Determination of a favorable medium for detection of fungal extracellular protease

Jong Chul Park; Dong Wook Han; Yu Shik Hwang; Hun Jun Lee; Kosuke Takatori; Hwal Suh

doi:10.4265/bio.4.91

Determination of a favorable medium for detection of fungal extracellular protease

Jong Chul Park, Dong Wook Han, Yu Shik Hwang, Hun Jun Lee, Kosuke Takatori, Hwal Suh

Department of Medical Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.

Original language	English
Pages (from-to)	91-95
Number of pages	5
Journal	Biocontrol Science
Volume	4
Issue number	2
DOIs	https://doi.org/10.4265/bio.4.91
Publication status	Published - 1999 Sep 20

All Science Journal Classification (ASJC) codes

Applied Microbiology and Biotechnology
Public Health, Environmental and Occupational Health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4265/bio.4.91

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abstract = "To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.",

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AU - Park, Jong Chul

AU - Han, Dong Wook

AU - Hwang, Yu Shik

AU - Lee, Hun Jun

AU - Takatori, Kosuke

AU - Suh, Hwal

PY - 1999/9/20

Y1 - 1999/9/20

N2 - To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.

AB - To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.

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Determination of a favorable medium for detection of fungal extracellular protease

Abstract

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UN SDGs

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