Development of a novel two-dimensional directed differentiation system for generation of cardiomyocytes from human pluripotent stem cells

Sung Hwan Moon, Kiwon Ban, Changhoon Kim, Sang Sung Kim, Jaemin Byun, Ming Ke Song, In Hyun Park, Shan Ping Yu, Young Sup Yoon

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Human pluripotent stem cells (hPSCs) hold great promise for treating ischemic heart disease. However, current protocols for differentiating hPSCs either result in low yields or require expensive cytokines. Methods: Here we developed a novel two dimensional (2D) stepwise differentiation system that generates a high yield of cardiomyocytes (CMs) from hPSCs without using special cytokines. Initially, undifferentiated hPSCs were transferred onto Matrigel-coated plates without forming embryoid bodies (EBs) for a few days and were cultured in bFGF-depleted human embryonic stem cells (hESCs) medium. When linear cell aggregation appeared in the margins of the hPSC colonies, the medium was changed to DMEM supplemented with 10% fetal bovine serum (FBS). Thereafter when cell clusters became visible, the medium was changed to DMEM with 20% FBS. Results and conclusions: At about two weeks of culture, contracting clusters began to appear and the number of contracting clusters continuously increased, reaching approximately 70% of all clusters. These clusters were dissociated by two-step enzyme treatment to monolayered CMs, of which ~ 90% showed CM phenotypes confirmed by an α-myosin heavy chain reporter system. Electrophysiologic studies demonstrated that the hPSC-derived CMs showed three major CM action potential types with 61 to 78% having a ventricular-CM phenotype. This differentiation system showed a clear spatiotemporal role of the surrounding endodermal cells for differentiation of mesodermal cell clusters into CMs. In conclusion, this system provides a novel platform to generate CMs from hPSCs at high yield without using cytokines and to study the development of hPSCs into CMs.

Original languageEnglish
Pages (from-to)41-52
Number of pages12
JournalInternational Journal of Cardiology
Volume168
Issue number1
DOIs
Publication statusPublished - 2013 Sep 20

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

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