Determination of lamotrigine in human serum by high-performance liquid chromatography-tandem mass spectrometry

Woonhyoung Lee, Jeong Ho Kim, Hyon Suk Kim, Oh Hun Kwon, Byung In Lee, Kyoung Heo

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A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrile as mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1-109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.

Original languageEnglish
Pages (from-to)717-720
Number of pages4
JournalNeurological Sciences
Issue number6
Publication statusPublished - 2010 Dec 1


All Science Journal Classification (ASJC) codes

  • Dermatology
  • Clinical Neurology
  • Psychiatry and Mental health

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