Determination of optimal glycerol concentration for optical tissue clearing

Eungjun Youn, Taeyoon Son, Han Sung Kim, Byungjo Jung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

The laser scattering in tissue is significant in diagnostic and therapeutic purposes of laser. Many studies have been conducted to minimize laser scattering in tissue and therefore, to maximize the clinical efficacy by enhancing photon density. Optical clearing agents (OCAs) have been employed for optical tissue clearing (OTC). This study was aimed to investigate the optimal concentration of an OCA, glycerol, in topical application, so that it can be utilized for clinical diagnosis and therapy in dermatology. Glycerol was topically applied to avoid possible edema caused by dermal injection. The effect of OTC was quantitatively evaluated as a function of the concentration of glycerol with various methods. Optical methods such as optical coherence tomography (OCT) and an integrating sphere were used to assess the enhancement of light penetration depth and refractive index matching. In addition, a non-optical method, ultrasound scanner, was utilized to evaluate quantitatively collagen dissociation. The results revealed that 70 % glycerol was the optimal concentration of OTC for topical application. This study may provide a guideline regarding to the use of glycerol for optimal diagnostic and therapeutic effects in dermatology.

Original languageEnglish
Title of host publicationPhotonic Therapeutics and Diagnostics VIII
Volume8207
DOIs
Publication statusPublished - 2012 May 14
EventPhotonic Therapeutics and Diagnostics VIII - San Francisco, CA, United States
Duration: 2012 Jan 212012 Jan 24

Other

OtherPhotonic Therapeutics and Diagnostics VIII
CountryUnited States
CitySan Francisco, CA
Period12/1/2112/1/24

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Youn, E., Son, T., Kim, H. S., & Jung, B. (2012). Determination of optimal glycerol concentration for optical tissue clearing. In Photonic Therapeutics and Diagnostics VIII (Vol. 8207). [82070J] https://doi.org/10.1117/12.909790