Abstract
Although panduratin A, a chalcone derivative isolated from the rhizome of Kaempferia pandurata Roxb., represents a variety of pharmacological activities, no validated method for determination of panduratin A levels in biological samples is yet available. Thus, we developed a liquid chromatographic method using a tandem mass spectrometry for the determination of panduratin A in rat plasma. After simple protein precipitation with methanol including flufenamic acid (internal standard, IS), the analytes were chromatographed on a reversed-phased column with a mobile phase of distilled water and acetonitrile including 2% of formic acid (2:8, v/v). The ion transitions of the precursor to the product ion were principally deprotonated ions [M−H] − at m/z 405.2 → 165.9 for panduratin A and 280.1 → 236.0 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor the plasma concentrations of panduratin A following oral administration in rats.
| Original language | English |
|---|---|
| Pages (from-to) | 151-154 |
| Number of pages | 4 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 137 |
| DOIs | |
| Publication status | Published - 2017 Apr 15 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Pharmaceutical Science
- Drug Discovery
- Spectroscopy
- Clinical Biochemistry
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Determination of panduratin A in rat plasma by HPLC–MS/MS and its application to a pharmacokinetic study. / Kim, Minsoo; Choi, Seungmok; Noh, Keumhan; Kim, Changhee; Kim, Eunyoung; Hwang, Jae-Kwan; Kang, Wonku.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 137, 15.04.2017, p. 151-154.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination of panduratin A in rat plasma by HPLC–MS/MS and its application to a pharmacokinetic study
AU - Kim, Minsoo
AU - Choi, Seungmok
AU - Noh, Keumhan
AU - Kim, Changhee
AU - Kim, Eunyoung
AU - Hwang, Jae-Kwan
AU - Kang, Wonku
PY - 2017/4/15
Y1 - 2017/4/15
N2 - Although panduratin A, a chalcone derivative isolated from the rhizome of Kaempferia pandurata Roxb., represents a variety of pharmacological activities, no validated method for determination of panduratin A levels in biological samples is yet available. Thus, we developed a liquid chromatographic method using a tandem mass spectrometry for the determination of panduratin A in rat plasma. After simple protein precipitation with methanol including flufenamic acid (internal standard, IS), the analytes were chromatographed on a reversed-phased column with a mobile phase of distilled water and acetonitrile including 2% of formic acid (2:8, v/v). The ion transitions of the precursor to the product ion were principally deprotonated ions [M−H] − at m/z 405.2 → 165.9 for panduratin A and 280.1 → 236.0 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor the plasma concentrations of panduratin A following oral administration in rats.
AB - Although panduratin A, a chalcone derivative isolated from the rhizome of Kaempferia pandurata Roxb., represents a variety of pharmacological activities, no validated method for determination of panduratin A levels in biological samples is yet available. Thus, we developed a liquid chromatographic method using a tandem mass spectrometry for the determination of panduratin A in rat plasma. After simple protein precipitation with methanol including flufenamic acid (internal standard, IS), the analytes were chromatographed on a reversed-phased column with a mobile phase of distilled water and acetonitrile including 2% of formic acid (2:8, v/v). The ion transitions of the precursor to the product ion were principally deprotonated ions [M−H] − at m/z 405.2 → 165.9 for panduratin A and 280.1 → 236.0 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor the plasma concentrations of panduratin A following oral administration in rats.
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U2 - 10.1016/j.jpba.2017.01.027
DO - 10.1016/j.jpba.2017.01.027
M3 - Article
VL - 137
SP - 151
EP - 154
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
ER -