Abstract
Capillary electrophoresis-electrospray tandem mass spectrometry (CE-ESI/ MS/MS) is a simple and highly sensitive method for quantifying seven urinary androgen glucuronides. The urine samples were diluted and filtered through a membrane filter, and the filtrate was injected into a CE-MS/MS system without further sample preparation steps such as extraction and derivatization. The calibration ranges were 0.01-5 μg/ mL for glucuronides of androsterone and 11β-OHAn-3G, and 5-500 ng/ mL for glucuronides of 11-ketoAn, DHEA, testosterone, epitestosterone and DHT. The linearity of the method was 0.992-0.998, and the limits-of-detection at a signal-to-noise ratio of 3 were 5-10 ng/mL. The coefficients of variation were in the range of 4.0-9.0% for intra-day assay and 4.1-9.8% for inter-day assay. The proposed method may be applicable to metabolic profiling in both quantitative and qualitative analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 426-433 |
| Number of pages | 8 |
| Journal | Biomedical Chromatography |
| Volume | 23 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2009 Apr 27 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Molecular Biology
- Pharmacology
- Drug Discovery
- Clinical Biochemistry
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Determination of urinary androgen glucuronides by capillary electrophoresis with electrospray tandem mass spectrometry. / Cho, Sung Hee; Lee, Jeongae; Choi, Man Ho; Lee, Won Yong; Chung, Bong Chul.
In: Biomedical Chromatography, Vol. 23, No. 4, 27.04.2009, p. 426-433.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination of urinary androgen glucuronides by capillary electrophoresis with electrospray tandem mass spectrometry
AU - Cho, Sung Hee
AU - Lee, Jeongae
AU - Choi, Man Ho
AU - Lee, Won Yong
AU - Chung, Bong Chul
PY - 2009/4/27
Y1 - 2009/4/27
N2 - Capillary electrophoresis-electrospray tandem mass spectrometry (CE-ESI/ MS/MS) is a simple and highly sensitive method for quantifying seven urinary androgen glucuronides. The urine samples were diluted and filtered through a membrane filter, and the filtrate was injected into a CE-MS/MS system without further sample preparation steps such as extraction and derivatization. The calibration ranges were 0.01-5 μg/ mL for glucuronides of androsterone and 11β-OHAn-3G, and 5-500 ng/ mL for glucuronides of 11-ketoAn, DHEA, testosterone, epitestosterone and DHT. The linearity of the method was 0.992-0.998, and the limits-of-detection at a signal-to-noise ratio of 3 were 5-10 ng/mL. The coefficients of variation were in the range of 4.0-9.0% for intra-day assay and 4.1-9.8% for inter-day assay. The proposed method may be applicable to metabolic profiling in both quantitative and qualitative analysis.
AB - Capillary electrophoresis-electrospray tandem mass spectrometry (CE-ESI/ MS/MS) is a simple and highly sensitive method for quantifying seven urinary androgen glucuronides. The urine samples were diluted and filtered through a membrane filter, and the filtrate was injected into a CE-MS/MS system without further sample preparation steps such as extraction and derivatization. The calibration ranges were 0.01-5 μg/ mL for glucuronides of androsterone and 11β-OHAn-3G, and 5-500 ng/ mL for glucuronides of 11-ketoAn, DHEA, testosterone, epitestosterone and DHT. The linearity of the method was 0.992-0.998, and the limits-of-detection at a signal-to-noise ratio of 3 were 5-10 ng/mL. The coefficients of variation were in the range of 4.0-9.0% for intra-day assay and 4.1-9.8% for inter-day assay. The proposed method may be applicable to metabolic profiling in both quantitative and qualitative analysis.
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UR - http://www.scopus.com/inward/citedby.url?scp=65249108237&partnerID=8YFLogxK
U2 - 10.1002/bmc.1132
DO - 10.1002/bmc.1132
M3 - Article
C2 - 19101915
AN - SCOPUS:65249108237
VL - 23
SP - 426
EP - 433
JO - Biomedical Chromatography
JF - Biomedical Chromatography
SN - 0269-3879
IS - 4
ER -