Development of a new xenoestrogen screening system using fission yeast Schizosaccharomyces pombe

Eung Jae Yoo, Yeun Kyu Jang, Hyun Soo Kimm, Eun Sik Choi, Sang Dai Park

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Endocrine disrupters refer to environmental or chemical compounds, which interfere with the endocrine system of organisms. In this study, our aim was to develop a screening method to detect xenoestrogen (an endocrine disrupter that is commonly encountered in our daily life) by using fission yeast Schizosaccharomyces pombe. Although the yeast (the simplest eukaryotic cell) has no endocrine system, estrogen receptors that are created to express in the yeast cell can be activated by estrogen in a similar manner to mammalian cells. First, in order to express the human estrogen receptor (hER) in the yeast cell, we constructed a yeast expression vector that contained hER (pREP42MHN-hER). In the yeast cells that are transformed with the pREP42MHN-hER vector, estrogen receptors could recognize xenoestrogen, which allowed the determination of the presence of xenoestrogen in any given sample. Furthermore, we constructed a yeast strain that contained an estrogen responsive element (ERE) that fused with the Escherichia coli LacZ gene (pERE-LacZ) as a reporter for binding of xenoestrogen with the estrogen receptor. Since this vector system allows determination of the presence and level of xenoestrogen with simple procedures, it is expected that they can serve as an efficient assay system to detect xenoestrogen.

Original languageEnglish
Pages (from-to)148-153
Number of pages6
JournalMolecules and Cells
Volume13
Issue number1
Publication statusPublished - 2002 Feb 1

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Schizosaccharomyces
Estrogen Receptors
Yeasts
Endocrine System
Estrogens
Lac Operon
Eukaryotic Cells
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Yoo, Eung Jae ; Jang, Yeun Kyu ; Kimm, Hyun Soo ; Choi, Eun Sik ; Park, Sang Dai. / Development of a new xenoestrogen screening system using fission yeast Schizosaccharomyces pombe. In: Molecules and Cells. 2002 ; Vol. 13, No. 1. pp. 148-153.
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Development of a new xenoestrogen screening system using fission yeast Schizosaccharomyces pombe. / Yoo, Eung Jae; Jang, Yeun Kyu; Kimm, Hyun Soo; Choi, Eun Sik; Park, Sang Dai.

In: Molecules and Cells, Vol. 13, No. 1, 01.02.2002, p. 148-153.

Research output: Contribution to journalArticle

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