Development and characterization of a live attenuated influenza B virus vaccine candidate

Sang Uk Seo, Young Ho Byun, Eun Young Lee, Eun Ju Jung, Yo Han Jang, Hyun Ah Kim, Suk Hoon Ha, Kwang Hee Lee, Baik Lin Seong

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A human influenza B/Lee/40 virus was cold-adapted by serial passages in embryonated chicken eggs, at progressively lower temperatures, for possible use as a future influenza B vaccine donor strain. Temperature sensitive and cold-adapted phenotypes were achieved as a consequence of the adaptation process. It was determined that the virus was attenuated in mice since the replication of the viral genome was significantly reduced in the lung. Despite decreased viral replication, the attenuated infection effectively induced a virus-specific immune response. We next developed a reassortant virus carrying two major surface proteins, hemagglutinin and neuraminidase from virulent B/Shangdong/7/97 and six internal genes from the cold-adapted B/Lee/40. The reassortant virus was also attenuated and protected mice from lethal challenge with wild type B/Shangdong/7/97. In addition, vaccination with the reassortant virus resulted in a specific antibody response and inhibited the replication of wild type virus in mice. We conclude that the cold-adapted B/Lee/40 donor strain merits further investigation as potential live vaccine carrier as an alternative means for protection from influenza B virus epidemics.

Original languageEnglish
Pages (from-to)874-881
Number of pages8
JournalVaccine
Volume26
Issue number7
DOIs
Publication statusPublished - 2008 Feb 13

Fingerprint

Influenza B virus
Reassortant Viruses
Influenza Vaccines
vaccines
viruses
Viruses
Serial Passage
virus replication
Viral Genome
Hemagglutinins
Neuraminidase
Virus Replication
mice
Human Influenza
Eggs
Antibody Formation
Chickens
Membrane Proteins
Vaccination
Vaccines

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Seo, Sang Uk ; Byun, Young Ho ; Lee, Eun Young ; Jung, Eun Ju ; Jang, Yo Han ; Kim, Hyun Ah ; Ha, Suk Hoon ; Lee, Kwang Hee ; Seong, Baik Lin. / Development and characterization of a live attenuated influenza B virus vaccine candidate. In: Vaccine. 2008 ; Vol. 26, No. 7. pp. 874-881.
@article{d144aa20dc3241afae9dd0187cb6a9df,
title = "Development and characterization of a live attenuated influenza B virus vaccine candidate",
abstract = "A human influenza B/Lee/40 virus was cold-adapted by serial passages in embryonated chicken eggs, at progressively lower temperatures, for possible use as a future influenza B vaccine donor strain. Temperature sensitive and cold-adapted phenotypes were achieved as a consequence of the adaptation process. It was determined that the virus was attenuated in mice since the replication of the viral genome was significantly reduced in the lung. Despite decreased viral replication, the attenuated infection effectively induced a virus-specific immune response. We next developed a reassortant virus carrying two major surface proteins, hemagglutinin and neuraminidase from virulent B/Shangdong/7/97 and six internal genes from the cold-adapted B/Lee/40. The reassortant virus was also attenuated and protected mice from lethal challenge with wild type B/Shangdong/7/97. In addition, vaccination with the reassortant virus resulted in a specific antibody response and inhibited the replication of wild type virus in mice. We conclude that the cold-adapted B/Lee/40 donor strain merits further investigation as potential live vaccine carrier as an alternative means for protection from influenza B virus epidemics.",
author = "Seo, {Sang Uk} and Byun, {Young Ho} and Lee, {Eun Young} and Jung, {Eun Ju} and Jang, {Yo Han} and Kim, {Hyun Ah} and Ha, {Suk Hoon} and Lee, {Kwang Hee} and Seong, {Baik Lin}",
year = "2008",
month = "2",
day = "13",
doi = "10.1016/j.vaccine.2007.12.022",
language = "English",
volume = "26",
pages = "874--881",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "7",

}

Seo, SU, Byun, YH, Lee, EY, Jung, EJ, Jang, YH, Kim, HA, Ha, SH, Lee, KH & Seong, BL 2008, 'Development and characterization of a live attenuated influenza B virus vaccine candidate', Vaccine, vol. 26, no. 7, pp. 874-881. https://doi.org/10.1016/j.vaccine.2007.12.022

Development and characterization of a live attenuated influenza B virus vaccine candidate. / Seo, Sang Uk; Byun, Young Ho; Lee, Eun Young; Jung, Eun Ju; Jang, Yo Han; Kim, Hyun Ah; Ha, Suk Hoon; Lee, Kwang Hee; Seong, Baik Lin.

In: Vaccine, Vol. 26, No. 7, 13.02.2008, p. 874-881.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development and characterization of a live attenuated influenza B virus vaccine candidate

AU - Seo, Sang Uk

AU - Byun, Young Ho

AU - Lee, Eun Young

AU - Jung, Eun Ju

AU - Jang, Yo Han

AU - Kim, Hyun Ah

AU - Ha, Suk Hoon

AU - Lee, Kwang Hee

AU - Seong, Baik Lin

PY - 2008/2/13

Y1 - 2008/2/13

N2 - A human influenza B/Lee/40 virus was cold-adapted by serial passages in embryonated chicken eggs, at progressively lower temperatures, for possible use as a future influenza B vaccine donor strain. Temperature sensitive and cold-adapted phenotypes were achieved as a consequence of the adaptation process. It was determined that the virus was attenuated in mice since the replication of the viral genome was significantly reduced in the lung. Despite decreased viral replication, the attenuated infection effectively induced a virus-specific immune response. We next developed a reassortant virus carrying two major surface proteins, hemagglutinin and neuraminidase from virulent B/Shangdong/7/97 and six internal genes from the cold-adapted B/Lee/40. The reassortant virus was also attenuated and protected mice from lethal challenge with wild type B/Shangdong/7/97. In addition, vaccination with the reassortant virus resulted in a specific antibody response and inhibited the replication of wild type virus in mice. We conclude that the cold-adapted B/Lee/40 donor strain merits further investigation as potential live vaccine carrier as an alternative means for protection from influenza B virus epidemics.

AB - A human influenza B/Lee/40 virus was cold-adapted by serial passages in embryonated chicken eggs, at progressively lower temperatures, for possible use as a future influenza B vaccine donor strain. Temperature sensitive and cold-adapted phenotypes were achieved as a consequence of the adaptation process. It was determined that the virus was attenuated in mice since the replication of the viral genome was significantly reduced in the lung. Despite decreased viral replication, the attenuated infection effectively induced a virus-specific immune response. We next developed a reassortant virus carrying two major surface proteins, hemagglutinin and neuraminidase from virulent B/Shangdong/7/97 and six internal genes from the cold-adapted B/Lee/40. The reassortant virus was also attenuated and protected mice from lethal challenge with wild type B/Shangdong/7/97. In addition, vaccination with the reassortant virus resulted in a specific antibody response and inhibited the replication of wild type virus in mice. We conclude that the cold-adapted B/Lee/40 donor strain merits further investigation as potential live vaccine carrier as an alternative means for protection from influenza B virus epidemics.

UR - http://www.scopus.com/inward/record.url?scp=38649133169&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38649133169&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2007.12.022

DO - 10.1016/j.vaccine.2007.12.022

M3 - Article

C2 - 18207290

AN - SCOPUS:38649133169

VL - 26

SP - 874

EP - 881

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 7

ER -