Abstract
Capillary electrophoresis-electrospray tandem mass spectrometry (CE-ESI/ MS/MS) is a simple and highly sensitive method for quantifying seven urinary androgen glucuronides. The urine samples were diluted and filtered through a membrane filter, and the filtrate was injected into a CE-MS/MS system without further sample preparation steps such as extraction and derivatization. The calibration ranges were 0.01-5 μg/ mL for glucuronides of androsterone and 11β-OHAn-3G, and 5-500 ng/ mL for glucuronides of 11-ketoAn, DHEA, testosterone, epitestosterone and DHT. The linearity of the method was 0.992-0.998, and the limits-of-detection at a signal-to-noise ratio of 3 were 5-10 ng/mL. The coefficients of variation were in the range of 4.0-9.0% for intra-day assay and 4.1-9.8% for inter-day assay. The proposed method may be applicable to metabolic profiling in both quantitative and qualitative analysis.
Original language | English |
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Pages (from-to) | 426-433 |
Number of pages | 8 |
Journal | Biomedical Chromatography |
Volume | 23 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2009 |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Molecular Biology
- Pharmacology
- Drug Discovery
- Clinical Biochemistry