Determination of panduratin A in rat plasma by HPLC–MS/MS and its application to a pharmacokinetic study

Minsoo Kim, Seungmok Choi, Keumhan Noh, Changhee Kim, Eunyoung Kim, Jae-Kwan Hwang, Wonku Kang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Although panduratin A, a chalcone derivative isolated from the rhizome of Kaempferia pandurata Roxb., represents a variety of pharmacological activities, no validated method for determination of panduratin A levels in biological samples is yet available. Thus, we developed a liquid chromatographic method using a tandem mass spectrometry for the determination of panduratin A in rat plasma. After simple protein precipitation with methanol including flufenamic acid (internal standard, IS), the analytes were chromatographed on a reversed-phased column with a mobile phase of distilled water and acetonitrile including 2% of formic acid (2:8, v/v). The ion transitions of the precursor to the product ion were principally deprotonated ions [M−H] at m/z 405.2 → 165.9 for panduratin A and 280.1 → 236.0 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor the plasma concentrations of panduratin A following oral administration in rats.

Original languageEnglish
Pages (from-to)151-154
Number of pages4
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume137
DOIs
Publication statusPublished - 2017 Apr 15

Fingerprint

Pharmacokinetics
Rats
Plasmas
formic acid
Ions
Flufenamic Acid
Zingiberaceae
Chalcone
Rhizome
Tandem Mass Spectrometry
Mass spectrometry
Oral Administration
Methanol
Assays
panduratin A
Pharmacology
Derivatives
Water
Liquids
Proteins

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Kim, Minsoo ; Choi, Seungmok ; Noh, Keumhan ; Kim, Changhee ; Kim, Eunyoung ; Hwang, Jae-Kwan ; Kang, Wonku. / Determination of panduratin A in rat plasma by HPLC–MS/MS and its application to a pharmacokinetic study. In: Journal of Pharmaceutical and Biomedical Analysis. 2017 ; Vol. 137. pp. 151-154.
@article{28b9143e671d4a158511d1dfc84eb864,
title = "Determination of panduratin A in rat plasma by HPLC–MS/MS and its application to a pharmacokinetic study",
abstract = "Although panduratin A, a chalcone derivative isolated from the rhizome of Kaempferia pandurata Roxb., represents a variety of pharmacological activities, no validated method for determination of panduratin A levels in biological samples is yet available. Thus, we developed a liquid chromatographic method using a tandem mass spectrometry for the determination of panduratin A in rat plasma. After simple protein precipitation with methanol including flufenamic acid (internal standard, IS), the analytes were chromatographed on a reversed-phased column with a mobile phase of distilled water and acetonitrile including 2{\%} of formic acid (2:8, v/v). The ion transitions of the precursor to the product ion were principally deprotonated ions [M−H] − at m/z 405.2 → 165.9 for panduratin A and 280.1 → 236.0 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor the plasma concentrations of panduratin A following oral administration in rats.",
author = "Minsoo Kim and Seungmok Choi and Keumhan Noh and Changhee Kim and Eunyoung Kim and Jae-Kwan Hwang and Wonku Kang",
year = "2017",
month = "4",
day = "15",
doi = "10.1016/j.jpba.2017.01.027",
language = "English",
volume = "137",
pages = "151--154",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",

}

Determination of panduratin A in rat plasma by HPLC–MS/MS and its application to a pharmacokinetic study. / Kim, Minsoo; Choi, Seungmok; Noh, Keumhan; Kim, Changhee; Kim, Eunyoung; Hwang, Jae-Kwan; Kang, Wonku.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 137, 15.04.2017, p. 151-154.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Determination of panduratin A in rat plasma by HPLC–MS/MS and its application to a pharmacokinetic study

AU - Kim, Minsoo

AU - Choi, Seungmok

AU - Noh, Keumhan

AU - Kim, Changhee

AU - Kim, Eunyoung

AU - Hwang, Jae-Kwan

AU - Kang, Wonku

PY - 2017/4/15

Y1 - 2017/4/15

N2 - Although panduratin A, a chalcone derivative isolated from the rhizome of Kaempferia pandurata Roxb., represents a variety of pharmacological activities, no validated method for determination of panduratin A levels in biological samples is yet available. Thus, we developed a liquid chromatographic method using a tandem mass spectrometry for the determination of panduratin A in rat plasma. After simple protein precipitation with methanol including flufenamic acid (internal standard, IS), the analytes were chromatographed on a reversed-phased column with a mobile phase of distilled water and acetonitrile including 2% of formic acid (2:8, v/v). The ion transitions of the precursor to the product ion were principally deprotonated ions [M−H] − at m/z 405.2 → 165.9 for panduratin A and 280.1 → 236.0 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor the plasma concentrations of panduratin A following oral administration in rats.

AB - Although panduratin A, a chalcone derivative isolated from the rhizome of Kaempferia pandurata Roxb., represents a variety of pharmacological activities, no validated method for determination of panduratin A levels in biological samples is yet available. Thus, we developed a liquid chromatographic method using a tandem mass spectrometry for the determination of panduratin A in rat plasma. After simple protein precipitation with methanol including flufenamic acid (internal standard, IS), the analytes were chromatographed on a reversed-phased column with a mobile phase of distilled water and acetonitrile including 2% of formic acid (2:8, v/v). The ion transitions of the precursor to the product ion were principally deprotonated ions [M−H] − at m/z 405.2 → 165.9 for panduratin A and 280.1 → 236.0 for the IS. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor the plasma concentrations of panduratin A following oral administration in rats.

UR - http://www.scopus.com/inward/record.url?scp=85009911238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85009911238&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2017.01.027

DO - 10.1016/j.jpba.2017.01.027

M3 - Article

VL - 137

SP - 151

EP - 154

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -