Determination of bevantolol in human plasma by high performance liquid chromatography using solid phase extraction technique

Quoc Trung Tran, Hai Long Pham, Ahmed M. Al-Abd, Jeong Ku Hyo, Yoon Lee Ho, Joo Hwang Sung, Ho Kim Kyeong

Research output: Contribution to journalArticle

Abstract

A method was developed and fully validated for the determination of bevantolol, an adrenergic-receptor blocker, in human plasma. Bevantolol and betaxolol as internal standard (I.S) were extracted from 1mL of human plasma by solid phase extraction technique using Sep-pak silica cartridge. Chromatographic separation was accomplished under isocratic conditions using a reverse-phase C8 analytical column and mixture of dibasic ammonium phosphate (pH 5.7; 50mM)-acetonitrile (75:25, v/v) as mobile phase, with a detection wavelength at 220 nm. The method was proved to be specific by testing six different human plasma sources. Linearity was established for the concentration ranges of 40-1600 ng/mL with correlation coefficent of 0.9995. The lower limit of quantification 40 ng/mL with precision of 10.9% as C.V%.

Original languageEnglish
Pages (from-to)890-897
Number of pages8
JournalArchives of pharmacal research
Volume30
Issue number7
Publication statusPublished - 2007 Jul 31

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

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