Determination of cyclodextrin content using periodate oxidation by capillary electrophoresis

Martin Pumera, Ivan Jelínek, Jindřich Jindřich, Pavel Coufal, Jirí Horský

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A simple and general method suitable for the determination of cyclodextrin content in various matrices is described. The proposed method involves selective cleavage of C-C bonds with vicinal hydroxyl groups by means of periodate (Malaprade's reaction). The amount of produced iodate is monitored by capillary electrophoresis. Optimized electrophoretic conditions (20 mM disodium tetraborate with 1 mM tetradecyltrimethylammonium bromide, direct UV detection λ = 200 nm) ensure complete separation of periodate and iodate ions and sufficient sensitivity towards iodate. Under optimized reaction conditions (2-fold excess of periodate, temperature 70°C) reproducible quantitative results were obtained for α-, β- and γ- cyclodextrins as model samples. The proposed method was tested on a real sample of acrylamide - 2(I)-O-allyl-β-CD copolymer. The values of β- cyclodextrin content were compared with those obtained by reference NMR measurement and were found to be identical. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalJournal of Chromatography A
Volume891
Issue number1
DOIs
Publication statusPublished - 2000 Sep 1

Fingerprint

Iodates
Capillary electrophoresis
Cyclodextrins
Capillary Electrophoresis
Oxidation
Acrylamide
Hydroxyl Radical
Copolymers
Nuclear magnetic resonance
Ions
Temperature
metaperiodate

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Pumera, Martin ; Jelínek, Ivan ; Jindřich, Jindřich ; Coufal, Pavel ; Horský, Jirí. / Determination of cyclodextrin content using periodate oxidation by capillary electrophoresis. In: Journal of Chromatography A. 2000 ; Vol. 891, No. 1. pp. 201-206.
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Determination of cyclodextrin content using periodate oxidation by capillary electrophoresis. / Pumera, Martin; Jelínek, Ivan; Jindřich, Jindřich; Coufal, Pavel; Horský, Jirí.

In: Journal of Chromatography A, Vol. 891, No. 1, 01.09.2000, p. 201-206.

Research output: Contribution to journalArticle

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