Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture

Sung Jae Shin, Yung Fu Chang, Cathy Huang, Jiaqian Zhu, Lester Huang, Han Sang Yoo, Kwang Soon Shin, Susan Stehman, Sang J. Shin, Alfonso Torres

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A polymerase chain reaction (PCR) assay for confirmation of Mycobacterium avium subsp. paratuberculosis was developed using the primer set derived from ISMav2. The PCR product was 494 base pairs (bp) and could be digested with ClaI, which produced 311- and 183-bp fragments. No amplification of 494-bp DNA fragment was detected from DNA of other Mycobacterium spp., including Mycobacterium avium complex, other bacteria, including Escherichia coli, Pseudomonas aeruginosa, Actinobacillus pleuropneumoniae, Leptospira interrogans serovar pomona, Corynebacterium pseudotuberculosis, Salmonella typhimurium, Borrelia burgdorferi, and Staphylococcus aureus, and the Scedosporium sp. This PCR assay could detect 5-8 genome equivalents.

Original languageEnglish
Pages (from-to)116-120
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume16
Issue number2
Publication statusPublished - 2004 Mar 1

Fingerprint

Paratuberculosis
Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis
Base Pairing
polymerase chain reaction
Polymerase Chain Reaction
Leptospira interrogans serovar pomona
Corynebacterium pseudotuberculosis
Scedosporium
Actinobacillus pleuropneumoniae
Mycobacterium avium complex
Mycobacterium avium Complex
Leptospira interrogans
Borrelia burgdorferi
DNA
testing
Mycobacterium
assays
Salmonella typhimurium
Pseudomonas aeruginosa

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Shin, Sung Jae ; Chang, Yung Fu ; Huang, Cathy ; Zhu, Jiaqian ; Huang, Lester ; Yoo, Han Sang ; Shin, Kwang Soon ; Stehman, Susan ; Shin, Sang J. ; Torres, Alfonso. / Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture. In: Journal of Veterinary Diagnostic Investigation. 2004 ; Vol. 16, No. 2. pp. 116-120.
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Shin, SJ, Chang, YF, Huang, C, Zhu, J, Huang, L, Yoo, HS, Shin, KS, Stehman, S, Shin, SJ & Torres, A 2004, 'Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture', Journal of Veterinary Diagnostic Investigation, vol. 16, no. 2, pp. 116-120.

Development of a polymerase chain reaction test to confirm Mycobacterium avium subsp. paratuberculosis in culture. / Shin, Sung Jae; Chang, Yung Fu; Huang, Cathy; Zhu, Jiaqian; Huang, Lester; Yoo, Han Sang; Shin, Kwang Soon; Stehman, Susan; Shin, Sang J.; Torres, Alfonso.

In: Journal of Veterinary Diagnostic Investigation, Vol. 16, No. 2, 01.03.2004, p. 116-120.

Research output: Contribution to journalArticle

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AU - Chang, Yung Fu

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AU - Huang, Lester

AU - Yoo, Han Sang

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AU - Stehman, Susan

AU - Shin, Sang J.

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