Determination of a favorable medium for detection of fungal extracellular protease

Jong Chul Park; Dong Wook Han; Yu Shik Hwang; Hun Jun Lee; Kosuke Takatori; Hwal Suh

doi:10.4265/bio.4.91

Determination of a favorable medium for detection of fungal extracellular protease

Jong Chul Park, Dong Wook Han, Yu Shik Hwang, Hun Jun Lee, Kosuke Takatori, Hwal Suh

Department of Medical Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.

Original language	English
Pages (from-to)	91-95
Number of pages	5
Journal	Biocontrol Science
Volume	4
Issue number	2
DOIs	https://doi.org/10.4265/bio.4.91
Publication status	Published - 1999 Sep 20

All Science Journal Classification (ASJC) codes

Applied Microbiology and Biotechnology
Public Health, Environmental and Occupational Health

UN SDGs

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abstract = "To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.",

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AU - Park, Jong Chul

AU - Han, Dong Wook

AU - Hwang, Yu Shik

AU - Lee, Hun Jun

AU - Takatori, Kosuke

AU - Suh, Hwal

PY - 1999/9/20

Y1 - 1999/9/20

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Determination of a favorable medium for detection of fungal extracellular protease

Abstract

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