Determination of Saponins and Flavonoids in Ivy leaf extracts using HPLC-DAD

Miao Yu, Young June Shin, Nanyoung Kim, Guijae Yoo, Seon Ju Park, Seung Hyun Kim

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


A new method for the determination of six compounds, chlorogenic acid, rutin, nicotiflorin, hederacoside C, hederasaponin B and α-hederin, in ivy leaf extracts using high-performance liquid chromatography with diode array detector was developed. The chromatographic separation was performed on a YMC Hydrosphere C18 analytical column using a gradient elution of 0.1% phosphoric acid and acetonitrile. The method was validated in terms of specificity, linearity (r2 > 0.9999), precision [relative standard deviation (RSD) < 0.36%] and accuracy (97.4-103.8%). The limit of detection and limit of quantification were <20.32 and 61.56 ng for all analytes, respectively. The tested compounds were found to be stable in the ivy leaf extract from 0 to 48 h, and the RSD value for each compound was <0.90%. The validated method was successfully applied to quantify all six compounds in a 30% ethanol ivy leaf extract and 13 ivy leaf extract products. The results showed that all the tested products satisfied the minimum requirement for the content of hederacoside C. However, there were some differences between the contents of other constituents.

Original languageEnglish
Pages (from-to)478-483
Number of pages6
JournalJournal of Chromatographic Science
Issue number4
Publication statusPublished - 2015 Apr 1

Bibliographical note

Funding Information:
This study was supported by the Global Leading Technology Program of the Office of Strategic R&D Planning (OSP) funded by the Ministry of Knowledge Economy, Republic of Korea (No. 10039320).

Publisher Copyright:
© The Author 2014. Published by Oxford University Press.

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry


Dive into the research topics of 'Determination of Saponins and Flavonoids in Ivy leaf extracts using HPLC-DAD'. Together they form a unique fingerprint.

Cite this