Determination and identification of nine constituents in Siho-Gyeoji-Tang by HPLC-DAD and HPLC-MS/MS

Mi Kyeong Lee, Seung Hyun Kim, Jin Ho Park, Jung Hee Cho, Do Hoon Kim, Ju Hyun Baek, Chang Hee Cho, Hyo Jin Kim, Mi Jeong Ahn, Ki Yong Lee, Sang Hyun Sung

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Simultaneous determination of four major constituents, paeoniflorin, baicalin, trans-cinnamic acid, and glycyrrhizin in Siho-Gyeoji-Tang (SGT) was established by an HPLC-DAD method for the quality control of traditional herbal medicinal preparation. Validation of the HPLC-DAD method showed good linearity (r2>0.997) of the four compounds analyzed in relatively wide concentration ranges. The R.S.D. values for intra-day and inter-day precision were less than 9.0% and the limits of detection (LOD) were less than 22ng. The mean recovery of each compound was 90.2-116.9%. In addition, analysis of ginsenosides Rb1, Rg1, saikosaponins a, b2, and c were established by HPLC-MS in selected ion monitoring (SIM) mode, due to their low sensitivity in HPLC-DAD. These results suggest that the developed analytical method is simple and effective, and could be readily utilized as a quality control method for commercial SGT products.

Original languageEnglish
Pages (from-to)2122-2133
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Volume32
Issue number14
DOIs
Publication statusPublished - 2009

Bibliographical note

Funding Information:
This research was supported by a grant of the Korea Food & Drug Administration, Republic of Korea (2005).

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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