Determination of aloesin in rat plasma using a column-switching high-performance liquid chromatographic assay

Minsun Baek, Jin Hyun Jeong, Dong Hyun Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A column-switching high-performance liquid chromatography (HPLC) method for the determination of aloesin in rat plasma using column-switching and ultraviolet (UV) absorbance detection is described. Plasma was directly injected onto the HPLC system consisting of a clean-up column, a concentrating column, and an analytical column, which were connected with a six-port switching valve. The determination of aloesin was accurate and repeatable, with a limit of quantitation of 10 ng/ml in plasma. The standard calibration curve for aloesin was linear (r=0.998) over the concentration range of 10-1000 ng/ml in rat plasma. The intra- and inter-day assay variabilities of aloesin ranged from 1.0 to 4.7% and 1.1 to 8.8%, respectively. This highly sensitive and simple method has been successfully applied to a pharmacokinetic study after oral administration of aloesin to rats.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume754
Issue number1
DOIs
Publication statusPublished - 2001 Apr 15

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Rats
Assays
Plasmas
Liquids
High performance liquid chromatography
Pharmacokinetics
aloesin
Calibration

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

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title = "Determination of aloesin in rat plasma using a column-switching high-performance liquid chromatographic assay",
abstract = "A column-switching high-performance liquid chromatography (HPLC) method for the determination of aloesin in rat plasma using column-switching and ultraviolet (UV) absorbance detection is described. Plasma was directly injected onto the HPLC system consisting of a clean-up column, a concentrating column, and an analytical column, which were connected with a six-port switching valve. The determination of aloesin was accurate and repeatable, with a limit of quantitation of 10 ng/ml in plasma. The standard calibration curve for aloesin was linear (r=0.998) over the concentration range of 10-1000 ng/ml in rat plasma. The intra- and inter-day assay variabilities of aloesin ranged from 1.0 to 4.7{\%} and 1.1 to 8.8{\%}, respectively. This highly sensitive and simple method has been successfully applied to a pharmacokinetic study after oral administration of aloesin to rats.",
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Determination of aloesin in rat plasma using a column-switching high-performance liquid chromatographic assay. / Baek, Minsun; Hyun Jeong, Jin; Kim, Dong Hyun.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 754, No. 1, 15.04.2001, p. 121-126.

Research output: Contribution to journalArticle

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