Abstract
Simultaneous determination of four major constituents, paeoniflorin, baicalin, trans-cinnamic acid, and glycyrrhizin in Siho-Gyeoji-Tang (SGT) was established by an HPLC-DAD method for the quality control of traditional herbal medicinal preparation. Validation of the HPLC-DAD method showed good linearity (r2>0.997) of the four compounds analyzed in relatively wide concentration ranges. The R.S.D. values for intra-day and inter-day precision were less than 9.0% and the limits of detection (LOD) were less than 22ng. The mean recovery of each compound was 90.2-116.9%. In addition, analysis of ginsenosides Rb1, Rg1, saikosaponins a, b2, and c were established by HPLC-MS in selected ion monitoring (SIM) mode, due to their low sensitivity in HPLC-DAD. These results suggest that the developed analytical method is simple and effective, and could be readily utilized as a quality control method for commercial SGT products.
| Original language | English |
|---|---|
| Pages (from-to) | 2122-2133 |
| Number of pages | 12 |
| Journal | Journal of Liquid Chromatography and Related Technologies |
| Volume | 32 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2009 Sep 25 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Pharmaceutical Science
- Clinical Biochemistry
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Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS. / Lee, Mi Kyeong; Kim, Seung Hyun; Park, Jin Ho; Cho, Jung Hee; Kim, Do Hoon; Baek, Ju Hyun; Cho, Chang Hee; Kim, Hyo Jin; Ahn, Mi Jeong; Lee, Ki Yong; Sung, Sang Hyun.
In: Journal of Liquid Chromatography and Related Technologies, Vol. 32, No. 14, 25.09.2009, p. 2122-2133.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS
AU - Lee, Mi Kyeong
AU - Kim, Seung Hyun
AU - Park, Jin Ho
AU - Cho, Jung Hee
AU - Kim, Do Hoon
AU - Baek, Ju Hyun
AU - Cho, Chang Hee
AU - Kim, Hyo Jin
AU - Ahn, Mi Jeong
AU - Lee, Ki Yong
AU - Sung, Sang Hyun
PY - 2009/9/25
Y1 - 2009/9/25
N2 - Simultaneous determination of four major constituents, paeoniflorin, baicalin, trans-cinnamic acid, and glycyrrhizin in Siho-Gyeoji-Tang (SGT) was established by an HPLC-DAD method for the quality control of traditional herbal medicinal preparation. Validation of the HPLC-DAD method showed good linearity (r2>0.997) of the four compounds analyzed in relatively wide concentration ranges. The R.S.D. values for intra-day and inter-day precision were less than 9.0% and the limits of detection (LOD) were less than 22ng. The mean recovery of each compound was 90.2-116.9%. In addition, analysis of ginsenosides Rb1, Rg1, saikosaponins a, b2, and c were established by HPLC-MS in selected ion monitoring (SIM) mode, due to their low sensitivity in HPLC-DAD. These results suggest that the developed analytical method is simple and effective, and could be readily utilized as a quality control method for commercial SGT products.
AB - Simultaneous determination of four major constituents, paeoniflorin, baicalin, trans-cinnamic acid, and glycyrrhizin in Siho-Gyeoji-Tang (SGT) was established by an HPLC-DAD method for the quality control of traditional herbal medicinal preparation. Validation of the HPLC-DAD method showed good linearity (r2>0.997) of the four compounds analyzed in relatively wide concentration ranges. The R.S.D. values for intra-day and inter-day precision were less than 9.0% and the limits of detection (LOD) were less than 22ng. The mean recovery of each compound was 90.2-116.9%. In addition, analysis of ginsenosides Rb1, Rg1, saikosaponins a, b2, and c were established by HPLC-MS in selected ion monitoring (SIM) mode, due to their low sensitivity in HPLC-DAD. These results suggest that the developed analytical method is simple and effective, and could be readily utilized as a quality control method for commercial SGT products.
UR - http://www.scopus.com/inward/record.url?scp=70349301650&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349301650&partnerID=8YFLogxK
U2 - 10.1080/10826070903127011
DO - 10.1080/10826070903127011
M3 - Article
AN - SCOPUS:70349301650
VL - 32
SP - 2122
EP - 2133
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
SN - 1082-6076
IS - 14
ER -