Determination of phenothiazine drugs using tris(2,2'-bipyridyl) ruthenium(II) electrogenerated chemiluminescence at DNA-modified electrode

Ra Young Hwang, Guang Ri Xu, Jaeyong Han, Ja Young Lee, Han Nim Choi, Wonyong Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

DNA-modified electrode has been used for the highly selective and sensitive Ru(bpy)3 2+ electrogenerated chemiluminescent determination of phenothiazine drugs in urine sample. Double-stranded calf-thymus DNA was immobilized by covalently bonding to a conducting polymer containing a carboxylic group, which was electrochemically polymerized on a glassy carbon electrode with a monomer of thiophene-3-acetic acid. Phenothiazine derivatives were selectively preconcentrated onto the DNA-modified electrode due to their intercalative binding properties with DNA double helix. The phenothiazine derivatives as coreactants were then sensitively detected with the Ru(bpy)3 2+ electrogenerated chemiluminescence. The present method can detect phenothiazine drugs in the linear range of 1.0 × 10-8 M-1.0 × 10-5 M with detection limits of nanomolar concentration level. Moreover, the interference from ascorbic acid, glutamic acid, and amino acids was almost negligible because of electrostatic repulsion between the negatively charged DNA-modified electrode and anionic interfering species. The present detection method exhibited 95.2% recovery in human urine sample. Therefore, the present method can be applicable for real sample analysis.

Original languageEnglish
Pages (from-to)258-263
Number of pages6
JournalJournal of Electroanalytical Chemistry
Volume656
Issue number1-2
DOIs
Publication statusPublished - 2011 Jun 15

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Chemiluminescence
Ruthenium
DNA
Electrodes
Pharmaceutical Preparations
Derivatives
Glassy carbon
Conducting polymers
Thymus
Ascorbic Acid
Ascorbic acid
Glutamic Acid
Thiophene
Electrostatics
Acetic acid
Monomers
Amino acids
Amino Acids
Recovery
tris(2,2'-bipyridyl)ruthenium(II)

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

Cite this

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title = "Determination of phenothiazine drugs using tris(2,2'-bipyridyl) ruthenium(II) electrogenerated chemiluminescence at DNA-modified electrode",
abstract = "DNA-modified electrode has been used for the highly selective and sensitive Ru(bpy)3 2+ electrogenerated chemiluminescent determination of phenothiazine drugs in urine sample. Double-stranded calf-thymus DNA was immobilized by covalently bonding to a conducting polymer containing a carboxylic group, which was electrochemically polymerized on a glassy carbon electrode with a monomer of thiophene-3-acetic acid. Phenothiazine derivatives were selectively preconcentrated onto the DNA-modified electrode due to their intercalative binding properties with DNA double helix. The phenothiazine derivatives as coreactants were then sensitively detected with the Ru(bpy)3 2+ electrogenerated chemiluminescence. The present method can detect phenothiazine drugs in the linear range of 1.0 × 10-8 M-1.0 × 10-5 M with detection limits of nanomolar concentration level. Moreover, the interference from ascorbic acid, glutamic acid, and amino acids was almost negligible because of electrostatic repulsion between the negatively charged DNA-modified electrode and anionic interfering species. The present detection method exhibited 95.2{\%} recovery in human urine sample. Therefore, the present method can be applicable for real sample analysis.",
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Determination of phenothiazine drugs using tris(2,2'-bipyridyl) ruthenium(II) electrogenerated chemiluminescence at DNA-modified electrode. / Hwang, Ra Young; Xu, Guang Ri; Han, Jaeyong; Lee, Ja Young; Choi, Han Nim; Lee, Wonyong.

In: Journal of Electroanalytical Chemistry, Vol. 656, No. 1-2, 15.06.2011, p. 258-263.

Research output: Contribution to journalArticle

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AU - Choi, Han Nim

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