Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

J. Nylk, M. V.G. Kristensen, M. Mazilu, A. K. Thayil, C. A. Mitchell, E. C. Campbell, S. J. Powis, F. J. Gunn-Moore, K. Dholakia

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200mm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

Original languageEnglish
Article numberA036
Pages (from-to)1512-1519
Number of pages8
JournalBiomedical Optics Express
Volume6
Issue number4
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Optical Tweezers
principal components analysis
Principal Component Analysis
optical fibers
trapping
Immunological Synapses
traps
probes
Lenses
synapses
Microscopy
numerical aperture
lenses
microscopy
fibers

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Nylk, J. ; Kristensen, M. V.G. ; Mazilu, M. ; Thayil, A. K. ; Mitchell, C. A. ; Campbell, E. C. ; Powis, S. J. ; Gunn-Moore, F. J. ; Dholakia, K. / Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis. In: Biomedical Optics Express. 2015 ; Vol. 6, No. 4. pp. 1512-1519.
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Nylk, J, Kristensen, MVG, Mazilu, M, Thayil, AK, Mitchell, CA, Campbell, EC, Powis, SJ, Gunn-Moore, FJ & Dholakia, K 2015, 'Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis', Biomedical Optics Express, vol. 6, no. 4, A036, pp. 1512-1519. https://doi.org/10.1364/BOE.6.001512

Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis. / Nylk, J.; Kristensen, M. V.G.; Mazilu, M.; Thayil, A. K.; Mitchell, C. A.; Campbell, E. C.; Powis, S. J.; Gunn-Moore, F. J.; Dholakia, K.

In: Biomedical Optics Express, Vol. 6, No. 4, A036, 01.01.2015, p. 1512-1519.

Research output: Contribution to journalArticle

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AU - Mitchell, C. A.

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