra-day and inter-day precision were less than 9.0% and the limits of detection (LOD) were less than 22ng. The mean recovery of each compound was 90.2-116.9%. In addition, analysis of ginsenosides Rb1, Rg1, saikosaponins a, b2, and c were established by HPLC-MS in selected ion monitoring (SIM) mode, due to their low sensitivity in HPLC-DAD. These results suggest that the developed analytical method is simple and effective, and could be readily utilized as a quality control method for commercial SGT products.

Original languageEnglish
Pages (from-to)2122-2133
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Volume32
Issue number14
DOIs
Publication statusPublished - 2009 Sep 25

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Quality control
High Pressure Liquid Chromatography
Glycyrrhizic Acid
Quality Control
Ions
Plant Preparations
Recovery
Monitoring
Limit of Detection
cinnamic acid
ginsenoside Rb1
baicalin
peoniflorin
saikosaponin

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

Cite this

Lee, Mi Kyeong ; Kim, Seung Hyun ; Park, Jin Ho ; Cho, Jung Hee ; Kim, Do Hoon ; Baek, Ju Hyun ; Cho, Chang Hee ; Kim, Hyo Jin ; Ahn, Mi Jeong ; Lee, Ki Yong ; Sung, Sang Hyun. / Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS. In: Journal of Liquid Chromatography and Related Technologies. 2009 ; Vol. 32, No. 14. pp. 2122-2133.
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Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS. / Lee, Mi Kyeong; Kim, Seung Hyun; Park, Jin Ho; Cho, Jung Hee; Kim, Do Hoon; Baek, Ju Hyun; Cho, Chang Hee; Kim, Hyo Jin; Ahn, Mi Jeong; Lee, Ki Yong; Sung, Sang Hyun.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 32, No. 14, 25.09.2009, p. 2122-2133.

Research output: Contribution to journalArticle

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AU - Lee, Mi Kyeong

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AU - Cho, Jung Hee

AU - Kim, Do Hoon

AU - Baek, Ju Hyun

AU - Cho, Chang Hee

AU - Kim, Hyo Jin

AU - Ahn, Mi Jeong

AU - Lee, Ki Yong

AU - Sung, Sang Hyun

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