Abstract
A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrile as mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1-109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.
| Original language | English |
|---|---|
| Pages (from-to) | 717-720 |
| Number of pages | 4 |
| Journal | Neurological Sciences |
| Volume | 31 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2010 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Dermatology
- Clinical Neurology
- Psychiatry and Mental health
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Determination of lamotrigine in human serum by high-performance liquid chromatography-tandem mass spectrometry. / Lee, Woonhyoung; Kim, Jeong Ho; Kim, Hyonsuk; Kwon, Oh Hun; Lee, Byung In; Heo, Kyoung.
In: Neurological Sciences, Vol. 31, No. 6, 01.12.2010, p. 717-720.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination of lamotrigine in human serum by high-performance liquid chromatography-tandem mass spectrometry
AU - Lee, Woonhyoung
AU - Kim, Jeong Ho
AU - Kim, Hyonsuk
AU - Kwon, Oh Hun
AU - Lee, Byung In
AU - Heo, Kyoung
PY - 2010/12/1
Y1 - 2010/12/1
N2 - A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrile as mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1-109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.
AB - A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of lamotrigine in human serum. After a simple protein precipitation using acetonitrile, the analytes were separated on a Shideido 150 mm × 2.0 mm, 5 μm Capcell Pak C18 MG column using 70% acetonitrile as mobile phase at a flow rate of 200 μl/min. Lamotrigine was eluted at 1.98 min, ionized using electrospray ionization source, and then detected by multiple reaction monitoring mode. The precursor to product ion transitions of m/z 256.1-109.0 was used to quantify. The analytical measurement range is from 0.1 to 20 μg/ml and the upper clinical reportable range is chosen to be 100 μg/ml. The method was successfully employed in a clinical application.
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U2 - 10.1007/s10072-010-0257-x
DO - 10.1007/s10072-010-0257-x
M3 - Article
VL - 31
SP - 717
EP - 720
JO - Neurological Sciences
JF - Neurological Sciences
SN - 1590-1874
IS - 6
ER -