Determination of lamotrigine in human serum by high-performance liquid chromatography-tandem mass spectrometry

Woonhyoung Lee, Jeong Ho Kim, Hyonsuk Kim, Oh Hun Kwon, Byung In Lee, Kyoung Heo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrile as mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1-109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.

Original languageEnglish
Pages (from-to)717-720
Number of pages4
JournalNeurological Sciences
Volume31
Issue number6
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Tandem Mass Spectrometry
High Pressure Liquid Chromatography
Serum
Liquid Chromatography
Ions
Proteins
acetonitrile
lamotrigine
cellulose sulfate

All Science Journal Classification (ASJC) codes

  • Dermatology
  • Clinical Neurology
  • Psychiatry and Mental health

Cite this

Lee, Woonhyoung ; Kim, Jeong Ho ; Kim, Hyonsuk ; Kwon, Oh Hun ; Lee, Byung In ; Heo, Kyoung. / Determination of lamotrigine in human serum by high-performance liquid chromatography-tandem mass spectrometry. In: Neurological Sciences. 2010 ; Vol. 31, No. 6. pp. 717-720.
@article{948915d7985b493b80f32a436a45539a,
title = "Determination of lamotrigine in human serum by high-performance liquid chromatography-tandem mass spectrometry",
abstract = "A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70{\%} acetonitrile as mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1-109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.",
author = "Woonhyoung Lee and Kim, {Jeong Ho} and Hyonsuk Kim and Kwon, {Oh Hun} and Lee, {Byung In} and Kyoung Heo",
year = "2010",
month = "12",
day = "1",
doi = "10.1007/s10072-010-0257-x",
language = "English",
volume = "31",
pages = "717--720",
journal = "Neurological Sciences",
issn = "1590-1874",
publisher = "Springer-Verlag Italia",
number = "6",

}

Determination of lamotrigine in human serum by high-performance liquid chromatography-tandem mass spectrometry. / Lee, Woonhyoung; Kim, Jeong Ho; Kim, Hyonsuk; Kwon, Oh Hun; Lee, Byung In; Heo, Kyoung.

In: Neurological Sciences, Vol. 31, No. 6, 01.12.2010, p. 717-720.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Determination of lamotrigine in human serum by high-performance liquid chromatography-tandem mass spectrometry

AU - Lee, Woonhyoung

AU - Kim, Jeong Ho

AU - Kim, Hyonsuk

AU - Kwon, Oh Hun

AU - Lee, Byung In

AU - Heo, Kyoung

PY - 2010/12/1

Y1 - 2010/12/1

N2 - A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrile as mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1-109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.

AB - A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrile as mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1-109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.

UR - http://www.scopus.com/inward/record.url?scp=78650513509&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650513509&partnerID=8YFLogxK

U2 - 10.1007/s10072-010-0257-x

DO - 10.1007/s10072-010-0257-x

M3 - Article

VL - 31

SP - 717

EP - 720

JO - Neurological Sciences

JF - Neurological Sciences

SN - 1590-1874

IS - 6

ER -