Determination of bevantolol in human plasma by high performance liquid chromatography using solid phase extraction technique

Quoc Trung Tran, Hai Long Pham, Ahmed M. Al-Abd, Jeong Ku Hyo, Yoon Lee Ho, Joo Hwang Sung, Ho Kim Kyeong

Research output: Contribution to journalArticle

Abstract

A method was developed and fully validated for the determination of bevantolol, an adrenergic-receptor blocker, in human plasma. Bevantolol and betaxolol as internal standard (I.S) were extracted from 1mL of human plasma by solid phase extraction technique using Sep-pak silica cartridge. Chromatographic separation was accomplished under isocratic conditions using a reverse-phase C8 analytical column and mixture of dibasic ammonium phosphate (pH 5.7; 50mM)-acetonitrile (75:25, v/v) as mobile phase, with a detection wavelength at 220 nm. The method was proved to be specific by testing six different human plasma sources. Linearity was established for the concentration ranges of 40-1600 ng/mL with correlation coefficent of 0.9995. The lower limit of quantification 40 ng/mL with precision of 10.9% as C.V%.

Original languageEnglish
Pages (from-to)890-897
Number of pages8
JournalArchives of pharmacal research
Volume30
Issue number7
Publication statusPublished - 2007 Jul 31

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Plasma (human)
Solid Phase Extraction
High performance liquid chromatography
Betaxolol
High Pressure Liquid Chromatography
Plasma sources
Silicon Dioxide
Adrenergic Receptors
Adrenergic Antagonists
Wavelength
Testing
bevantolol
ammonium phosphate
acetonitrile

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

Cite this

Tran, Quoc Trung ; Pham, Hai Long ; Al-Abd, Ahmed M. ; Hyo, Jeong Ku ; Ho, Yoon Lee ; Sung, Joo Hwang ; Kyeong, Ho Kim. / Determination of bevantolol in human plasma by high performance liquid chromatography using solid phase extraction technique. In: Archives of pharmacal research. 2007 ; Vol. 30, No. 7. pp. 890-897.
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Determination of bevantolol in human plasma by high performance liquid chromatography using solid phase extraction technique. / Tran, Quoc Trung; Pham, Hai Long; Al-Abd, Ahmed M.; Hyo, Jeong Ku; Ho, Yoon Lee; Sung, Joo Hwang; Kyeong, Ho Kim.

In: Archives of pharmacal research, Vol. 30, No. 7, 31.07.2007, p. 890-897.

Research output: Contribution to journalArticle

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