Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

J. Nylk, M. V.G. Kristensen, M. Mazilu, A. K. Thayil, C. A. Mitchell, E. C. Campbell, S. J. Powis, F. J. Gunn-Moore, K. Dholakia

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8 Citations (Scopus)

Abstract

We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200mm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

Original languageEnglish
Article numberA036
Pages (from-to)1512-1519
Number of pages8
JournalBiomedical Optics Express
Volume6
Issue number4
DOIs
Publication statusPublished - 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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    Nylk, J., Kristensen, M. V. G., Mazilu, M., Thayil, A. K., Mitchell, C. A., Campbell, E. C., Powis, S. J., Gunn-Moore, F. J., & Dholakia, K. (2015). Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis. Biomedical Optics Express, 6(4), 1512-1519. [A036]. https://doi.org/10.1364/BOE.6.001512