A relatively simple method was developed to fabricate CrN/Cu nanocomposite coatings using pulsed DC magnetron sputtering for application in antibacterial activity. These nanocomposite coatings were applied on titanium (Ti)-modified stainless steel substrata (D-9 alloy) and the antibacterial activity of these coating with respect to the Gram-negative bacterium Pseudomonas aeruginosa was investigated qualitatively and quantitatively. Scanning electron microscopy, epifluorescence microscope analyses, and total viable counts confirmed that inclusion of copper in the CrN/Cu nanocomposite coatings provided antibacterial activity against P. aeruginosa. The quantitative examination of the bacterial activity of P. aeruginosa was estimated by the survival ratio as calculated from the number of viable cells which formed colonies on nutrient agar plates.
Bibliographical noteFunding Information:
The authors thank Dr M. Vijayalakshmi, AD, PMG, Dr T. Jayakumar, Director, MMG and Shri S.C. Chetal, Director, IGCAR, for their encouragement and support. The authors are thankful for the cross-sectional SEM provided by Dr Klaus Ellmer, Abt. Solare Brennstoffe Helmholtz-Zentrum, Berlin. The authors thankfully acknowledge the immense help rendered by Dr P. Meena, PSGRKC, Coimbatore, in preparing this manuscript. This work has been partly supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (No. 2011-0000409).
All Science Journal Classification (ASJC) codes
- Aquatic Science
- Applied Microbiology and Biotechnology
- Water Science and Technology