Gut microbiota dysbiosis and intestinal epithelial cell barrier dysfunction are associated with diseases such as inflammatory bowel disease and type 2 diabetes. To study the interaction of gut microbes and intestinal epithelial cells, in vitro model systems for co-culturing intestinal cells and gut microbes are essential. Since most gut microorganisms are anaerobic bacteria, it is difficult to co-culture microbes with intestinal epithelial cells that require oxygen during culture. We have developed a novel gut microbialepithelial cell (GMEC) co-culture system, a microfluidic chip in an anaerobic chamber with oxygen gradient within the chip. Appropriate co-culture flow rates were determined based on the experimental observation as well as fluid dynamics and molecular transport simulations. Using GMEC system, Escherichia coli and Lactobacillus plantarum were co-cultured with gut epithelial cell line, Caco-2. We expect that our GMEC co-culture system can potentially be a useful platform for studying the interaction between intestinal epithelial cells and gut microbes.
|Number of pages||13|
|Journal||Biotechnology and Bioprocess Engineering|
|Publication status||Published - 2022 Apr|
Bibliographical noteFunding Information:
This work was supported by National Research Foundation of Korea, under Grant (Basic Research Lab, 2019R1A4A 1025958 and 2022R1A4A2000748), and by the Ministry of Trade, Industry & Energy (MOTIE), Republic of Korea, under the Technology Innovation Program, Grant 20008413 and 20008414, Hongik University Research Fund, and 2021 Hongik University Innovation Support Program Fund.
© 2022, The Korean Society for Biotechnology and Bioengineering and Springer.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology
- Biomedical Engineering