Abstract
To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.
| Original language | English |
|---|---|
| Pages (from-to) | 91-95 |
| Number of pages | 5 |
| Journal | Biocontrol Science |
| Volume | 4 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1999 Sep 20 |
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All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology
- Public Health, Environmental and Occupational Health
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Determination of a favorable medium for detection of fungal extracellular protease. / Park, Jongchul; Han, Dong Wook; Hwang, Yu Shik; Lee, Hun Jun; Takatori, Kosuke; Suh, Hwal.
In: Biocontrol Science, Vol. 4, No. 2, 20.09.1999, p. 91-95.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination of a favorable medium for detection of fungal extracellular protease
AU - Park, Jongchul
AU - Han, Dong Wook
AU - Hwang, Yu Shik
AU - Lee, Hun Jun
AU - Takatori, Kosuke
AU - Suh, Hwal
PY - 1999/9/20
Y1 - 1999/9/20
N2 - To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.
AB - To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X- 100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.
UR - http://www.scopus.com/inward/record.url?scp=0032835020&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032835020&partnerID=8YFLogxK
U2 - 10.4265/bio.4.91
DO - 10.4265/bio.4.91
M3 - Article
AN - SCOPUS:0032835020
VL - 4
SP - 91
EP - 95
JO - Biocontrol Science
JF - Biocontrol Science
SN - 1342-4815
IS - 2
ER -