Determination of a favorable medium for detection of fungal extracellular protease

Jongchul Park, Dong Wook Han, Yu Shik Hwang, Hun Jun Lee, Kosuke Takatori, Hwal Suh

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3 Citations (Scopus)

Abstract

To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.

Original languageEnglish
Pages (from-to)91-95
Number of pages5
JournalBiocontrol Science
Volume4
Issue number2
DOIs
Publication statusPublished - 1999 Sep 20

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All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

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