Developing genetically engineered mouse models using engineered nucleases: Current status, challenges, and the way forward

Jaehoon Lee, Jae il Rho, Sushil Devkota, Young Hoon Sung, Han Woong Lee

Research output: Contribution to journalReview articlepeer-review

2 Citations (Scopus)

Abstract

The rapid development of engineered nucleases such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regulated interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease 9 (Cas9) system has ushered in the era of ‘renaissance in precision genome engineering’ with profound potential to generate mouse models of human diseases. However, with accumulating experience, some drawbacks that we must seriously consider have appeared along with the recent advances in molecular genetics. Here, we highlight recent technical advances of engineered nucleases, discuss the challenges we have faced while using these ‘state of the art’ genome-editing technologies to generate genetically engineered mouse models (GEMs) and, and look toward the potential future uses of these technologies.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalDrug Discovery Today: Disease Models
Volume20
DOIs
Publication statusPublished - 2016 Feb 2

Bibliographical note

Funding Information:
This research was supported by a grant ( 14182MFDS978 ) from Korea Food and Drug Administration in 2015; the National Research Foundation of Korea (NRF) funded by the MEST ( 2015R1A2A1A01003845 ); and a Korean Healthcare Technology R&D Project from the Ministry of Health and Welfare ( A085136 ).

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery

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